L18

Question 1

What is Urea denaturing gels

Answer 1

• procedure to separate like size proteins.
• hydrogen bonding with urea inhibits formation of secondary structure
• protein’s mobility are affected by size and charge

Question 2

What are native (non-denaturing) gels

Answer 2

• mobility depends on size, charge, and conformation
• charge depends on the pH of the buffer used
• gels run slower than SDS-Page

Question 3

What are ampholytes

Answer 3

molecules that can buffer at their pI. allows gel to maintains pH gradient.

Question 4

What are the varieties of antibodies present

Answer 4

IgG- main  
IgM- first
IgA- secreted
IgD- dispensible
IgE- allergies