midterm Flashcards

1
Q

difference between covalent and ionic bonds

A

-covalent: two atoms that have the same charge (nonmetal and nonmental), share elctrons
-ionic: two atoms that are differenty charged (metal and nonmetal), steal electrons

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2
Q

how do antacids work (based on your knowledge of buffers and the pH system)?

A

-work like buffers to absorb the excess H+ ions in order to bring u the pH and level out the acidity to make the stomach in normal pH range
-low pH = high acidity = excess H+
-high pH = high basity = excess OH -

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3
Q

six properties of water

A

-solvency
-cohesion and adhesion
-high surface tension
-high heat capacity
-high heat of vaporization
-varying density

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4
Q

explain how solvency (in water) is important to life

A

-Due to polarity and H-bonding, water dissolves many substances
-universal solvent
-important to majority of organisms on earth

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5
Q

explain how cohesion and adhesion (in water) is important to life

A

-Allows water to be excellent transport system both inside and outside of living organisms
-Contributes to water transport in plants

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6
Q

explain how high surface tension (in water) is important to life

A

-allows certain organisms to float, move, and even live on the water’s surface

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7
Q

explain how high heat capacity (in water) is important to life

A

-The many hydrogen bonds linking water molecules allow water to absorb heat without greatly changing its temperature.
-Temperature of water rises and falls slowly
-Helps humans/organisms composed of water be able to maintain body temp

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8
Q

explain how high heat of vaporization (in water) is important to life

A

-Takes a great deal of energy to break H bonds for evaporation.
-Heat is dispelled as water evaporates
-helps water reserves stay and not evaporate totally in the sky

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9
Q

explain how varying density (in water) is important to life

A

-Unlike other substances, water expands as it freezes.
-Ice floats rather than sinks.
-It makes life possible in water.
-Ice acts as an insulator.

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10
Q

difference between organic and inorganic molecules

A

-organic must have carbon and hydrogen

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11
Q

basic characterisitcs of all cells

A

-plasma membrane
-cytoplasm
-cytoskeleton
-ribocomes
-genetic information
-can have cell wall

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12
Q

differences between prokaryotes and eukaryotes

A

-pro: no membrane bound organelles or nucleus (do have cytoplasm, ribosome, plasma membrane, nucleiod instead of nucleus)
-eu: nucleus, all membrane bound organelles

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13
Q

how might knowing the structure of an active site of an enzyme allow you to build a drug to regulate a metabolic pathway?

A

-specifically shape the molecule to compete with substrate for enzyme’s active site and bond to it
-acts like feedback inhibition
-regulates how much product is made by restricting substrates from bonding to active site and therefore reaction from occuring

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14
Q

how do enzymes speed up chemical reactions?

A

-by bringing reactants (substrates) together so they can bind, forcing them to react with each other
-by lowering activation energy required to start a reaction
-instead of relying on molecules to naturally and randomly bump into each other we rely on enzymes to force the reaction to happen

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15
Q

how are cellular respiration and photosynthesis connected?

A

-molecules are cycled through both
-photosynthesis is chlorplasts taking water, carbon, and making them into carbohydrates using colar energy
-cell respiration is mitchondria breakidng down carbs to create energy in the form of ATP

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16
Q

why do broad thin leaves provide an advantage for photosynthesis?

A

-broad thin leaves allow for more surface area to be exposed to teh sun to absorb solar energy
-thinnes allows their voluem to be limited so the process of transporting molecules is quicker

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17
Q

why is water needed in the light reactions?

A

-after energized electrons are passed of to the electron acceptor they need to be replaced
-H2O is broken down to provie these replacement elecrons
-H+ ions are also produced from photolysis are needed to reduce NADP+ and make it accept H+ ions to create NADPH

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18
Q

List the inputs and outputs of the preparatory reaction

A

-2 pyruvate produced form glucose when it is brroken down in glycolysis
-chagned acetyl grup to bind to co-enzyme A to make acetyl Co-A (prepping them for citric acid cycle)
-breaks down pyruvate into CO2 to eventually bond it to acetyl CoA in the citric acid cycle

19
Q

What are the four stages of cellular respiration?

A

-glycolysis
-prepatory reactions
-citric acid cycle
-electron transport cahin

20
Q

list inputs and outputs of citric acid cycle

A

-inputs: 2 acetyl co-a, 6 NAD+, 2 FAD, 2 ADP + 2P
-outputs: 4 CO2, 6 NADH, 2 FADH2, 2 ATP

21
Q

list inputs and outputs of electron transport chain

A

-inputs: electrons from NADH and FADH2, H+, and O2, 6 carbon molecule made from 2 carbon acetyl group and 4 carbon molecule
-outputs: H2O, ATP, NADH, FADH2

22
Q

Distinguish between a sister chromatid and a chromosome.

A

-sister chromatids: two halves connected by a centromere to form a chromosome (I shaped)
-chromosomes: made up of two sister chromatids connected by a centromere (X shaped)

23
Q

what are the phases of interphase?

A

-G1
-S
-G2

24
Q

what happens in G1 of interphase?

A

-organelles double
-cell collect materials needed for DNA synthesis
-makes final decision whether to divide or not

25
Q

what happens in G0 of interphase?

A

-happens if cell makes decision not to divide
-continues normal funtions w/o dividing

26
Q

what happens in S phase of interphase?

A

-DNA synthesis occurs (DNA doubled)

27
Q

what happens in G2 of interphase?

A

-checks over DNA to verify its replicated
-repairs any damage to DNA
-continues growing
-gathers any materials needed for cell reproduction

28
Q

Meiosis creates a very large amount of genetic variability within a population. Why might this be advantageous?

A

-we need to keep the gene pool diverse so we aren’t susceptible to disease and do not die out as a population

29
Q

Explain the significance of checkpoints in the cell cycle.

A

-chekpoints help the cell make sure everything is right before moving forward with the cell cycle
-make sure there are no major deformities in the DNA so they won’t be passed down to other cells
-if they were this could create cancer
0if the checkpoints are not passed the cell will go through apoptosis (of G0 if it is ghe G1 checkpoint)

30
Q

Why is mRNA modified following transcription?

A

-to make it more stable
–these processes provide stability and get rid of unnecessary info (introns)

31
Q

Describe the process of transcription.

A

mRNA is formed by:
-RNA polymerase binding to a promoter
-opening up DNA helix just in front of it
-complementary base pairing RNA nucleotides
mRNA is then processed in the nucleus by:
-adding a cap of altered guanine nucleotide
-adding a poly-A tail to the 3’ end
-splicing the extrons together after removing the introns

32
Q

Describe the three steps of translation.

A

-initiation: mRNA attaches to the small ribosomal subunit, large subunit joins to them
-elongation: peptide is synthesized one amino acid at a time (tRNA brings amino acids that mRNA codes for in its codons)
-termination: one of 3 stop codons is reached (UAA, UAG, UGA), this triggers MRAN to be relased from the ribosomal subunits and a full polypeptide chain is thre product

33
Q

What is the difference between translocation and inversion?

A

-inversion: occurs when a segment breaks off and reattaches within the same chromosome, but in reverse orientation
-translocation: A genetic change in which a piece of one chromosome breaks off and attaches to another chromosome.

34
Q

Explain why a cell would use active transport.

A

-if it needed more of a molcuel that it already had a lot of (going against concentration gradient)

35
Q

Distinguish some advantages CAM plants have over C4 plants

A

-CAM plants are able to minimize water loss better than C4 plants
-they do it by deciding closing stomata during the day
-CAM are more widespread and compete well with C4 and C3 in arid environments
-C4 adapted for dry and don’t do as well in the cold
-CAM can adapt to more environments

36
Q

Summarize the roles of preparatory reaction, citric acid cycle, and electron transport chain in cellular respiration. Include how much ATP is made at each stage.

A

-prep rxns produce substrate that enters citric acid cycle (no atp)
-in citric acid cycle substrate level atp synthase occurs –> 2 ATP, completes the breaking down of glucose into carbon dioxide, produces NADH and FADH2 which help in ETC
-in ETC NADH and FADH2 accept electrons, energy from them pumps H+ from matrix to intermembrane space (creates gradient), energy used to make ATP (34 ATP)

37
Q

Identify the phases of mitosis, and explain what happens to the chromosomes in each phase.

A

-prophase: chromatin condenses into chromosomes and nuclear envelope breaks down
-metaphase: chromosomes meet in the middle of the cell (spindle equator)
-telophase: spindle fibers attach to chromosomes
-anaphase: chromosomes are pulled to opposite poles
-telophase and cytokinesis: cytoplasm breaks and new nucleus forms creating two new cells, chromosomes become chromatin again

38
Q

Describe the events that are involved in the expression of the information contained in the DNA to a functional protein.

A

-transcription and translation
-mRNA is synthesized from DNA, taking the information out of the nucleus to ribosomes
-polypeptide chain is synthesized from mRNA
-mRNA directs the info from codons
-tRNA gets amino acids that are coded for
-reaches stop codon and protein is released

39
Q

Describe the process of DNA replication, including the functions of the major enzymes involved.

A

-helicase unqinds the DNA separating the double helix at the nitrgen bases by breaking hydrogen bonds
-creates two old strands that serve as templates for new
-nucleotides are complementary base paired to old strandes
-DNA polymerase completes teh DNA strand
-synthesized in 5’ 3’ direction
-DNA ligase will fix any gaps in the stand
-semiconservatiev bc uses old strands as templates

40
Q

Compare and contrast the structure of DNA and RNA.

A

-DNA: double helix strucutre, bases of A,T,C, and G, made of deoxyribose sugar
-RNA: single strange, bases of A, C, G, and U, made of ribose sugar

41
Q

Explain why a point mutation may be silent, but frameshift mutations rarely are.

A

-point: change in single DNA nucleotide, Results can be minor or severe bc some different codons will code for the same amino acids, and even if it is differnet is only affects one amino acid
-frameshift: All downstream codons affected

42
Q

What did Erwin Chargaff discover about DNA?

A

-made chargaff’s rules
-knew that DNA contained 4 types of nitrogen bases
-examined DNA from many species
-said amount of A, T, C, and G varies from species to species
-amnt of A always equals amnt of T and amnt of C always equals amnt of G

43
Q

What did James Watson and Francis Crick discover about DNA?

A

-watson and crick set out in 1951 to bring all the data they had on DNA and build a model
-called watson and crick model
-also suggested how replication works with their model