MOLBIO Flashcards
The branch of biology that studies the structure, composition, and interactions of cellular biomolecules
Molecular biology
Deals with molecular techniques that are designed for handling and analysis of nucleic acids
- it is rooted in the understanding of genome structure and cellular activities at the molecular level
Diagnostic Molecular Biology
- accurate and error free
- total process guaranteeing accuracy of final results reported
- systemic measurement , comparison with a standard
QUALITY ASSURANCE
State the 3 Importance of quality assurance
- Results impact management
- Laboratory faces issues and challenges in daily operations
- Most critical quality issue in the laboratory is contamination
Contamination sources:
- Environment
- Other specimen and amplicans
These are products of amplification
Amplicans
What are the contamination effects?
False negative results
False positive results
What are the five components considered in MDBX
- Personnel requirements
- Facilities and workflow
- Assay requirements
- External quality assurance program
- Quality assurance
True or false:
staffs must undergo general orientations and trainings
FALSE: New hires must undergo general orientations and trainings
PERSONNEL REQUIREMENTS:
Staff must display____ and display evidence of ____
Proficiency testing and proficiency
TRUE OR FALSE: Personnel requirements must satisfy quantity requirements
Quality requirements
Procedure performed in different rooms
- separation of work areas/unidirectional workflow
SPACE AND TIME PREPARATION
UNIDIRECTIONAL WORKFLOW SEQUENCE
Reagent > sample> amplification > post amplification
Clean rooms have? And dirty rooms have?
Clean rooms: positive air pressure
Dirty rooms: negative air pressure
What are the laboratory practices?
- use of appropriate PPE in the performance of laboratory tasks
- decontamination in procedures for staff, work areas, equipment supplies
- employ techniques that eliminate contamination
Chemical control:
True of false:
Surface decontamination by 10% alcohol and 70% bleach solution
TRUE OR FALSE:
Surface decontamination by 70% alcohol and 10% bleach solution
Chemical control:
- Surface decontamintaion by 70% alcohol and 10% bleach solution
- usage of bleach-based decontaminating agents
ENVIRONMENTAL CONTROL:
- Airflow must be considered
- Avoid unnecessary movements/ activities by the staff
What are the 4 components under facilities and workflow?
- Space and time preparation
- Laboratory practices
- Chemical control
- Environmental control
What are the 3 assay requirements?
- Clinical specimen
- Reagents, control and standards
- Equipment maintenance
Specimen requirements (_____, _____, and ______) must be allowed
Type, volume and collection procedure
Proper transport and storage procedures
Clinical specimen
Acquire supplies from reliable sources
Reagents, control and standards
Relevant details of supplies should be noted and documented
Regents, control and standards
Performance of supplies should be monitored
Reagents, control, and standards
Must be acquired from reliable sources:
Reagents, control, and standards
Equipment maintenance
Usage should be logged
Equipment maintenance
Onsite calibration/ validation and regular preventive maintain should be on schedule
Equipment maintenance
Evaluation of laboratory’s performance on examination of samples of external origin for the purpose of examining the lab’s pre- exam examination and post examination
External quality assurance program
What are the components under the external quality assurance program
- Proficiency testing
- Concordance testing
- Laboratory assessment
- Laboratory staff competency assessment
- Simple referral and turn around time
Set of procedures undertaken by the staff for continuously assessing laboratory work and the emergent results in order to decide whether results are reliable enough to be continued
Quality assurance
Measures that must be included during each assay run to verify that the test is working properly
Quality assurance
2 use of assay controls
- Extraction control
- PCR control
Name the 4 controls under the PCR control
Internal control
Positive control
Negative control
No template control
Containment principles, technologies, and principles implemented to prevent unintentional exposure to pathogens and toxins and their unintentional release
Laboratory biosafety
Protection, control and accountability for valuable biological materials within laboratories in order to prevent their:
Unauthorized access
Loss
Theft
Misuse diversion
Unintentional release
Laboratory biosecurity
System or process to control safety and security risks associated with the handling or storage and disposal of biological agents and toxins in laboratories and facilities
Laboratory biorisk management
What are the major steps in laboratory risk management
Assessment
Mitigation
Performance
What are the detailed steps jn the assessment of laboratory risk management
Risk identification
Hazard/ threat identification
Likelihood evaluation
Consequences evaluation
Mitigation detailed steps
Biorisk control measures
Risk management
Perfromance detailed steps
Control
Assurance
Improvement
Give the Three letter symbol and one letter symbol
Alanine
Three letter symbol: Ala
One letter symbol: A
Arginine
Arg
R
Asparagine
Asn
N
Aspartic acid
Asp
D
Cysteine
Cys
C
Glutamic acid
Glu
E
Glutamine
Gln
Q
Histidine
His
H
Isoleucine
Ile
I
Leucine
Leu
L
Lysine
Lys
K
Methionine
Met
M
Phenylalanine
Phef
Proline
Pro
P
Serine
Ser
S
Threonine
Thr
T
Tryptophan
Trp
W
Tyrosine
Tyr
Y
Valine
Val
V
Start codon
AUG
Stop codon
AUU
UAG
UGA
