fixatives uses

Question 1

satisfactory for routine paraffin sections

Answer 1

aldehyde fixatives

Question 2

for central nervous tissue and postmortem tissues

Answer 2

10% formol saline

Question 3

recommended for preservation and storage of surgical, post-mortem and research specimens

Answer 3

10% neutral buffered formalin or phosphate buffered formalin

Question 4

recommended for routine post-mortem tissues

Answer 4

formal-corrosive

Question 5

good for preservation of glycogen and micro-incineration technique

used to fix sputum since it coagulates mucus

Answer 5

alcoholic formalin (genre’s fixative)

Question 6

glutaraldehyde solution used for small fragments and needle biopsies fixed in 2-4 hrs at room temp

Answer 6

2.5% solution

Question 7

glutaraldehyde solution used for larger tissues fixed in 6-8 hrs up to 24 hrs

Answer 7

4% solution

Question 8

routine fixative of choice for preservation of cell detail in tissue photography

Answer 8

mercuric chloride

most common metalic fixative

Question 9

recommended for fixing small pieces of liver, spleen, connective tissue fibers and nuclei

Answer 9

zenker’s fluid

Question 10

excellent microanatomic fixative for pituitary gland, bone marrow, and blood containing organs such as spleen and liver

Answer 10

zenker-formol (helly’s solution)

Question 11

main fixative for tumor biopsies especially the skin. excellent cytologic fixative

Answer 11

heidenhain’s susa

Question 12

used for bone marrow biopsies

Answer 12

Lillie’s b5 fixative

Question 13

precipitates all proteins and adequately preserves carbohydrates

Answer 13

chromic acid

Question 14

preserves lipids and preserves mitochondria

Answer 14

potassium dichromate

Question 15

recommended for demonstration of chromatin, golgi bodies, BC, and colloid containing tissues

Answer 15

regaud’s (muller) fluid

Question 16

recommended for early degenerative process and tissue necrosis

Answer 16

orth’s fluid

Question 17

demonstrates rickketsia and other bacteria

Answer 17

orth’s fluid

Question 18

recommended for fixation of embryos and pituitary biopsies

Answer 18

bouin’s solution

Question 19

normally used in conjunction with other fixatives to form a compound solution

Answer 19

glacial acetic acid

Question 20

denatures and precipitates proteins by destroying hydrogen and other bonds. may be used both as fixative and dehydrating agent

Answer 20

alcohol fixatives

Question 21

excellent for fixing dry and wet smears, blood smears and bone marrow biopsies

Answer 21

methyl alcohol 100%

Question 22

used for fixing touch preparations

Answer 22

isopropyl alcohol 95%

Question 23

recommended for fixing chromosomes, lymph glands, and urgent biopsies

Answer 23

carnoy’s fluid

Question 24

recommended for fixing mucopolysaccharides and nuclear proteins

Answer 24

newcomer’s fluid

Question 25

fixation with this solution causes complete denaturation of protein

Answer 25

osmium tetroxide

Question 26

most common chrome-osmium acetic acid fixative uses

Answer 26

flemming’s solution

Question 27

excellent fixative for nuclear structures and permanently fixes fat

Answer 27

flemming’s solution

Question 28

recommended for cytoplasmic strucutres

Answer 28

flemming’s solution without acetic acid

Question 29

recommended for study of water diffusible enzymes especially phosphatases and lipases

used in fixing brain tissues for diagnosis of rabies

Answer 29

acetone

Question 30

usually for frozen tissue sections and preparation of bacteriologic smears

Answer 30

heat fixation