module 1 - cells Flashcards
what are the 2 types of cells ?
prokaryotic
eukaryotic
what is a prokaryotic cell ?
- a cell which doesn’t have a nucleus or membrane bound organelles
- has smaller ribosomes
- genetic material is loose in cell
what is a eukaryotic cell ?
- has a distinct nucleus and membrane bound organelles
- has bigger ribosomes
what 3 organelles are found in plant not animal cells ?
permanent vacuole
cell wall
chloroplasts
what is the function of the nucleus ?
responsible for managing the cells genetic material
controls cell activity
what is the function of the nucleolus ?
where ribosomal rna is formed
where ribosome subunits are assembled
what is the function of the mitochondria ?
to produce energy through respiration
what is the function of the vesicles ?
have storage and transport roles:
transport materials inside the cell
what are the function of lysosomes ?
contain hydrolytic enzymes responsible for breaking down waste materials
what are lysosomes specialised forms of ?
vesicles
what is the function of the rough endoplasmic reticulum ?
has ribosomes bound to the surface
responsible for synthesis and transport of proteins
maintains different conditions from cell cytoplasm
separates synthesized proteins from cell cytoplasm
holds ribosomes in place
what is the function of the smooth endoplasmic reticulum ?
responsible for lipid and carbohydrate synthesis and storage
what is the function of the ribosomes ?
the site of protein synthesis
what is the function of the golgi apparatus ?
modifying and packaging proteins into vesicles
what is the function of the cytoskeleton ?
network of fibres necessary for the shape and stability of the cell
what is the centriole, how is it structured ?
- part of cytoskeleton
- composed of microtubules
- 2 associated centrioles form the centrosome
what is the function of the centrioles ?
involved in the assembly and organisation of spindle fibres in cell division
what is the function of the flagella ?
helps an organism in movement
sometimes used to detect changes in the environment
what are the 2 types of cilia ?
stationary
mobile
what is the function of stationary cilia ?
used for sensory organs
what is the function of mobile cilia ?
causes liquids/objects in the cell to move
what is the function of the vacuole ?
contains cell sap
helps with maintenance of turgor so contents of cell push against cell wall - causing the cell to stay rigid
what is the function of the chloroplasts ?
responsible for photosynthesis, contains chlorophyll
what cell organelles are only found in prokaryotic cells ?
nucleoid
plasmid
pili
capsule
what cell organelles are only found in eukaryotic cells ?
nucleus
mitochondria
chloroplasts
golgi
rough er
smooth er
what cell organelles are found in prokaryotic and eukaryotic cells ?
ribosomes
flagella
cell membrane
cell wall
cytoplasm
what are the 3 types of microscope ?
light
scanning electron microscope
transmission electron microscope
give features of electron microscopes ?
- large
- not portable
- vacuum needed
- complicated sample prep
- over 500 000 magnification
- resolution 0.5 nm
- specimens are dead
what is the magnification of an electron microscope ?
over 500 000
what is the resolution of the electron microscope ?
0.5 nm
give properties of the light microscope ?
small
easy to carry
no vacuum needed
easy sample prep
up to 2000 magnification
200 nm resolution
specimens living or dead
what is the magnification of a light microscope ?
x2000
what is the resolution of a light microscope ?
0.5 nm
give a disadvantage of the preparation process in electron microscopes ?
- time consuming
what is magnification ?
how many times bigger an image appears than it actually is in real life
what is resolution ?
the ability to distinguish 2 objects which are very close together
mm - mcm - nm convertion
mm x1000 mcm x1000 nm
how do you calculate magnification ?
image size / actual size
give properties of a scanning electron microscope ?
3 d image produced
high magnification
high resolution
black and white
give properties of a transmission electron microscope ?
high magnification
high resolution
2 d image produced
black and white
give properties of a light microscope ?
colour
low magnification
low resolution
2 d image
specimens - living or dead
what is a plasma membrane ?
all the membrane of cells
what is the function of the cell wall ?
strong flexible layer which surrounds some cell types
what is the function of a nuclear membrane ?
double layered membrane which encloses nucleus
what is a chromosome ?
structured of condensed dna in the form of chromatin
what is chromatin ?
uncondensed dna in a complex of histones
what is a microtubule ?
part of cytoskeleton, used for cell shape, cell movement, and cell division (mitosis)
what is a microfilament ?
solid strands made of protein actin, causes some cell movement
what is spindle apparatus ?
part of cytoskeleton, structure which separates sister chromatias during cell division
what is undulipodium ?
used for locomotion, moving fluids
what is a microscope ?
a tool used to magnify small objects
what is plasmodesma ?
microscopic channels which connect walls of plant cells and some algae cells to transport materials and for communication
do a flow chart showing how proteins are transported around a cell
- gene coding for protein transcribed into messenger RNA in nucleus
- Mrna levee through nuclear pores
- goes to rough ER
- ribosomes on rough ER translate mRNA and assemble insulin molecules
- pass to cisternae of RER
- vesicles containing protein are pinched off RER
- travels to golgi apparatus
- protein is packaged and processed
- modified protein molecules bud off golgi apparatus in vesicles
- vesicles fuse with plasma membrane
- exocytosis - plasma membrane opens to release protein molecules outside
what is the function of the cytoskeleton ?
- network of fibres present in cell
- holds organelles in place
- controls movement of cells and organelles
what makes up the cytoskeleton ?
microtubules
actin filaments
intermediate filaments
what is the structure of microtubules ?
- globular tubulin proteins polymerase to form tubes which form a scaffold like structure
what is the function of microtubules ?
- determines shape of cell
- acts as tracks for the movement of organelles e.g vesicles
- forms spindle fibres
what are actin filaments ?
contractile fibres formed from the protein actin
what is the function of actin filaments ?
- responsible for cell movement and cell contraction during cytokenisis
what are intermediate filaments ?
- fibres which give mechanical strength to cells to help maintain their integrity
what are dry mounts used for ?
- solid specimens
- hair, pollen dust, muscle tissue, plant tissue
how are dry mounts used ?
thin slices called sectioning
coverslip is placed on top of
what are wet mounts used for ?
wet specimens
aquatic samples, other living organisms
how are wet mounts made ?
suspended in water or immersion oil
coverslip is placed at angle
what are squash slides used for ?
soft specimens
root cells to look at cell division
how are squash cells used ?
wet mount squashed between slide and coverslip
what are smear slides used for ?
body fluid specimens
blood smears
how are smear slides made ?
edge of slide is used to smear the sample creating a thin even coating
a coverslip is placed on top of the
what are stains used for ?
creating visibility so you can see the internal structure of a specimen clearly
what is contrast ?
increased with a stain between the cell and environment or between cell organelles
what is sectioning ?
cutting a specimen into thin slices
how is a tem used
- thin specimen placed in vacuum
- electron gun produces a beam of electrons which pass through the specimen
in a tem how are darker parts of the 2-D image formed
- it is the parts of the specimen that have absorbed more electrons
how are the images in electron microscopes formed
by electromagnets
why is the electromagnet stored in a vacuum for microscopy
electrons are absorbed by air
how to sems work
the electrons bounce of the specimen in different directions depending on the contours of the specimen producing a 3-D image
capsule
prevents bacteria from drying out - slimy layer made of protein
how can microtubules be prevented
- respiratory inhibitor
microtubules - function
- involved in moving chromosomes to the equator to the poles of the cell during mitosis
ribosomes - structure
- not membrane bound
- made of rrna
- made of protein
structure onion root cells -
contain mitochondria
have cellulose cell walls
what shouldn’t you do in microscope drawings
- shade
- use a blunt pencil
- not include a title
- not include a scale bar
- make arrow heads cross
- add arrow heads
how does the mitochondria in the goblet cells help it to carry out its function
- provides lots of ATP
- modifies packages proteins
- secretion of mucus
means of cell division - yeast
- budding