L17

Question 1

What inhibits polymerization in the SDS-PAGE procedure

Answer 1

oxygen inhibits polymerization

Question 2

Coomassie blue staining

Answer 2

not dependent on sensitivity, simple

Question 3

Silver nitrate

Answer 3

used on low quantity and rare proteins. time consuming. higher sensitivity

Question 4

Fluorescent dye staining

Answer 4

sensitive and requires specialized equipment

mid range

Question 5

What are the movements of Cl, glycine, and protein in Gel portion

Answer 5

Cl- very small and charged. fastest
Glycine- small and weakly charged, slowest
Protein- quite large, highly charged. faster than glycine

Question 6

What are the movements of Cl, glycine, and protein in running portion

Answer 6

Cl- fast
glycine- high negative charge, very fast
protein- same charge, thicker gel makes small proteins faster

Question 7

What is relative mobility

Answer 7

how far protein band goes compared to something fast (dye)

Question 8

What is the relationship between mobility and size in acrylamide concentrations

Answer 8

mobility increases EXPONENTIALLY as size decreases