MODULE 8

Question 1

The migration of charged solutes/particles in an
electrical field

Answer 1

ELECTROPHORESIS

Question 2

Separates proteins based on their electric charge
densities.

Answer 2

ELECTROPHORESIS

Question 3

Also called “driving force,” “power
supply,” “electrical power” and it is source of power.

Answer 3

POWER SUPPLY

Question 4

Solution that resists changes in pH when acid or alkali is added to it.

Answer 4

BUFFER

Question 5

positively or negatively charged molecules (e.g. proteins)

Answer 5

AMPHOLYTES

Question 6

the most widely used buffers are made of

Answer 6

MONOVALENT IONS

Question 7

separates by molecular size

Answer 7

CELLULOSE ACETATE

Question 8

separates by electrical charge

Answer 8

AGAROSE GEL

Question 9

separates based on the charge and molecular size;
used to study isoenzymes.

Answer 9

POLYACRYLAMIDE GEL

Question 10

separated proteins based
on surface charge and molecular size

Answer 10

STARCH GEL

Question 11

The procedure is not widely used
because of technical difficulty in
preparing the gel.

Answer 11

STARCH GEL

Question 12

is the most common and
reliable way for quantitation.

Answer 12

DENSITOMETRY

Question 13

The simplest way to accomplish detection is
visualization under

Answer 13

UV LIGHT

Question 14

It utilizes nanoliter quantities of specimens

Answer 14

CAPILLARY ELECTROPHORESIS