Monoclonal Antibodies

Question 1

Describe how monoclonal antibodies are produced

Answer 1

1. A mouse is injected with an antigen🐁
2. This is detected by the immune system and stimulate B lymphoctes to prouce the specific antibodies
3. The lymphocyte are taken out of the mouse and fused with tumour cells to prodcue hybridoma cells- the hybridoma cells can produce the specific antibodies and divide my mitosis
4. Then a single hybridoma is selected and allowed to divide by mitosis to produce clones of identical hybridoma cells
5. The hybridoma all produce identical and specific antibodies called monoclonal antibodies
6. The monoclonal antibodies are then collected and purified

Question 2

What is a monoclonal antibody?

Answer 2

Antibodies produced from a single clone of identical hybridoma cells

Question 3

Monoclonal antibodies are [blank] to one [blank] on one [blank]

Answer 3

Monocloanl antibodies and specific to one binding site on one protein antigen

to one…on one