Microscope

Question 1

an optical instrument that is used to
observe tiny objects, often objects that
cannot be seen at all with the unaided
human eye (the “naked eye”).

Answer 1

MICROSCOPE

Question 2

Is the process of enlarging something only in appearance and not in physical size

Answer 2

Magnification

Question 3

Is the fineness, sharpness, and clarity of the produced image when viewed through an optical instrument. It is the ability of the instrument to produce a detailed image of the subject

Answer 3

Resolution

Question 4

– Used to observe morphology of microorganisms such as bacteria, protozoa, fungi, and algae in living (unstained) and nonliving (stained) state
– Cannot observe microbes less than 0.2 um in diameter or thickness, such as spirochetes and viruses

Answer 4

Brightfield Microscope

Question 5

– Unstained organisms are observed against a dark background
– Useful for examining thin spirochetes
– Slightly more difficult to operate than brightfield

Answer 5

Darkfield Microscope

Question 6

– Can be used to observe unstained living
microorganisms

Answer 6

Phase-Contrast Microscope

Question 7

– Fluorescent dye attached to organism
– Primarily an immunodiagnostic technique
(immunofluorescence)
– Used to detect microbes in cells, tissues, and clinical specimens

Answer 7

Fluorescence Microscope

Question 8

– Specimen is viewed on a screen
– Excellent resolution
– Allows examination of cellular and viral ultrastructure
– Specimen is nonliving

Answer 8

Transmission- Electron Microscope (TEM)

Question 9

– Specimen is viewed on a screen
– Gives the illusion of depth (three-dimensions)
– Useful for examining surface features of cells and viruses
– Specimen is nonliving
– Resolution is less than that of TEM

Answer 9

Scanning Electron Microscope

Question 10

defined as a microscope containing only
one magnifying lens.

Answer 10

SIMPLE MICROSCOPE

Question 11

a microscope that contains more than one
magnifying lens

Answer 11

COMPOUND MICROSCOPE

Question 12

The microscope objective lens should be
cleaned with? before and after
each use

Answer 12

lens paper

Question 13

Transporting the Microscope

Answer 13

• A microscope should be left in a permanent position on a study laboratory table in an area where it will not get jammed.
• If the microscope must be moved, it should be held securely with one hand supporting the base and the other hand holding the arm.
• The microscope should be placed
  gently on tabletops, to avoid jarring

Question 14

Storage of Microscope

Answer 14

• When the microscope is not being used, it
  should be left with the low power objective
  in position
• The stage should be centered so that it
  does not project from either side of the
  microscope
• The microscope should be stored in a plastic dust cover.