Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

HEMA BLOOD CELL ANOMALIES > AUBF LAB UNIT 2 MOD 3 > Flashcards

AUBF LAB UNIT 2 MOD 3 Flashcards

1
Q

The glucose reagent strip uses a ____ and detects glucose only

A

Double sequential enzyme reaction (glucose oxidase and peroxidase)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What catalyzes the oxidation of glucose to form hydrogen peroxide and gluconic acid

A

Glucose oxidase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

The hydrogen peroxide formed oxidizes the _____ on the pad into ___ in the presence of peroxidase, causing color changes from _________

A

Chromogen (potassium iodide); iodide; blue to green to brown

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is the chemical equation used in reagent strip

A

Glucose + O2 —-(glucose oxidase)- -> Gluconic acid + hydrogen peroxide

Hydrogen peroxide + chromogen —— (peroxidase) > oxidized chromogen + water

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Chemstrip is sensitive to a concentration of ___

A

40 mg/dl

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Multistix is sensitive to a concentration of ________

A

75-125 mg/dl

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

VChem is sensitive to a concentration of____

A

45 mg/dl

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

The sensitivity of the reagent strip is affected by?

A

Urine temperature
Ph
Specific gravity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Increase specific gravity and decreased temperature ____ the sensitivity

A

Decreases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

False positive reactions occur in the presence of strong oxidizing agents such as:

A

Sodium hypochlorite and peroxides

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

False negative reactions occur in the presence of :

A

Ascorbic acid at a concentration if > 50 mg/dl

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Copper reductio test is also known as?

A

Benedicts test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Reducing susbtsances in the benedicts test:

A

Glucose
Galactose
Lactose
Maltose
Pentose
Arabinose
Ribose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

The benedicts test depends on the sbility of reducing substances to convert _____ to ______ in the presence of _______ and _____

A

Cupric sulfate to cuprous oxide; alkaline environment and heat

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

The benedicts reagent is a combination of?

A

Copper sulfate
Sodium carbonate
Sodium citrate buffer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

False positive in benedicts test may occur in the presence of:

A

Ascorbic acid
Tetracyclines
Salicylate
Penicillin
Cysteine
Homogentisic acid
Glucoronate

17
Q

False negative in the benedicts test may occur in the presence of:

A

Radiographic contrast media and sulfonamides

18
Q

A tablet version of the benedicts test is the _____

A

Clinitest reagent tablet

19
Q

It is a self heating method for the semiquantitative determination of reducing substances in the urine

A

CLINITEST

20
Q

The clinitest reagent tablet contains the following reagents:

A

Copper sulfate
Citric acid
Sodium hydroxide
Sodium carbonate

HEMA BLOOD CELL ANOMALIES (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions