Miss Lee - Micoscopes

Question 1

Features of a light microscope

Answer 1

Expensive to buy and operate. The radiation used is light. It is small and portable. The magnification maximum is times 2000. The maximum resolution is 200 nm. The images are produced is a natural colour is seen or a stain. There is no distortion to the sample. It does not require a vacuum

Question 2

Features of a scanning electron microscope

Answer 2

It is expensive to buy and operate. The radiation used is an electron. It is large and needs to be installed. The maximum magnification is times 100,000. The maximum resolution is 3 to 10 nm. The image produced is black and white and is coloured Digitally. The sample preparation is complex and often distorts the image. And there is a vacuum needed

Question 3

Features of a transmission electron microscope

Answer 3

It is expensive to buy and operate. Radiation used is electron. Size is very large and requires installation. Maximum magnification is times 500,000. Maximum resolution is .5 nm. Type of image produced is it black and white and is coloured digitally. Sample preparation is complex and often distort the image. And a vacuum is needed

Question 4

Advantages of transmission electron microscope is over scanning electron microscope

Answer 4

A transmission electron microscope allows you to serve detail as small as atoms you to high magnification and resolution

Question 5

Advantages of scanning electron microscope over transmission electron microscope

Answer 5

Large amounts of sample to be observed at one time

Question 6

Define magnification

Answer 6

Magnification is how large the image is (under a microscope) compare to the actual size

Question 7

Define resolution

Answer 7

Resolution is the ability to distinguish between two different destructive points on an image

Question 8

What is a scale bar formula

Answer 8

Measured length of scale bars over actual length of scale bar

Question 9

I am Formula

Answer 9

Image over magnification x actual size

Question 10

Eyepiece graticule what is it

Answer 10

And eyepiece graticule is it a device used to measure the size of your specimen under a microscope. E.g. a ruler for the microscope.

Question 11

How is an eyepiece graticule fitted

Answer 11

Can I please graticule is fitted by sliding onto the eyepiece lens

Question 12

What is a stage micrometre

Answer 12

A stage micrometre is a glass slide paste onto the stage. It’s an accurate scale usually has 100 divisions This leads to each division being 0.01 mm in length and are used to work out the value of each die version onto the eyepiece graticule at a particular magnification

Question 13

Stages to calibrate the eyepiece graticule

Answer 13

1. Place the IP scared you’re in the eyepiece, and placed the stage micro meter on the stage.
2. The line 0 point of the Eyepiece graticule with the zero point of the stage micrometre.
3. Scanning along with a scale is exactly aligned again.
4. Count how many IPC units are equal to the stage micro meter units e.g. 20 EPu equals 10 stage micrometer units (10×10 = 100um)
5. From what you’ve calculated in step four you can work out 1EQU division