Microscopy

Question 1

resolution

Answer 1

ability of a microscope to distinguish between two objects as being separate

Question 2

what does higher magnification do

Answer 2

increases resolution

Question 3

what does higher contrast do

Answer 3

gives more detail but doesn’t increase the resolution

Question 4

light microscopy: reflected light

Answer 4

lighting from above creates the ability to see bigger sizes of cells

Question 5

Light microscopy: transmitted light

Answer 5

lighting from underneath

Question 6

contrast enhancing methods of transmitted light in light microscopy

Answer 6

darkfield, phase contrast, differential interference contrast

Question 7

light microscopy: florescence

Answer 7

exciting of electron to emit a photon of longer wavelengths

Question 8

confocal flourescence

Answer 8

increases contrast by removing light

Question 9

electron microscopy benefits and drawbacks

Answer 9

gives higher resolution
no colors, specimens must be dead

Question 10

electron microscopy: transmission

Answer 10

appears in black and white, lighter where the electrons passed through to hit the camera

Question 11

electron microscopy: scanning

Answer 11

no sectioning needed but coat the specimen in a heavy metal, often used at lower magnifications

Question 12

Darkfield

Answer 12

uses a light sample at an angle

Question 13

Phase contrast

Answer 13

gives higher detailed images from phase shifts in lighting

Question 14

Differential interference contrast (DIC)

Answer 14

gives a pseudo 3D appearance