microscopy

Question 1

Four types of light microscopy

Answer 1

-bright field light microscopy
-phase contrast
-normarski differential intereference
-Dark field microscopy

Question 2

Confocal laser scanning microscopy process

Answer 2

A wide range of focused laser beam light is used
the specimen is scanned
the detetcor measure the illumination
examination of cells in live tissues
3D image

Question 3

advantages of confocal laser microscopy

Answer 3

• Live cell imaging
• Real time imaging
• Multicolor imaging
• High-resolution and less background in thick tissues
• 3-D imaging

Question 4

Electron Microscopy

Answer 4

Higher resolution than light microscopy since
electrons have very short wavelengths compared to
visible light

Question 5

TEM Microscopy

Answer 5

Very thin sections are made using a microtome
* Images appear as black objects on white
background
* Light regions are where electrons passed
through the sample and hit the camera
* Dark regions represent areas where electrons
did not pass through to hit the camera

Question 6

SEM Microscopy

Answer 6

No sectioning is needed, just coat the sample with a heavy metal (usually gold)
* The 3D contours of the surface are visualized by scanning an electron beam across the specimen