L16

Question 1

Limitation of size exclusion chromatography

Answer 1

• Turbulence from layering sample over gel
• Diffusion during travel
• friction differences along the glass tube wall

Question 2

What is the relationship between the charge of two molecule and their speed traveling down the column

Answer 2

same charge - moves fastest
neutral - slow
opposite charge - slowest

Question 3

What amount of charge travels faster down the column

Answer 3

the greater the charge on a protein the slower it will travel

Question 4

Affinity Chromotography

Answer 4

specific interaction between protein and trap (substrate|enzyme) that holds it in the column.

Question 5

Order of Specificity

Answer 5

• Affinity chromatography
• Ion exchange chromatography
• Size exclusion chromatography

Question 6

Electrophoresis

Answer 6

migration of ions in an electric field

Question 7

Protein Gel Electrophoresis

Answer 7

Gel used to slow down movement and prevent diffusion of proteins and create separation.

Question 8

SDS-PAGE

Answer 8

detergent that denatures proteins and give them a negative charge

Question 9

Acrylamide

Answer 9

plastic monomer (toxic, carcinogenic, teratogenic), used industrially and can be chained together.