Mod 1 Flashcards
In vivo
Events that occur inside the body
What factors affect microbial growth
Availability of nutrition, moisture, temperatures, PH, osmotic pressure, barometric pressure and composition of the atmosphere
Nutrients
Various chemical compounds that organisms use to sustain life- gases and other elements
Moisture
Cells consist of anywhere between 70% and 95% water
Microorganism that a are commonly on the body are know as what?
Normal flora
What is an optimum growth temperature?
Where the organism grow best
Minimum growth temperature does what?
Ceases the growth
Maximum growth temperature does what?
Causes it to die
What grows best in high temperatures? (Up to 113 degrees C)
Thermophiles
What grows best in normal body temperature? (Plants and animals)
Mesophiles
What grow best in cold temperatures? (Ocean water)
Psychrophiles
A group of psychrophiles known as psychrotrophs grow best where?
In the refrigerator at 4 degrees C
What microbes live in the human stomach?
Acidophiles (Ph 2-5)
Alkaliphiles live where?
Inside the intestines (ph > 8.5)
What is osmotic pressure?
Pressure exerted on a cell membrane by solutions both inside and outside the cell
What is it called when the concentration of solution in the environment outside the cell are greater then the concentration inside the cell?
Hypertonic
What is osmosis?
Is the movement of solvent through a permeable membrane from a solution having lower concentration of solute to a solution having higher concentration of solute
What is the shrinkage of a cell due to loss of water called?
Crenated
What is it called when the concentration of solute outside the cell are less than the concentration inside the cell?
Hypotonic
What is it called when the concentration of solute are equal on the inside and outside?
Isotonic
What microbes prefer the same atmosphere as humans?
Aerobes (20%-21% oxygen and 78%-79% nitrogen)
What microbes require reduced concentrations of O2?
Microaerphiles 5% O2
What are killed by oxygen?
Obligate anaerobes
What does bacterial growth refer to?
Increase in numbers
What is binary fission?
The process of one cell dividing into two cells
What is generation time?
The time it take one cell to become two cells
Under ideal conditions what is the cell generation time of E. Coli, V. cholerae, Staphylococcus and streptococcus?
20 minutes
Under ideal conditions what is the generation time for pseudomonas and clostridium?
10 minutes
Under ideal conditions what is the generation time for mycobacterium and tuberculosis?
18- 24 hours
artificial or synthetic media
Do not occur naturally