Molecular Diagnostics

Question 1

How many restriction sites are there in normal Beta-globulin? How many are in Sickle Cell Anemia?
What testing method is being used?

Answer 1

normal Beta-Globulin uses 3 Ddel restriction sites.
Sickle cell anemia uses 2 Ddel restriction sites.
The method is called RFLP(Restriction Fragment Length Polymorphism).

Question 2

For what purpose is VNTR method used?

Answer 2

Used in identification and severity of inherited diseases such as Huntington disease, Fragile X Syndrome

Question 3

What is being inserted into expression vectors during production of recombinant proteins?

Answer 3

cDNA

Question 4

What does indirect Elisa measure? Give one specific example of its use

Answer 4

Measures amount of antibody. It is used in diagnosing HIV.

Question 5

What does Sandwich Elisa measure? Give specific example

Answer 5

Measures amount of antigen in a sample: 2 antibodies sandwich an antigen. It is used in detecting Troponin for MI and pregnancy test (HcG).

Question 6

What is SDS-Page used for?

Answer 6

Separate protein on a gel by applying electrical field. Small proteins move faster.