Microscope Flashcards

1
Q

is the ability of a microscope to produce an image of an object at a scale larger (or even smaller) than its actual size. it also serves a useful purpose only when it is possible to see more details of an object in the image than when observing the object with the unaided

A

Magnification

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2
Q

is used to describe the ability of a microscope to distinguish details of a specimen or sample. In other words, the minimum distance between 2 distinct points of a specimen where they can still be seen by the observer or microscope camera as separate entities.

A

resolution

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3
Q

is the diameter of the viewing field measured at an intermediate plane of angle. To put it simply, it’s the diameter of the circular area you see when you look through the eyepiece of the microscope.

A

field of view

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4
Q

As the magnification increases, the field of view?

A

decreases

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5
Q

STRUCTURAL PARTS OF THE MICROSCOPE:

A
  1. HEAD
  2. ARM
  3. BASE
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6
Q

is a cylindrical metallic tube that holds the eyepiece lens at one end and connects to the nose piece at other end.

A

head

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7
Q

head is also called a?

A

body tube or eyepiece tube

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8
Q

It connects the eyepiece lens to the objective lens.

A

Head

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9
Q

What type of microscope that the head are adjustable so that the viewer can adjust the eyepiece for maximum visualization.

A

binocular microscopes

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10
Q

This is the part connecting the base to the head and the eyepiece tube to the base of the microscope. It supports the head of the microscope and is also used when carrying the microscope.

A

ARM

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11
Q

is the lowermost part of the microscope that supports the entire microscope structure. It provides stability for the microscope, Illuminators, light switches, and electrical wiring systems are fitted in the base.

A

base

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12
Q

is closest to the viewer’s eye. They are located at the top of the microscope. This part is used to look at the specimen.

A

The eyepiece (ocular Lens)

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13
Q

lenses come in different magnification powers from

A

5x to 30x

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14
Q

the most common ocular lenses are of?

A

10x or 15x magnification

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15
Q

It carries the eyepiece just above the objective lens.

A

eyepiece holder

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16
Q

In some microscopes, such as the binoculars, the ? is flexible and can be rotated for maximum visualization for variance in distance.

A

eyepiece tube

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17
Q

is a movable circular structure that houses all the objective lenses.

A

nose piece

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18
Q

nose piece is also called the?

A

REVOLVING TURRET

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19
Q

It is connected to the body tube and lies just above the stage. It can be rotated clockwise or counterclockwise to increase or decrease the magnification.

A

Nose piece

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20
Q

is the lens that is closest to the specimen. They are fitted on the nosepiece.

A

objective lens

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21
Q

A standard microscope has 3 to 4 objective lenses of different magnifying powers:

A

4X, 10X, 40X, and 100X.

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22
Q

are color-coded and are of different sizes. Size and color depend on the power of the lens.

A

Objective lenses

23
Q

The smallest lens is of the?

A

lowest power

24
Q

the longest lens will be of the

A

highest power

25
Q

In most optical microscopes, objective lenses with 100X or more magnification are of?

A

oil immersion type.

26
Q

provides the lowest magnification power of all objective lenses.

A

scanning objective lens ( red )

27
Q

4x is a common magnification for scanning objectives and, when combined with the magnification power of a 10x eyepiece lens, a 4x scanning objective lens gives a total magnification of?

A

40x

28
Q

The name “ ? “ of objective lens comes from the fact that they provide observers with about enough magnification for a good overview of the slide, essentially a “scan” of the slide

A

scanning

29
Q

has more magnification power than the scanning objective lens, and it is one of the most helpful lenses when it comes to observing and analyzing glass slide samples.

A

low power objective lens ( yellow )

30
Q

The total magnification of a low power objective lens combined with a 10x eyepiece lens is?

A

100x magnification

31
Q

high-powered objective lens (also called “ ? “ lens)

A

high dry

32
Q

is ideal for observing fine details within a specimen sample.

A

high-powered objective lens ( blue )

33
Q

The total magnification of a high-power objective lens combined with a 10x eyepiece is equal to?

A

400x magnification,

34
Q

provides the most powerful magnification

A

oil immersion objective lens

35
Q

What is oil immersion objective lens magnification when combined with a 10x eyepiece?

A

magnification total of 1000x

36
Q

Without adding a drop of ?, the oil immersion objective lens will not function correctly, the specimen will appear blurry, and you will not achieve an ideal magnification or resolution.

A

immersion oil

37
Q

used for focusing the image under low power magnification. It is a larger knob and is used to move the stage up or down very rapidly. The stage is raised or lowered rapidly

A

Coarse Adjustment Knob

38
Q

is used for fine adjustment. It is a smaller knob and is used to move the stage up or down very slowly. It is used to sharpen the image. It is mostly used while viewing under high power.

A

Fine Adjustment Knob

39
Q

This is the section in which the specimen is placed for viewing. They have stage clips that hold the specimen slides in place.

A

STAGE

40
Q

most common stage is the ?, which allows the control of the slides by moving the slides using the mechanical knobs on the stage instead of moving them manually

A

mechanical stage

41
Q

Holds the slide in place on the stage so it doesn’t move while you’re viewing the specimen.

A

STAGE CLIPS

42
Q

are the control knobs used to move the stage mechanically.

A

Stage Control Knobs

43
Q

There are two stage control knobs;

A

one for moving left and right and the other for moving forward and backward.

44
Q

These are lenses that are used to collect and focus light from the illuminator into the specimen. They are found under the stage next to the diaphragm of the microscope. They play a major role in ensuring clear, sharp images are produced with a high magnification of 400X and above.

A

CONDENSER

45
Q

More sophisticated microscopes come with an ?, that has a high magnification of about 1000x

A

Abbe condenser

46
Q

It’s also known as the iris.

A

DIAPHRAGM

47
Q

It is found under the stage of the microscope, and its primary role is to control the amount of light that reaches the specimen. It’s an adjustable apparatus, hence controlling the light intensity and the size of the beam of light that gets to the specimen.

A

DIAPHRAGM

48
Q

is the light source for a microscope, typically located in the base of the microscope.

A

Illuminator

49
Q

Make sure all backpacks and unnecessary materials are out of the aisles and off the tops of the desks.

A

TABLE PLACEMENT

50
Q

Install the microscope on a sturdy, level table. Equipment and instruments which generate vibrations should not be placed on or near this table. The height of the table should be convenient for the user. As an alternative or in addition, an adiustable stool should be made available to make

A

TABLE PLACEMENT

51
Q

Use only ? For cleaning lenses

A

lens paper or gauze and cleaning solution

52
Q

Lenses must be clean for resolution. Use only lens paper or gauze and cleaning solution. Never use your finger, handkerchief, paper towels or spit to clean the lenses. Do not remove any parts for cleaning; it only allows dust to enter the microscope

A

CLEANING

53
Q
  1. Turn off light & center mechanical stage.
  2. Position the nosepiece so that the lowest scanning (4X)
    objective is in place.
  3. Remove the slide from the stage, put in proper place.
  4. Clean the stage and lenses with gauze and lens cleaner, wipe off any oil.
  5. Wrap the cord around the arm. CAREFULLY carry with two hands and GENTLY place the microscope in the proper cabinet.
A

PUTTING AWAY