Module 3 Flashcards

Archaea

1
Q

features archaea have in common with bacteria

A

molecules in the cytoplasm (mRNA, tRNA, ribosomes, and DNA)
can have inclusion bodies - gas vacuoles, vesicles, etc
most have a single circular chromosome
lack a membrane-bound nucleus

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2
Q

features archaea have in common with eukaryotes

A

have a polymerase in common, they’re homologues
have histone proteins

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3
Q

how do histone proteins differ in archaea vs eukaryotes

A

eukaryotic histone proteins form an octamer, archaea: tetramer

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4
Q

definition: nucleosome

A

DNA wrapped around histone proteins

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5
Q

what keeps nucleosomes together

A

histone proteins are positively charged
DNA is negatively charged

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6
Q

what is typically in the cell envelope of archaea

A

cell membrane
cell wall
no outer membrane

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7
Q

what are some differences with archaea plasma membrane

A

the polar head groups are glycerol 1-phosphate instead of glycerol 3-phosphate (mirrored)
there is a phytanyl chain instead of fatty acid tails

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8
Q

what is the structure of a phytanyl chain

A

4 repeated isoprene units (a 5-carbon compound)

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9
Q

what is the benefit of the phytanyl chain being linked by an ether linkage as opposed to an ester one

A

beneficial for cells inhabiting high temperatures
brings additional stability

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10
Q

definition: biphytanyl chain

A

two sets of phytanyl chains covalently linked in the middle
stronger than those is a bilayer

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11
Q

when will the proportion of biphytanyl chains increase

A

in higher temperatures

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12
Q

what is a tetraether layer

A

another name for the monolayer that archaea will form

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13
Q

what are some differences in the archaea cell wall

A

has beta-1,3-glycosidic linkages
has NATs instead of NAMs
there are no D-amino acids present

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14
Q

describe: S-layer

A

surface layer/slime layer
protects against predation and viruses
mediates adhesion to existent biofilm

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15
Q

describe: cannulae

A

they are tubes made up of sugars and proteins
form complex networks
function is not well understood

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16
Q

compare archaea flagella to bacterial flagella

A

assembled from the base outwards
simpler
more solid
more narrow
made up of more than one type of protein

17
Q

what are the 2 major phyla of archaea

A

Euryarchaeota
Crenarchaeota

18
Q

what is the main feature of Crenarchaeota

A

they are extremophiles
temperature, pressure, acid, etc.

19
Q

having increased proportions of arginines and tyrosines is a feature of which archaea

A

thermophiles (and other extremophiles)

20
Q

what is a way that thermophiles increase their stability in terms of metabolism

A

they possess reverse DNA gyrase
increases the extent of DNA supercoiling

21
Q

what is the main feature of Thaumarchaeota

A

they are intermediate/lower temp loving (mesophilic)
many are found in the ocean

22
Q

where in the ocean are there more archaea than bacteria

A

lower down

23
Q

what is the main feature of Euryarchaeota

A

contains all known methanogens

24
Q

what are some features of halophiles

A

require very high salt concentrations
have high concentrations of potassium to combat high salinity

25
having increased proportions of genomic guanine and cytosine is a feature of which archaea
halophiles G and C pairs have 3 hydrogen bonds
26
what causes the red colour in halophiles
it is caused by a light-harvesting pigment, retinal produced in low-oxygen conditions helps supplement the high requirements for ATP
27
where are methanogens found
in environments that don't have any oxygen methanogens are strict anaerobes e.g. large intestines, sediments, wastewater sludge, rice paddies, landfills
28
what do many methanogens require and what do they produce
CO2 and H2 - parts of the end products of fermentation (+ acetic acid) they produce methane
29
what do methanogens use as an electron acceptor
CO2 H2 gets oxidized
30
what are some types of methanogens
hydrogenoclastic - take in H2 acetoclastic - take in acetate, CH3CO2 methylotrophic - use one-carbon compounds, like methanol (CH3OH)
31
what are some suitable electron acceptors of methane
oxygen, nitrate, or sulphate
32
what is the main feature of Nanoarchaeota
the only member is Nanoarchaeum equitans
33
what is Nanoarchaeum equitans found in association with
Ignicoccus hospitalis
34
what do Nanoarchaeum equitans lack
metabolic genes ability to make ATP, AA's, nucleotides ability to make lipids
35
Ignicoccus features
extended periplasm ATP synthases are housed in the outer membrane lots of vacuoles in the periplasm