Missed Practice Questions Flashcards
Secreted Proteins
A) Begin with a signal peptide
B) Are translocated into the ER during translation
C) can be glycosylated
D) all
E) None
D) All of the above
Antibiotics are added to a solid culture medium for plating bacteria transformed with recombinant plasmids to:
prevent nontransformed bacteria from yielding colonies.
Phorphoramidite synthesis of DNA uses protecting groups to:
prevent the addition of multiple nucleotides during each cycle.
Reverse transcriptase
A) is necessary for the life cycle of retroviruses
B) catalyzes DNA polymerization using an RNA template
C) requires priming by a short oligonucleotide
D) All of the above
E) none of the above
D) all of the above
What is a common type of protein secondary structure?
alpha helix
What are some difference between the cytoplasm and ER that are important to protein folding and structure?
1) Oxidizing Environment
2) Molecular Chaperones
3) Foldases
4) Post-Translational glycosylation
What is the primary force driving the rapid compaction of unfolded proteins?
The hydrophobic effect
What are organisms living in high temperature environments?
thermophiles
Define Vector:
a vector is a tool for introducing a gene into a cell in a functional form that can propogate
Define Inclusion Body:
microscopic aggregates of protein inside the cell
How do you determine single step coupling efficiency?
YIELD=(efficiency)^(n-1)
where n = oligonucleotide length
What are some forces that stabilize protein structure?
1) Pi stacking
2) H-bonding
3) Ionic Bonding
4) Hydrophobic Bonding
5) Covalent Bonding (Disulfide Bonds)
6) Van der Waals
How do you find the correct number of product molecules from PCR?
(starting molecules)(2)^(n-2), where n = #cycles
then use avagadro’s number to conver to moles.
then use 1bp=660g/mol to get mass
What is one reason for artificially synthesizing a gene?
The gene is eukaryotic and contains both exons and introns
What does the melting temperature of DNA depend on?
1) sequence
2) length
3) solution cation concentration
T/F: DNA ligase catalyzes hybridization of complementary sticky ends
F (hybridization in creating double strands, ligating is just joining end to end)