Fixation Flashcards
1
Q
The most crucial step
A
Fixation
2
Q
A process of preserving cells and tissue
A
Fixation
3
Q
2 important goals of fixation
A
- Preserve the morphological and chemical integrity of the cell.
- Harden the tissue for easy handling
4
Q
Two mechanisms of action in fixation
A
- Additive fixation
2. Non-additive fixation
5
Q
A mechanism of action that does not incorporate the fixative to the tissue
A
Non-additive fixation
6
Q
A mechanism of action that make the fixative a part of the tissue
A
Additive fixation
7
Q
Factors involved in fixation
A
- pH
- Temperature
- Thickness
- Osmolality
- Concentration
- Time duration
8
Q
pH in fixation must be
A
6-8
9
Q
Traditional tempersture in fixation
A
Room temp or 22-25C
10
Q
Time duration in fixation
A
2-6 hrs
11
Q
Types of fixative
A
- Simpke fixatives
2. Compount fixative
12
Q
A type of fixative that has 2 or more fixative
A
Compount fixative
13
Q
Type of fixative with 1 component
A
Simpke fixative
14
Q
Fixative classifications according to action
A
- Microanatomical
2. Cytological
15
Q
Aldehyde fixatives
A
- Formaline (formaldehyde)
- Formol Corrosive (formol sublimate)
- Glutaraldehyde
- 10% Formol saline
- 10% Neutral Buffered Formalin/ PO4 Buffered formalin
- Gendre’s/ alcoholic formalin
- Paraformaldehyde
