Mid 1

Question 1

false positive

Answer 1

measurable result where there shouldnt be any

Question 2

false negative

Answer 2

no result but there should be positive control

Question 3

sensitivity

Answer 3

minimum amont of needd to record positive resuly

Question 4

specificity

Answer 4

positive result only comes from a truly positive sample

Question 5

Quality of microscope

Answer 5

• thickness of section
• lighting angle
• quality
• staining

Question 6

R (distance we can see things touch)

Answer 6

0.61 lambda /N( numerical aperture of lens) A

Question 7

resolution

Answer 7

affects detail. more detail= more resolution

Question 8

Magnification

Answer 8

affected by size of image, bigger image needs more magnification

Question 9

Improve Resolution?

Answer 9

wider lens
smaller distance (R)
immersion oil
smaller wavelength

Question 10

-NA

Answer 10

nSintheta

Question 11

spectroscopy

Answer 11

study of absorption and emission of radiation

Question 12

Photometry

Answer 12

measurement of intensity of radiation

Question 13

Wavelength

Answer 13

higher energy is shorter wavength blue

lower energy is longer wavength red

Question 14

Transmittance

Answer 14

proportion of light that the sample allows to pass

Question 15

absorbance

Answer 15

amount of light sample blocks A=log(1/T)

Question 16

Standard Curve

Answer 16

series of known samples used to generate comparison data

Question 17

Absorbance Spectrum

Answer 17

Wavelength vs relevance absorbance

Question 18

Boer Lambert Law

Answer 18

Absorbance=e(molar absorbance)L(path length)C(concentration)

Question 19

Strepto

Answer 19

chains

Question 20

Staphyl

Answer 20

clusters

Question 21

Negative gram stains

Answer 21

pink

Question 22

positive gram stains

Answer 22

purple

Question 23

Agar

Answer 23

carbohydrate from cell wall of red algae. melts at 85 degrees, solidifies at 40

Question 24

Endospore Stain

Answer 24

Primary -Malachite Green

counterstain - Safranin

Question 25

Acid-fast

Answer 25

primary- carbol-fuschin

counterstain - methylene blue

Question 26

Negative stain

Answer 26

counterstain- crystal violet

Question 27

Gram stain

Answer 27

primary-

counterstain- safranin

Question 28

competitive inhibition

Answer 28

vmax, km increases
A compound binds the active site
and blocks substrate binding, intercepts on y axis

Question 29

uncompetitive inhibition

Answer 29

A compound binds the ES complex and slows

the transition to EP, but does not bind E alone.. vmax and km decreases