Microscope Basics Flashcards

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1
Q

what is the ocular lens

A

the eyepiece

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2
Q

what is the body tube

A

the thing connecting the eyepiece to the rest of the microscope

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3
Q

what is the nosepiece

A

the thing holding all the objectives, right under the body tube

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4
Q

what are the objectives

A

the 3 different lenses to magnify the specimen, either red, yellow, or blue

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5
Q

what is the arm

A

the steel bar connecting the objectives with the stage, where you are supposed to carry the microscope

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6
Q

what is the stage

A

the platform that holds the slide, can be raised and lowered

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7
Q

what are the stage clips

A

the two clips on either side of the stage that hold the slide in place

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8
Q

what is the diaphragm

A

the filter below the stage that allows you to control how much light can go into the specimen

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9
Q

what is the coarse adjustment

A

the knob on most but not all microscopes that moves the stage up and down rapidly

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10
Q

what is the fine adjustment

A

the knob on most but not all microscopes that moves the stage up and down slowly to pick out more details

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11
Q

what is the light

A

the light source that comes from under the slide or at an angle from the top of the slide to view the slide easily

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12
Q

what is the base

A

the heavy bottom of the microscope, you hold the base whenever you pick the microscope up

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13
Q

how do you calculate the power of magnification

A

multiply the power of the ocular lens by the power of the objective

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14
Q

what is the smallest objective power and color

A

the smallest objective is red. its objective power is 4. since the eyepiece power is 10, the red objective must have a 40x power because 4x10=40

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15
Q

what is the medium objective power and color

A

the medium objective is yellow. its objective power is 10. since the eyepiece power is 10, the red objective must have a 100x power because 10x10=100

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16
Q

what is the large objective power and color

A

the large objective is blue. its objective power is 40. since the eyepiece power is 10, the red objective must have a 400x power because 40x10=400

17
Q

what happens to the view of the image if you increase the magnification

A

the image will become more detailed but we will see less of the image

18
Q

why can’t you use the largest objective for some slides

A

sometimes there is not enough room and you could break the slide

19
Q

how do you get a wet mount slide in 5 steps

A

1) get a slide and coverslip
2) place a drop of water on top of the specimen in the middle of the slide
3) place the edge of the coverslip on one side of the specimen
4) slowly lower the cover slip on the water
5) place the slide on the stage and begin viewing

20
Q

why do you make a wet mount slide

A

to safely stick a specimen to the slide so its easy to view