Microbiology techniques Flashcards
1
Q
What is Gram staining (4)
A
- Used to identify bacteria
- Two major groups
- Gram ve- = pink, thin-walled (vesicles )
- Gram ve+ = purple, thick-walled (channels)
2
Q
What is used to isolate colonies
A
the streak plate method
3
Q
what is a viable plate count
A
final plate in the series should have between 25 and 250 colonies
4
Q
How do you calculate the number of colony-forming units for pour plates
A
No. CFU/mL = dilution factor x average number of colonies
5
Q
How do you calculate the number of colony-forming units for spread plates
A
No. CFU/mL = 1/volume (ml) x dilution factor x average number of colonies
