Microscopes Flashcards
What are the different types of light microscope and its characteristic?
bright field, simple, compound, stereo or comparison
What are the different types of electron microscope?
Transmission electron (TEM), Scanning electron (SEM), Scanning transmission electron (STEM) and reflection electron (REM)
What are the different types of scanning tunnelling microscopes?
Photon scanning (PSTM), Scanning tunnelling potentiometry (STP), Atomic force (AFM) and Spin polarised scanning tunnelling (SPSTM)
What is a compound light microscope used for?
view things up close with detail
What is a comparison microscope used for?
Compare 2 objects
What is an electron microscope used for?
Focuses beam of electrons, higher magnification, higher resolution, only dead or inorganic species
What is a Transmission electron microscope used for?
2d, black and white images
What is a Scanning electron microscope used for?
3d images
What is a Scanning tunnelling microscope used for?
atomic level, changes in current to create image
What are the main parts of a microscope and what are they used for?
Light source (illuminate specimen, can be adjusted), Condenser (to focus light on specimen), Stage (to mount specimen on), Objective lens (to enlarge image) and eyepiece (to view image)
Define magnification
Number of times length, breadth or diameter of object is multiplied by
Define resolution
Smallest distance between 2 points in an object to be considered seperate and distinct structures
Define contrast
Differences in colour that make details stand out
Define resolving power
ability of lens to gather light and resolution of objective
Define numerical aperture
ratio of diameter of lens to focal length
