Microscopy Flashcards
What is a microscope
It is used to see things the naked eye can’t see
What are the two types of microscope
Light and electron microscope
Why is an electron microscope better than light microscope
Because you can see 3d,higher resolution,higer magnification in an electron microscope however in a light microscope, it is easer to carry ,lower resolution,lower magnification,you can see life processes and watch cell division
What is magnification
Is how much bigger the image is then the actual specimen
What is resolution
The ability to distinguish two points
What the the equation for the total magnification
Eye piece lens x objective lens magnification
What is the objective lens
Three or four lenses with different magnification
What is the stage
The platform that holds the observation
What is the slide
The thin piece of glass that holds the specimen
What is the lamp
It illuminates the specimen
What is the base
Supports the microscope
What is the arms
Supports and connects the eye piece lens to the base
List the steps to prepare and view the plant cell
- Take a thin layer of onion skin
2,click it and hold it in place
3.add iodine to it
4.place a cover slip gently to prevent bubbles - Place the slide on the stage
6.click it to hold it in place
7.use the lowest power to allow you to have a wider view
8.use the coarse focus knob to move the stage up - Be carefully not to crush the slide
10.use the fine focus knob to make your image clearer
11.change the objective lens to the highest power to have a bigger and clearer image
Why do you stain the thin layer of onion skin when preparing a viewing a plant cell
To allow you to see the parts of the cell properly
10,000 micro meters are equal to what
1 cm
