Immunohistochemistry And tumor Markers Flashcards
It is the identification of specific or selective tissue or cellular antigens found on tissues
Immunohistochemistry
Controls in immunohistochemistry
Positive control
Negatuve control
Internal tissue control
Also known as built in control
Internal tissue control
A control that is a section of proven and known to have the antigen in question
Positive control
A control that omits the primary antibody from the staining schedule
Negative control
Tyoe of label that uses substrates with chromogen such as Diaminobenzidine(dark brown)
Enzyme labels
A label using fluorescein isothiocyanate
Fluorochrome labels
EMA stands for
Epithelial membrane antigen
A tumor marker for epithelial cells
Keratin
Tumor marker that distinguishes lung adenocarcinoma from mesothelioma
TTF-1 or Thyroid Transcription Factor - 1
Most specific tumor marker only found in the prostate
PSA or Prostate Specific Antigen
Tumor marker for determining the site of tumor
EMA
Marker for colon cancer
CEA or Carcinoemrbyonic Antigen
Breast cancer marker
ER/PR
Tumor marker highly specific for myogenic tumors
Desmin
Tumor marker that is normally expressed by CNS glial cells especially astrocytes
GFAP (Glial Fibrillary Acidic Protein)
A germ cell tumor marker that detects early pregnancy
HCG (Human Chorionic Gonadotropin)
Tumor marker synthesized by liver
AFP (Alpha-Fetoprotein)
Tumir marker detected in late pregnancy
Placental-like alkaline antigen (PLAP)
Described as tumors if skeletal muscle origin
Myogenic tumors
LCA (Leukocyte Common Antigen) is also known as
CD45
