Fishing out Flashcards
How is fishing out done?
Placing the ribbon of sections into the water bath for it to flatten
How long does it take for a tissue to flatten
30 seconds
At what angle is the slide stood to drain?
60-85 degrees
How long is the slide stood and drained
2-5 minutes
Methods to dry the section:
- Hot plate
- Leaving in incubator
- Wax oven
- Blower type electric slide dryer
- Above a bunsen burner
Urgent method of drying a section
Above a bunsen burner
How long can you dry a section in using Blower tyoe electric slide dryer?
20-30 mins
How long can you dry a section in using wax oven?
2 hours
How long can you dry a section in using incubator?
Overnight
How long can you dry a section in using hot pkate?
30-45 minutes
Temperature for drying a section in using hot plate
45-55 C
Temperature for drying a section in using wax oven
56-60 C
Temperature for drying a section in using blower type electric dryer
50-55 C
A drying method BEST for Nervous Tissue
Leaving in incubator overnight
Incubator
Difficulties during sectioning
- Mushy tissue block
- Tearing of tissue from block
- Sections fail to form ribbon
- Sections roll up on cutting, wrinkle or jammed
- Sections adhere to knife or other parts of microtome
- Chatters
- Floaters and contaminants
Tissue adhesives
- Albumin (mayer’s egg albumin)
- Gelatin
- Cellulose
- Poky-L-lysine
- Sodium silicate
- Resins
Most commonly used tissue adhesive
Mayer’s egg albumin/albumin
Added to Albumin to prevent molds
Thymol crystals
A tissue adhesive that provides firmer attachment
Gelatin
A tissue adhesive that us form of 1% Methyl cellulose
Cellulose
A general purpose tissue adhesive
Poly-L-lysine
Tissue adhesive for immunohistochemistry
Poly-L-lysine
A tissue adhesive that is a commercial syrup
Sodium silicate
Tissue adhesive with greatest adhesion
Resins
Made of epoxy resins
Araldite
