enzyme kinetics

Question 1

Define the enzymatic parameter [E]o

Answer 1

Enzyme concentration at 0 seconds

Question 2

Define the enzymatic parameter v (2)

Answer 2

V = initial rate (Ms-1)

Question 3

Define the enzymatic parameter K M (2)

Answer 3

1. KM (M) is the substrate concentration that produces an initial rate equal to ½Vmax.
2. It is equivalent the dissociation constant for all enzyme-bound species.

Question 4

Define the enzymatic parameter V max

Answer 4

Vmax = Maximum rate (Ms-1)

Question 5

Define the enzymatic parameter kcat (2)

Answer 5

1. Kcat (s-1) = catalytic constant or turnover number
2. Kcat is equal to the maximum number of substrate molecules converted to product per active site per unit time. (capacity or efficiency of the enzyme)