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ANACHEM > MODULE 7 > Flashcards

MODULE 7 Flashcards

1
Q

Separation of soluble components in a solution by specific differences in physical-chemical characteristics of the different constituents.

A

CHROMATOGRAPHY

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2
Q

carries the complex mixture

A

MOBILE PHASE

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3
Q

through which the mobile phase flows

A

STATIONARY PHASE

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4
Q

– holding the stationary phase

A

COLUMN

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5
Q

combination of mobile phase
and analytes

A

ELUATE

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6
Q

portion of mobile phase

A

ELUENT

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7
Q

MODES OF SEPARATION

A
  1. Adsorption Chromatography
  2. Partition Chromatography
  3. Ion Exchange Chromatography
  4. Steric Exclusion/Size Exclusion
  5. Affinity Chromatography
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8
Q

is the process whereby a solute is transferred from a mobile to a stationary phase

A

SORPTION

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9
Q

Also known as “liquid-solid chromatography”

A

ADSORPTION CHROMATOGRAPHY

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10
Q

can be acidic polar (silica
gel), basic polar (alumina), nonpolar (charcoal)

A

STATIONARY PHASE

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11
Q

can be a single solvent or a
mixture of two or more solvents, depending on the analytes to be desorbed.

A

MOBILE PHASE

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12
Q

Also known as “liquid-liquid chromatography”

A

PARTITION CHROMATOGRAPHY

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13
Q

Separation of compounds is based on their partition between a liquid mobile phase and a liquid stationary phase coated on a sloid support.

A

PARTITION CHROMATOGRAPHY

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14
Q

It is for separation of therapeutic drugs and their metabolites.

A

PARTITION CHROMATOGRAPHY

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15
Q

The mechanism in this type of chromatography is the exchange of sample ions and mobile phase ions with the charged group of the stationary phase.

A

ION EXCGANGE CHROMATOGRAPHY

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16
Q

It is for separation of amino acids, proteins, and nucleic acids.

A

ION EXCGANGE CHROMATOGRAPHY

17
Q

It separates molecules based on differences in their size and shape.

A

SIZE EXCLUSION

18
Q

In the early methods, they used hydrophilic beads (agarose, polyacrylamide), it forms a gel once soaked in water.

A

GEL FILTRATION

19
Q

using hydrophobic gel beads of polystyrene with non-aqueous mobile phase was chromatography

A

GEL PERMIATION

20
Q

Also called “lock-and-key binding”

A

AFFINITY CHROMATOGRAPHY

21
Q

Used to transmit signals in between cells.

A

LIGANDS

22
Q

It is for separation of lipoproteins,
carbohydrates, and glycated hemoglobin; antibodies

A

AFFINITY CHROMATOGRAPHY

23
Q

TYPES OF PLANAR

A

PAPER CHROMATOGRAPHY
THIN LAYER CHROMATOGRAPHY

24
Q

two forms of chromatography

A

PLANAR
COLUMN

25
Q

TYPES OF
COLUMN

A

GAS CHROMATOGRAPHY
LIQUID CHROMATOGRAPHY

26
Q

It is used for fractionation of sugar and
amino acids.

A

PAPER CHROMATOGRAPHY

27
Q

Semi quantitative drug screening test and urine drug screening test

A

THIN LAYER CHROMATOGRAPHY

28
Q

Separation of steroids, barbiturates, blood, alcohol, and lipids.

A

GAS CHROMATOGRAPHY

29
Q

Also called “downers” – to help sleep, relieve anxiety and muscle spasm, and
prevent seizures

A

BARBITURATES

30
Q

Separation occurs based on differences
in adsorption at the solid phase surfaces.

A

Gas-Solid Chromatography

31
Q

separation occurs by differences in solute partitioning between the gaseous
mobile phase and the liquid stationary
phase.

A

Gas-Liquid Chromatography

32
Q

Commonly used in Clinical Laboratory.

A

Gas-Liquid Chromatography

33
Q

Based on the distribution of solutes between a liquid mobile phase and a stationary phase.

A

LIQUID CHROMATOGRAPHY

34
Q

Most widely used liquid chromatography.

A

HIGH PERFORMANCE - LIQUID CHROMATOGRAPHY

35
Q

Is for detecting non-volatile substances I
body fluids.

A

Liquid Chromatography - Mass
Spectroscopy (LC-MS)

36
Q

It is utilized to conform positive results
from screening of elicited drugs.

A

Liquid Chromatography - Mass
Spectroscopy (LC-MS)

37
Q

Based on the fragmentation and
ionization of molecules using a suitable
source of energy.

A

MASS SPECTROSCOPY

38
Q

the gold standard for drug testing

A

Liquid Chromatography - Mass
Spectroscopy (LC-MS)

39
Q

chemicals that are
naturally found of produce within
an organism. Foreign, not expected
to present with the organism of
human and animals

A

XENOBIOTICS

ANACHEM (18 decks)

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