Microbiology Ch.4 (Prokaryotic Cell) Functional Anatomy of Prokaryotic & Eukaryotic Cells Flashcards

1
Q

What are the 2 types of living cells

A

eukaryotes and prokaryotes

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2
Q

what are the different types of prokaryotes

A

Bacteria and Archea

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3
Q

What are the different types of eukaryotes

A

animal
plant
fungi(yeasts/molds)
protozoa
algae

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4
Q

describe viruses

A

noncellular elements that don’t fit into any organizational scheme of living cells

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5
Q

describe the word “prokaryotic”

A

comes from Greek word for pre-nucleus (not real nucleus)

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6
Q

describe the word “eukaryote”

A

comes from Greek word for true nucleus (hold genetic material)

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7
Q

describe prokaryote

A

one circular chromosome not in membrane
no histones
no organelles
peptidoglycan cell wall– if bacteria
pseudomurein cell walls — if archae
binary fission

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8
Q

describe eukaryote

A

paired chromosomes in nuclear membrane
histones
organelles
polysaccharide cell walls
mitotic spindle

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9
Q

describe the shape of prokaryotic cells

A

average size of 0.2-1.0um x 2-8um
most bacteria are monomorphic
few are pleomorphic

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10
Q

basic shapes of prokaryotic cells

A

bacillus(rod shaped)
coccus (spherical)
spiral —spirillum, vibrio, spirochete

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11
Q

what are the different arrangements of prokaryotic cells

A

pairs, clusters, and chains

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12
Q

describe “pairs” arrangement and give example

A

Pairs—Diplococci
ex. Streptococcus pneumoniae & Neisseria gonorrhoeae

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13
Q

describe clusters arrangement and give example

A

clusters—-staphylococci
ex. S. aureus

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14
Q

describe arrangement of “chains” and give an example

A

chains— streptococci
ex. streptococcus pyogenes

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15
Q

describe tetrad

A

cocci are arranged in packets of 4 cells divided in 2 planes
pediococcus (salt tolerance) and tetragenococcus

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16
Q

describe Sarcinae

A

cocci are arranged in cuboidal manner and divide in 3 planes and remain in groups cube like group 8
ex. Sarcinae ureae

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17
Q

describe Bacilli

A

(plural) — single rod: bacillus cereus

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18
Q

describe diplobacilli

A

klebsiella rhinoscleromatis—UTI
2 rods

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19
Q

describe streptobacilli

A

bacilli are arranged in chains as the cells divide in one plane
ex. streptobacillus moniliformis— rat bite fever

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20
Q

describe coccobacillus

A

short and stumpy that they appear ovoid
they look like coccus and bacillus
ex. Haemophilus influenzae and Gardnerella vaginalis

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21
Q

describe palisade

A

bacilli bend at the points of division following the cell divisions resulting in a palisade arrangement

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22
Q

what does palisade resemble

A

it resembles a picket fence (wall) and angular patters that look like Chinese letters
ex. Corynebacterium diphtheriae

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23
Q

describe vibrio

A

comma-shaped bacteria with less than one complete tum or trust in the cell
ex. Vibrio cholerae

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24
Q

describe spirilla

A

rigid spiral structure
spirillum with many turns can superficially resemble spirochetes
they don’t have outer sheath and endoflagella but have typical bacterial flagella
ex. Helicobacter pylori

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25
describe spirochetes
helical shape and flexible bodies move by means of axial filaments ex. Treponema pallidum ex. syphilis
26
axial filaments
also called endoflagella in spirochetes anchored at one end of a cell beneath an outer sheath and wrap in spiral fashion around the cell rotation causes cell to movie in a corkscrew manner
27
describe unusually shaped Bacteria: Stella
flat six-pronged star-shaped bacteria found in various environments ex. freshwater, soil. sewage
28
describe unusually shaped bacteria Haloarcula spp. (H. vallismortis; H. marismortui
found in neutral saline environments such as salt lakes, marine salt terms and saline soils rectangular bacteria
29
Pleomorphic Bacteria
don't have characteristic shape unlike others; they can change their shape in pure cultures it can be observed to have different shapes ex. M. genitalium ---- sexually transmitted and can cause inflammation of the urinary and genital tracts in men and women
30
what does the structure of prokaryotic cells contain
pilus infusion capsule cell wall plasma membrane fimbriae ribosome cytoplasm nucleoid containing DNA plasmid flagella
31
glycocalyx
outside cell wall usually sticky slime layer--- unorganized and loose
32
what is the capsule description of glycocalyx
neatly organized and tightly attached extracellular polysaccharide allows cell to attach ex. Streptococcus pneumonia; Bacillus anthracis
33
flagella
outside cell made of chains of flagellin attached to a protein hook anchored to the wall and membrane by the basal body
34
what are the Parts & Attachments of (flagella) gram positive
gram-positive flagellum filament cell wall hook basal body peptidoglycan plasma membrane cytoplasm
35
What are the parts and attachments of (flagellum) gram negative
cell wall, basal body, flagellum, plasma membrane, cytoplasm, outer membrane, and peptidoglycan
36
What are the arrangements of bacterial flagella
paritrichous, monotrichous and polar; lophotrichious and polar; amphitrichous and polar
37
motile cells
rotate flagella to run or tumble move toward and away from stimuli (taxis) and stimulus ex. chemicals (chemotaxis); light (phototaxis)
38
what are flagella proteins
hantigens ex. E. coli 0157:H7 (food poisoning) -- antigens on the body of the bacteria are called somatic antigens or just O antigens
39
fimbriae
filamentous appendages that are shorter straighter and more numerous than flagella found mostly in gram negative bacteria used for attachment (mucus membrane) not motility consist of protein pilin
40
Pili
hair-like projections of the cell protein (pilin) longer than fimbriae only 1 or 2 per cell facilitate transfer of DNA from once cell to another ex. sexual conjugation (sex pill) adhesion to host epithelium it can be antiphagocytic movement--- gliding motility; twitching motility
41
Cell Wall
semirigid structure prevent osmatic lysis made of peptidoglycan (in bacteria) polymer of disaccharide ex. N- acetylglucosamine amine (NAG) N-acetylmuramic acid (NAM) muramic acid d-amino acids diaminopimelic acid--- not synthesized by mammals
42
peptidoglycan in gram-positive bacteria
linked by polypeptides
43
what is the process taking place in the gram positive cell wall
teichoic acids lipoteichoic acids link to plasma membrane wall teichoic acid links to peptidoglycan may regulate movement of cations polysaccharides provide antigenic variation
44
what is the process taking place in the gram negative cell wall
thin peptidoglycan outer membrane periplasmic space
45
gram negative outer membrane
lipopolysaccharides; lipoproteins, phospholipids forms the periplasm between the outer membrane and the plasma membrane protected from phagocytes, complement, and antibiotics O polysaccharide antigen ex. E. coli 0157 H7 lipid A is an endotoxin porins (proteins) form channels through membrane
46
gram positive cell wall
2-ring basal body disrupted by lysozyme penicillin sensitive
47
gram negative cell wall
4-ring basal body endotoxin tetracycline sensitive
48
atypical cell walls
acid-fast cell walls like gram positive waxy lipid (mycolic acid) bound to peptidoglycan Mycobacterium and Nocardia
49
mycoplasmas
lack cell walls steroids in in plasma membrane
50
archaebacteria
constitutes a domain of single-celled organisms lack cell nuclei and are therefore prokaryotes may be responsible for some diseases with no known causes such as Chron's disease, arthritis, lupus, and gingivitis don't have peptidoglycan in their walls walls of pseudomurein lack NAM and D-amino acids
51
Damage to the cell
lysozyme enzyme found in tears, mucus, saliva digests disaccharide in peptidoglycan of many Gram positive bacteria penicillin inhibits peptide bridges in peptidoglycan protoplast--- bacterium has lost its cell wall and surrounded only by plasma membrane ---derived from gram-positive spheroplast cell wall only partially removed and are gram negative both are sensitive to rupture by osmotic layers
52
plasma membrane
phospholipid bilayer peripheral proteins integral proteins transmembrane proteins damage to membrane by alcohols, quaternary ammonium (detergents) and polymyxin antibiotics causes leakage of cell contents
53
fluid mosaic model
membrane is as vicious as olive oil proteins move to function phospholipids rotate and move laterally oxidative phosphorylation occurs at cell membrane since there are no mitochondria houses many biosynthetic enzymes for metabolic reactions such as nutrient breakdown, energy (ATP) and photosynthesis
54
cytoplasmic membrane
selective permeability extracellular fluid , inside of cell, membrane protein, bilayer membrane
55
plasma membrane
selective permeability allows passage of some molecules chromoplasts or thylakoids contain pigments for bacterial biosynthesis are found in a wide range of animals including amphibians, fish, and reptiles
56
movement of materials across membrane
simple diffusion, facilitated diffusions, active transport,--- through lipid layer and aquaporins (water channels) osmosis and osmotic pressure
57
simple diffusion
movement of solute from an area of high concentration to an area of low concentration
58
facilitated diffusion
solute combines with a transporter protein (permease) in the membrane
59
active transport
materials move from areas of low to high concentration by transporter proteins and the cell must expand requires a transporter protein and ATP
60
osmosis
the movement of water across a selectively permeable membrane from an area of high water to an area of lower water concentration
61
osmotic pressure
pressure needed to stop the movement of water across the membrane
62
principles of osmosis
isotonic solution hyprotic solution hypertonic solution
63
isotonic solution
no net movement of water equal solute concentration on both sides
64
hyprotic solution
water moves into cell wall if cell wall is strong it contains the swelling if cell wall is weak or damaged the cell bursts (osmotic lysis)
65
hypertonic solution
water moves out cell causing its cytoplasm to shrink (plasmolysis)
66
cytoplasm
matric inside the plasma membrane 80% water contains inclusions, ribosomes, vacuoles, and nuclear are primary sites for biosynthesis
67
nuclear are/nucleoid
bacterial chromosome-- bacterial genome all information needed to make and reproduce a bacterial cell of a species all essential information of that species circular double stranded DNA bacterial cell may have more than one chromosome but these are duplicates and only one is needed
68
prokaryotic ribosome
protein synthesis 70S+30S subunits 30S 50S 50S 30S small subunit + large subunit --> complete (70S) ribosomes
69
selective toxicity
some antibiotics are aimed at the 70S ribosomes of bacterial cells streptomycin, neomycin, erythromycin and tetracycline work by inhibiting protein synthesis by disrupting the 70S ribosome
70
inclusions
metachromatic granules (volutin)---> phosphate reserves polysaccharide granules (glycogen, starch)---> energy reserves glycogen; starch lipid inclusions & sulfur granules---> energy reserves carboxysomes--> ribulose 1,5-diphosphate carboxylase for---> gas vacuoles magnetic (force into alignment --> magnetosomes
71
Plasmids
small circular, extra chromosomal of DNA 5-100 genes code for auxiliary metabolic functions ---antibiotic resistance --- penicillinase production of toxins --- E.coli 0157H these are transferred very easily through conjugation replicate autonomously
72
endospores
resting cells dormant cells-- not metabolically active produced when starved resistant to desiccation, heat, chemicals, lack of water contain calcium dipicolinate vegetative bacterial cells are killed at temps above 70C (160F) can survive boiling water for several hours even up to 20hrs sporulation-- endospore formation germination--- return to vegetative state
73
sporulation
endospore formation
74
germination
return to vegetative state
75
how do endospores remain under periods of environmental stress
remain viable for weeks, months, years
76
thermoactinomyces vulgaris
spores found in Minnesota that were 7,500 yrs old and still germinated
77
endospores are only found in what kind of bacteria
gram positive bacteria bacillus cereus bacillus anthracis clostridium clostridium tetani clostridium botulinum clostridium perfringens
78
formation of endospores by sporulation
1)spore septum begins to isolate newly replicated dna and a small portion of cytoplasm 2)plasma membrane starts to surround dna , cytoplasm, and membrane isolated in step 1 3)spore surrounds isolated portion forming forespore 4)peptidoglycan layer forms between membranes 5)spore coats forms 6)endospore is freed from cell
79
spore
Schaeffer Fulton stain modified gram positive bacteria cell unusual cell envelope cell membrane outer membrane