Mounting Flashcards
mounting is a ____ applied between the section and the coverslip after staining setting the section firmly, preventing the movement of the coverslip
syrupy fluid
why mounting is ncecessary?
to protect stained section from physical injury and from bleaching or deterioration of the stain as result of oxidation
for best results with stained tissue sections, they must be impregnated by a ___ medium having a ___ close to that of glass (___).
impregnated by a transparent medium having a refractive index close to that of glass (1.518).
most commonly used mounting media
Eukitt & Entellan
designed to mount ___ preparations directly from water in cases where the stain is removed or decolorized with alcohol or xylene
water-miscible preparations
AQUEOUS/WATER SOLUBLE MOUNTANTS
enumerate the aqueous/water soluble mountants
- water
- glycerin (RI:147)
- farrant’s medium (gum syrup) (RI:1.43)
- apathy’s medium
- brun’s fluid
this aqueous/water soluble mountants has low refractive index good only for temporary mounting
water
this aqueous/water soluble mountants has high refractive index which is also used as a preservative
glycerin
refractive index of glycerin
1.47
a gum syrup that does not solidify upon storage
farrant’s medium
refractive index of farrant’s medium
1.43
used for methylene-blue stained nerve preparations and as general purpose aqueous mountant
apathy’s medium
this aqueous/water soluble mountants is recommended for mounting frozen sections from water or paraffin sections which require dehydration and clearing
brun’s fluid
this is used for preparations that have been dehydrated and cleared in xylene or toluene
resinous mountants
recommended for majority of staining methods
- Canada Balsam
- DPX
- XAM
- Clarite
Canada Balsam is a natural resin extracted from the ____
Canadian tree (Abus balsamea)
refractive index of Canada Balsam
1.524
recommended for whole mounts and for thick sections because it does not shrink much
Canada Balsam
it is miscible with xylene and is quite expensive
Canada balsam
this is recommended for small tissue sections
DPX
refractive index of PDX
1.532
a synthetic resin mixture in xylene in pale yellow or colorless solution
XAM
refractive index of XAM
1.52
a synthetic resin which is soluble in xylene and is usually diluted to 60% with xylene
clarite
refractive index of clarite
1.544
process of sealing the margins of the coverslip to prevent escape of fluid or semi-fluid mounts and evaporation of mountant, to immobilize the coverslip and to prevent sticking of slides upon storage
ringing
made up of 2 parts paraffin wax mixed with 4-9 parts powdered colophonium resin, heated and filtered
kronig cement
