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OCR Gateway Biology > Microscopy > Flashcards

Microscopy Flashcards

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1
Q

Magnification calculation

A

Magnification = image / actual

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2
Q

Magnification definition

A

Number of times larger an image is, compared with the actual size of the object

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3
Q

How many millimetres is 1 metre

A

1000

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4
Q

How many micrometers is 1 millimetre

A

1000

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5
Q

How many nanometers is 1 micrometer

A

1000

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6
Q

Resolution and magnification definition

A

-resolution
How much detail you can see , or if you can see 2 points that are really close together
-magnification
How many times bigger the image is that the actual object

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7
Q

Why do we use electron microscopes?

A

Better resolution and magnification

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8
Q

Radiation used in light microscopes

A

Light rays

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9
Q

Radiation used in electron microscopes

A

Electron beam

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10
Q

How to focus a light microscope

A

Lens

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11
Q

How to focus electron microscopes

A

Magnets

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12
Q

What are the different types of electron microscope?

A
  • Scanning electron microscope (SEM)

- Transmission electron microscope (TEM)

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13
Q

Biological matter studied by a light microscope

A

Living or dead

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14
Q

Biological matter studied by an electron microscope

A

Dead

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15
Q

Size of light microscope

A

Small and portable

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16
Q

Size of electron microscope

A

Very large and static

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17
Q

Ease of use for light microscope

A

Very easy

18
Q

Ease of use for electron microscope

A

Skill and training required

19
Q

Cost of light microscope

A

Relatively cheap

20
Q

Cost of electron microscope

A

Very expensive

21
Q

Can light microscopes use samples in a vacuum?

A

No

22
Q

Can electron microscopes use samples in a vacuum?

A

Yes

23
Q

Type of image from a light microscope

A

2D

24
Q

Type of image from TEM

A

2D (cross section)

25
Q

Type of image from SEM

A

3D

26
Q

Magnification of light microscope

A

X1500

27
Q

Magnification of TEM

A

X500 000

28
Q

Magnification of SEM

A

X100 000

29
Q

Why is having the highest magnification not always better? (2)

A
  • won’t focus properly

- you can see limited number of cells

30
Q

Resolving power of a light microscope

A

200nm

31
Q

Resolving power of a TEM

A

0.2nm

32
Q

Resolving power of a SEM

A

10nm

33
Q

How does a TEM work?

A

-send electrons through through a specimen

34
Q

How does a SEM work?

A

-bounce electrons off the surface of an object

35
Q

Resolving power of a light microscope

A

200nm

36
Q

Resolving power of a TEM

A

0.2nm

37
Q

Resolving power of a SEM

A

10nm

38
Q

How does a TEM work?

A

-send electrons through through a specimen

39
Q

How does a SEM work?

A

-bounce electrons off the surface of an object

40
Q

How to work out total magnification

A

Eyepiece lens x objective lens

OCR Gateway Biology (13 decks)

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