Frozen sections Flashcards
Learn about methods and principle of Frozen sections
Frozen Section is used for:
- Rapid Diagnosis
- For enzyme Histochemistry
- Demonstration of soluble substances
- Immunofluorescent and Immunocytochemical staining
- Specialized silver stains
2 methods of frozen section
Cold Knife & Cryostat
A method that uses Conventional Freezing Microtome (CO2)
Cold Knife
It is the term used to describe the point in which sections may be vut at 10 um
Dew Line
A cold knife can produce a ____ thick tissue block
3-5 mm
A method that uses Cryostat
Cryostat
Optimum temperature for Cryostat
-18 to -20 C
Average: -20C
Methods of Freezing (5)
Liquid Nitrogen Isopentane Aerosol sprays Carbon dioxide Freon 2.2
Most rapid freezing agent
Liquid Nitrogen
At what temp is Isopentane liquid?
Room temp (22-25C)
Temperature in Carbon dioxide freezing agent
-50 C
Temperature in Liquid Nitrogen freezing agent
-190C
Clearing agents used in frozen sections
Glycerin Gum syrup (water based)
What process is not necessary in Frozen sections
Dealcoholization
Function of clearing agent
Increase the refractive index
What is used in mounting frozen sections
Synthethic water soluble glycols and resins
Tissue section produce in frozen sections
5-10 um
Size of tissue block in F.S.
2-4 mm
