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HEMA BLOOD CELL ANOMALIES > MOLBIO > Flashcards

MOLBIO Flashcards

1
Q

The branch of biology that studies the structure, composition, and interactions of cellular biomolecules

A

Molecular biology

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2
Q

Deals with molecular techniques that are designed for handling and analysis of nucleic acids
- it is rooted in the understanding of genome structure and cellular activities at the molecular level

A

Diagnostic Molecular Biology

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3
Q
  • accurate and error free
  • total process guaranteeing accuracy of final results reported
  • systemic measurement , comparison with a standard
A

QUALITY ASSURANCE

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4
Q

State the 3 Importance of quality assurance

A
  1. Results impact management
  2. Laboratory faces issues and challenges in daily operations
  3. Most critical quality issue in the laboratory is contamination
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5
Q

Contamination sources:

A
  1. Environment
  2. Other specimen and amplicans
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6
Q

These are products of amplification

A

Amplicans

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7
Q

What are the contamination effects?

A

False negative results
False positive results

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8
Q

What are the five components considered in MDBX

A
  1. Personnel requirements
  2. Facilities and workflow
  3. Assay requirements
  4. External quality assurance program
  5. Quality assurance
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9
Q

True or false:
staffs must undergo general orientations and trainings

A

FALSE: New hires must undergo general orientations and trainings

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10
Q

PERSONNEL REQUIREMENTS:
Staff must display____ and display evidence of ____

A

Proficiency testing and proficiency

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11
Q

TRUE OR FALSE: Personnel requirements must satisfy quantity requirements

A

Quality requirements

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12
Q

Procedure performed in different rooms
- separation of work areas/unidirectional workflow

A

SPACE AND TIME PREPARATION

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13
Q

UNIDIRECTIONAL WORKFLOW SEQUENCE

A

Reagent > sample> amplification > post amplification

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14
Q

Clean rooms have? And dirty rooms have?

A

Clean rooms: positive air pressure
Dirty rooms: negative air pressure

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15
Q

What are the laboratory practices?

A
  • use of appropriate PPE in the performance of laboratory tasks
  • decontamination in procedures for staff, work areas, equipment supplies
  • employ techniques that eliminate contamination
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16
Q

Chemical control:
True of false:
Surface decontamination by 10% alcohol and 70% bleach solution

A

TRUE OR FALSE:
Surface decontamination by 70% alcohol and 10% bleach solution

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17
Q

Chemical control:

A
  • Surface decontamintaion by 70% alcohol and 10% bleach solution
  • usage of bleach-based decontaminating agents
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18
Q

ENVIRONMENTAL CONTROL:

A
  1. Airflow must be considered
  2. Avoid unnecessary movements/ activities by the staff
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19
Q

What are the 4 components under facilities and workflow?

A
  1. Space and time preparation
  2. Laboratory practices
  3. Chemical control
  4. Environmental control
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20
Q

What are the 3 assay requirements?

A
  1. Clinical specimen
  2. Reagents, control and standards
  3. Equipment maintenance
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21
Q

Specimen requirements (_____, _____, and ______) must be allowed

A

Type, volume and collection procedure

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22
Q

Proper transport and storage procedures

A

Clinical specimen

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23
Q

Acquire supplies from reliable sources

A

Reagents, control and standards

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24
Q

Relevant details of supplies should be noted and documented

A

Regents, control and standards

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25
Q

Performance of supplies should be monitored

A

Reagents, control, and standards

26
Q

Must be acquired from reliable sources:

A

Reagents, control, and standards
Equipment maintenance

27
Q

Usage should be logged

A

Equipment maintenance

28
Q

Onsite calibration/ validation and regular preventive maintain should be on schedule

A

Equipment maintenance

29
Q

Evaluation of laboratory’s performance on examination of samples of external origin for the purpose of examining the lab’s pre- exam examination and post examination

A

External quality assurance program

30
Q

What are the components under the external quality assurance program

A
  1. Proficiency testing
  2. Concordance testing
  3. Laboratory assessment
  4. Laboratory staff competency assessment
  5. Simple referral and turn around time
31
Q

Set of procedures undertaken by the staff for continuously assessing laboratory work and the emergent results in order to decide whether results are reliable enough to be continued

A

Quality assurance

32
Q

Measures that must be included during each assay run to verify that the test is working properly

A

Quality assurance

33
Q

2 use of assay controls

A
  1. Extraction control
  2. PCR control
34
Q

Name the 4 controls under the PCR control

A

Internal control
Positive control
Negative control
No template control

35
Q

Containment principles, technologies, and principles implemented to prevent unintentional exposure to pathogens and toxins and their unintentional release

A

Laboratory biosafety

36
Q

Protection, control and accountability for valuable biological materials within laboratories in order to prevent their:
Unauthorized access
Loss
Theft
Misuse diversion
Unintentional release

A

Laboratory biosecurity

37
Q

System or process to control safety and security risks associated with the handling or storage and disposal of biological agents and toxins in laboratories and facilities

A

Laboratory biorisk management

38
Q

What are the major steps in laboratory risk management

A

Assessment
Mitigation
Performance

39
Q

What are the detailed steps jn the assessment of laboratory risk management

A

Risk identification
Hazard/ threat identification
Likelihood evaluation
Consequences evaluation

40
Q

Mitigation detailed steps

A

Biorisk control measures
Risk management

41
Q

Perfromance detailed steps

A

Control
Assurance
Improvement

42
Q

Give the Three letter symbol and one letter symbol

Alanine

A

Three letter symbol: Ala
One letter symbol: A

43
Q

Arginine

A

Arg
R

44
Q

Asparagine

A

Asn
N

45
Q

Aspartic acid

A

Asp
D

46
Q

Cysteine

A

Cys
C

47
Q

Glutamic acid

A

Glu
E

48
Q

Glutamine

A

Gln
Q

49
Q

Histidine

A

His
H

50
Q

Isoleucine

A

Ile
I

51
Q

Leucine

A

Leu
L

52
Q

Lysine

A

Lys
K

53
Q

Methionine

A

Met
M

54
Q

Phenylalanine

A

Phef

55
Q

Proline

A

Pro
P

56
Q

Serine

A

Ser
S

57
Q

Threonine

A

Thr
T

58
Q

Tryptophan

A

Trp
W

59
Q

Tyrosine

A

Tyr
Y

60
Q

Valine

A

Val
V

61
Q

Start codon

A

AUG

62
Q

Stop codon

A

AUU
UAG
UGA

HEMA BLOOD CELL ANOMALIES (8 decks)

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