Frozen sections Flashcards
Learn about methods and principle of Frozen sections
1
Q
Frozen Section is used for:
A
- Rapid Diagnosis
- For enzyme Histochemistry
- Demonstration of soluble substances
- Immunofluorescent and Immunocytochemical staining
- Specialized silver stains
2
Q
2 methods of frozen section
A
Cold Knife & Cryostat
3
Q
A method that uses Conventional Freezing Microtome (CO2)
A
Cold Knife
4
Q
It is the term used to describe the point in which sections may be vut at 10 um
A
Dew Line
5
Q
A cold knife can produce a ____ thick tissue block
A
3-5 mm
6
Q
A method that uses Cryostat
A
Cryostat
7
Q
Optimum temperature for Cryostat
A
-18 to -20 C
Average: -20C
8
Q
Methods of Freezing (5)
A
Liquid Nitrogen Isopentane Aerosol sprays Carbon dioxide Freon 2.2
9
Q
Most rapid freezing agent
A
Liquid Nitrogen
10
Q
At what temp is Isopentane liquid?
A
Room temp (22-25C)
11
Q
Temperature in Carbon dioxide freezing agent
A
-50 C
12
Q
Temperature in Liquid Nitrogen freezing agent
A
-190C
13
Q
Clearing agents used in frozen sections
A
Glycerin Gum syrup (water based)
14
Q
What process is not necessary in Frozen sections
A
Dealcoholization
15
Q
Function of clearing agent
A
Increase the refractive index
