L20

Question 1

Western Blotting (Immunoblotting)

Answer 1

using antibodies to look for a protein of interest.
Gel transferred to a membrane with high protein affinity (capillary transfer, electrophoretic transfer).
Blotting, Blocking, and antibody detection

Question 2

Isotope labeling

Answer 2

125I or 35S are attached to protein and as the isotope decays the electrons expose X-ray film layered over the blot.

Question 3

Enzyme Labeling

Answer 3

enzyme catalyzes a reaction to give a colored substance or chemiluminescence. Common enzymes like horseradish peroxidase (HRP)
or alkaline phosphatase (AP)

Question 4

Fluorescent Labeling

Answer 4

dye attached that absorbs one color of light and gives off another

Question 5

RPE65

Answer 5

gene involved in recycling vitamin A.

Question 6

Consequences of lack of RPE65

Answer 6

blindness