Mid 1 Flashcards
false positive
measurable result where there shouldnt be any
false negative
no result but there should be positive control
sensitivity
minimum amont of needd to record positive resuly
specificity
positive result only comes from a truly positive sample
Quality of microscope
- thickness of section
- lighting angle
- quality
- staining
R (distance we can see things touch)
0.61 lambda /N( numerical aperture of lens) A
resolution
affects detail. more detail= more resolution
Magnification
affected by size of image, bigger image needs more magnification
Improve Resolution?
wider lens
smaller distance (R)
immersion oil
smaller wavelength
-NA
nSintheta
spectroscopy
study of absorption and emission of radiation
Photometry
measurement of intensity of radiation
Wavelength
higher energy is shorter wavength blue
lower energy is longer wavength red
Transmittance
proportion of light that the sample allows to pass
absorbance
amount of light sample blocks A=log(1/T)
Standard Curve
series of known samples used to generate comparison data
Absorbance Spectrum
Wavelength vs relevance absorbance
Boer Lambert Law
Absorbance=e(molar absorbance)L(path length)C(concentration)
Strepto
chains
Staphyl
clusters
Negative gram stains
pink
positive gram stains
purple
Agar
carbohydrate from cell wall of red algae. melts at 85 degrees, solidifies at 40
Endospore Stain
Primary -Malachite Green
counterstain - Safranin
Acid-fast
primary- carbol-fuschin
counterstain - methylene blue
Negative stain
counterstain- crystal violet
Gram stain
primary-
counterstain- safranin
competitive inhibition
vmax, km increases
A compound binds the active site
and blocks substrate binding, intercepts on y axis
uncompetitive inhibition
A compound binds the ES complex and slows
the transition to EP, but does not bind E alone.. vmax and km decreases
