Mounting

Question 1

mounting is a ____ applied between the section and the coverslip after staining setting the section firmly, preventing the movement of the coverslip

Answer 1

syrupy fluid

Question 2

why mounting is ncecessary?

Answer 2

to protect stained section from physical injury and from bleaching or deterioration of the stain as result of oxidation

Question 3

for best results with stained tissue sections, they must be impregnated by a ___ medium having a ___ close to that of glass (___).

Answer 3

impregnated by a transparent medium having a refractive index close to that of glass (1.518).

Question 4

most commonly used mounting media

Answer 4

Eukitt & Entellan

Question 5

designed to mount ___ preparations directly from water in cases where the stain is removed or decolorized with alcohol or xylene

Answer 5

water-miscible preparations

AQUEOUS/WATER SOLUBLE MOUNTANTS

Question 6

enumerate the aqueous/water soluble mountants

Answer 6

• water
• glycerin (RI:147)
• farrant’s medium (gum syrup) (RI:1.43)
• apathy’s medium
• brun’s fluid

Question 7

this aqueous/water soluble mountants has low refractive index good only for temporary mounting

Answer 7

water

Question 8

this aqueous/water soluble mountants has high refractive index which is also used as a preservative

Answer 8

glycerin

Question 9

refractive index of glycerin

Answer 9

1.47

Question 10

a gum syrup that does not solidify upon storage

Answer 10

farrant’s medium

Question 11

refractive index of farrant’s medium

Answer 11

1.43

Question 12

used for methylene-blue stained nerve preparations and as general purpose aqueous mountant

Answer 12

apathy’s medium

Question 13

this aqueous/water soluble mountants is recommended for mounting frozen sections from water or paraffin sections which require dehydration and clearing

Answer 13

brun’s fluid

Question 14

this is used for preparations that have been dehydrated and cleared in xylene or toluene

Answer 14

resinous mountants

Question 15

recommended for majority of staining methods

Answer 15

• Canada Balsam
• DPX
• XAM
• Clarite

Question 16

Canada Balsam is a natural resin extracted from the ____

Answer 16

Canadian tree (Abus balsamea)

Question 17

refractive index of Canada Balsam

Answer 17

1.524

Question 18

recommended for whole mounts and for thick sections because it does not shrink much

Answer 18

Canada Balsam

Question 19

it is miscible with xylene and is quite expensive

Answer 19

Canada balsam

Question 20

this is recommended for small tissue sections

Answer 20

DPX

Question 21

refractive index of PDX

Answer 21

1.532

Question 22

a synthetic resin mixture in xylene in pale yellow or colorless solution

Answer 22

XAM

Question 23

refractive index of XAM

Answer 23

1.52

Question 24

a synthetic resin which is soluble in xylene and is usually diluted to 60% with xylene

Answer 24

clarite

Question 25

refractive index of clarite

Answer 25

1.544

Question 26

process of sealing the margins of the coverslip to prevent escape of fluid or semi-fluid mounts and evaporation of mountant, to immobilize the coverslip and to prevent sticking of slides upon storage

Answer 26

ringing

Question 27

made up of 2 parts paraffin wax mixed with 4-9 parts powdered colophonium resin, heated and filtered

Answer 27

kronig cement