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Histopathology > Fishing out > Flashcards

Fishing out Flashcards

1
Q

How is fishing out done?

A

Placing the ribbon of sections into the water bath for it to flatten

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2
Q

How long does it take for a tissue to flatten

A

30 seconds

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3
Q

At what angle is the slide stood to drain?

A

60-85 degrees

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4
Q

How long is the slide stood and drained

A

2-5 minutes

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5
Q

Methods to dry the section:

A
  1. Hot plate
  2. Leaving in incubator
  3. Wax oven
  4. Blower type electric slide dryer
  5. Above a bunsen burner
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6
Q

Urgent method of drying a section

A

Above a bunsen burner

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7
Q

How long can you dry a section in using Blower tyoe electric slide dryer?

A

20-30 mins

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8
Q

How long can you dry a section in using wax oven?

A

2 hours

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9
Q

How long can you dry a section in using incubator?

A

Overnight

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10
Q

How long can you dry a section in using hot pkate?

A

30-45 minutes

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11
Q

Temperature for drying a section in using hot plate

A

45-55 C

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12
Q

Temperature for drying a section in using wax oven

A

56-60 C

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13
Q

Temperature for drying a section in using blower type electric dryer

A

50-55 C

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14
Q

A drying method BEST for Nervous Tissue

A

Leaving in incubator overnight

Incubator

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15
Q

Difficulties during sectioning

A
  1. Mushy tissue block
  2. Tearing of tissue from block
  3. Sections fail to form ribbon
  4. Sections roll up on cutting, wrinkle or jammed
  5. Sections adhere to knife or other parts of microtome
  6. Chatters
  7. Floaters and contaminants
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16
Q

Tissue adhesives

A
  1. Albumin (mayer’s egg albumin)
  2. Gelatin
  3. Cellulose
  4. Poky-L-lysine
  5. Sodium silicate
  6. Resins
17
Q

Most commonly used tissue adhesive

A

Mayer’s egg albumin/albumin

18
Q

Added to Albumin to prevent molds

A

Thymol crystals

19
Q

A tissue adhesive that provides firmer attachment

A

Gelatin

20
Q

A tissue adhesive that us form of 1% Methyl cellulose

A

Cellulose

21
Q

A general purpose tissue adhesive

A

Poly-L-lysine

22
Q

Tissue adhesive for immunohistochemistry

A

Poly-L-lysine

23
Q

A tissue adhesive that is a commercial syrup

A

Sodium silicate

24
Q

Tissue adhesive with greatest adhesion

A

Resins

25
Q

Made of epoxy resins

A

Araldite

Histopathology (9 decks)

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