Microbiology Flashcards

1
Q

Patient presents with vomiting, epigastric pain and diarrhoea, 6 hours after eating a meat and cheese pie.

What is the cause?

How do you treat them?

A

Staphylococcus aureus (exotoxin)

Supportive care: fluids, electrolytes

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2
Q

Patient presents with vomiting, epigastric pain and diarrhoea, 6 hours after eating reheated rice.

What is the cause?

A

Bacillus cereus (exotoxin)

Supportive care: fluids, electrolytes

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3
Q

Pt presents with vomiting, headache and watery diarrhoea.

A stool PCR detects norovirus. Where did they likely pick this up?

A

Cruise ship. Nursing home.

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4
Q

Patient has just returned from their travels. They present with watery diarrhoea.

What is the likely cause? What is the treatment?

A

Enterotoxin E Coli

Ciprofloxacin

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5
Q

Patient presents with severe watery, “rice-water” stool.

A stool culture detects a pathogen; what is the likely cause? What is the treatment?

A

Vibrio CHOLERAE

Supportive care - IV fluids.

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6
Q

Patient presents with weeks of loose diarrhoea, cramps, frequent belching and flatulence, and a fatty foul stool.

Their ImmunoCard STAT shows an antigen.

What is the likely cause? What will their stool microscopy show?

What is the treatment?

A

Giardia Lamblia

Stool microscopy: ova + parasite cysts

Metronidazole

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7
Q

Patient presents with watery diarrhoea and is febrile. They recently ate cheese with raw cold vegetables.

What is the likely cause?

A

Listeria monocytogenes

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8
Q

Causes of non-inflammatory diarrhoea (ie watery, non-bloody)

A

SBEVNGL

Staph aureus 
Bacillus cereus 
Enterotoxin E coli
Vibrio cholerae 
Norovirus 
Giardia Lamblia 

Listeria monocytogenes (febrile)

Some
Bitches
Eat 
Vulgar 
Non-inflammatory
Goats, 
Leisurely
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9
Q

Patient presents with bloody diarrhoea. She recently had some rare beef with fresh veg from a farm,

What will her stool culture likely show?

What is the treatment?

A

Shigella-toxin E Coli

None.

Do NOT give Abx to children - can cause Haemolytic Uraemic Syndrome (HUS)

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10
Q

Patient comes in with diarrhoea containing blood and pus.

Their stool culture is positive. Likely cause? Treatment?

A

Shigella

Ciprofloxacin

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11
Q

Patient comes in with bloody diarrhoea and fever. They recently ate some undercooked eggs with raw vegetables and pink chicken.

Likely cause? Treatment?

A

Salmonella

Ciprofloxacin

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12
Q

Patient presents with fever and diarrhoea, after eating unpasteurised cheese.

What is the likely cause? Treatment?
What neurological condition are they at a higher risk of?

A

Campylobacter jejuni

Azithromycin/ Ciprofloxacin

Guillain Barre Syndrome

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13
Q

Patient develops bloody diarrhoea and fever whilst in hospital.

What is the likely cause? What may be seen on a colonoscopy?

Treatment?

A

C. difficile

Colonoscopy: pseudomembranous colitis

Metronidazole

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14
Q

What are the causes of inflammatory diarrhoea?

A

SSSCC

STEC 
Shigella 
Salmonella 
Campylobacter jejuni
C difficile
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15
Q

Causes of appendicitis

A

Normal colonic flora:

Escherichia coli
Peptostreptococcus
Bacteroides fragilis
Pseudomonas

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16
Q

Causes of Diverticulitis

A

Bowel flora

B. fragilis
E. coli

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17
Q

Malaria pathogens + transmission

A
P falciparum - 80% 
P Vicax 
P Ovale (mild)
P Malariae (persists yrs)
P Knowlesi 

Sporozoites via Anopheles mosquito > hepatocytes > schizonts > rupture > merozoites

18
Q

Malaria presentations

A

Fever/sweats
Haemolytic anaemia
Hepatosplenomegaly

19
Q

Malaria investigations

A

Thick + Thin Blood Smear with FIELD’s/ GIEMSA stain (light microscopy)

1L: FBC - haemolytic anaemia, thrombocytopenia, abnormal LFTs

20
Q

Malaria treatments

A

QUINOLINES

  • chloroquine
  • Quinine

ANTIFOLATES

  • pyrimethamine
  • Sulfonamides

RIBSOSOME INHIBITORS

  • Tetracycline
  • Doxycycline
  • Clindamycin

Supportive

21
Q

Pt presents with quickly spreading erythema on the legs, which is tender and warm to touch. There is a wound at the site.

What is the likely diagnosis? Treatment?

A

Cellulitis: B-haemolytic streptococci - S. pyogenes, S. agalactiae. Staph aureus.

Treatment:

  • elevate + mobilize limb
  • washout wound
  • Empiric IV Flucloxacillin (or Clindamycin)

If MRSA cellulitis: VANCOMYCIN

22
Q

Pt develops warm, tender erythema around surgical site following operation. Their skin is blue-gray (hypoperfused) and there’s a loss of sensation. Crepitus is found on exam, as well as Fournier’s gangrene.

Cause? Treatment

A

Necrotizing Fasciitis

Tx:

  • Emergency surgical exploration
  • Empirical piperacillin-tazobactam + clindamycin
  • S. pyogenes - penicillin + clindamycin
23
Q

Gram negative bacteria are grown on which type of agar?

A

MacConkey agar

24
Q

Pink colonies grow on a MacConkey Agar plate.

What clinically relevant bacteria could these be?

A
  • E coli
  • Klebsiella

= Lactose-fermenting Gram Negative Bacillus

= Enterobacteriae

25
Q

Name some gram negative aerobic COLIFORM species

A
  • Escherichia
  • Klebsiella
  • Salmonella
  • Shigella

EKSS

26
Q

Name some gram negative aerobic VIBRIO species

A
  • Vibrio cholerae
  • Campylobacter
  • Helicobacter
  • Haemophilus

VCHH

27
Q

Gram positive cocci are grown on which agar?

A

Blood Agar

28
Q

Cream yellow colonies grow on a blood agar plate. What’s the likely species? What other test can be performed to detect this bacteria?

A

Staphylococcus aureus

Coagulase/ DNAase test will be positive

29
Q

Gram positive chains =

A

Streptococcus

30
Q

Gram positive cocci =

A

Staphylococcus

31
Q

An optochin test is performed. The area around is clear.

A

Streptococcus pneumoniae

= Gram positive alpha-haemolytic streptococcus

32
Q

An optochin test is performed. It has absolutely no effect x

A

Viridians streptococcus

33
Q

Lancefield grouping is used to categorise…

A

Gram positive beta-haemolytic streptococci

34
Q

Streptococcus pyogenes is which lancefield group?

A

Group A Gram positive beta-haemolytic

35
Q

Streptococcus agalactiae is which Lancefield group?

A

Group B gram positive beta-haemolytic

36
Q

Enterococcus faecalis is which Lancefield group?

A

Group D gram positive beta-haemolytic

37
Q

What culture is used to grow Mycobacterium tuberculosis?

A

Lowenstein-Jensen

38
Q

What culture is used to grown anaerobes? Give an example of anaerobe

A

Blood agar

Fusobacteria

39
Q

What culture is used to grow aerobes? give an example of an aerobe

A

Chocolate agar

Strep pneumoniae

40
Q

What cutlture is used to grow gram-negative bacilli?

A

MacConkey agar

41
Q

1, 3 beta-D-glucan assay

Examples of organisms

A

= Chitin :. detects fungi

Aspergillus fumigatus
Candida albicans
Pneumocystitis jirovecii

42
Q

Cholera pathogenesis

A

Toxin. Deregulates ion transport in epithelial cells