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Pretest 2 > MICROBIOLOGY > Flashcards

MICROBIOLOGY Flashcards

1
Q

Grow in ambient air, which contains 21% O2 and small amount (0.03%) of CO2

A

Aerobes

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2
Q

Cannot grow in the presence of O2 and the atmosphere in anaerobe jars

A

Anaerobes

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3
Q

Grow under reduced O2 and increased CO2

A

Microaerophilic

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4
Q

Requires increased concentration of CO2 and approximately 15% O2

A

Capnophiles

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5
Q

Allow room (unsterilized) air to pass into the cabinet and around the material within, sterilizing only the air to be exhausted

A

Class I BSC

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6
Q

Self contained and 70% of the air is recirculated

A

Class IIA BSC

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7
Q

Air is discharged outside the building. It is selected if radioisotopes, toxic chemicals or carcinogens will be used

A

Class IIB BSC

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8
Q

Air coming into and going out of the cabinet is filter sterilized and the infectious material within is handled with rubber gloves that are attached and sealed to the cabinet

A

Class III

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9
Q

Classification of organisms according to hazard/ biosafety levels where no known potential for infecting the healthy people and are well defined and characterized

A

BSL-1

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10
Q

Classification of organisms according to hazard/ biosafety levels where most commonly being sought in clinical specimens and used in diagnostic, teaching and other laboratories

A

BSL-2

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11
Q

Classification of organisms according to hazard/ biosafety levels where recommended for the handling of material suspected of harboring organisms unlikely to be encountered ina routine clinical laboratory and for such organisms as M. Tuberculosis, C. Burnetii, the mold stages of systemis fungi and formsome other organisms when grown in quantities greater than that found in patient specimen

A

BSL-3

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12
Q

Classification of organisms according to hazard/ biosafety levels where exotic agents are considered high risk and cause life threatening disease

A

BSL-4

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13
Q

Chain of infection

A

Infectious organism / pathogen
Reservoir portal of exit
Mode of transmission
Portal of entry
Susceptible host

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14
Q

Safest method to ensure that no infective materials remain in samples or containers when disposed

A

Incineration

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15
Q

Used to sterilize biohazardous trash and heat-stable objects

A

Autoclave

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16
Q

Used to sterilize items such as glassware, oil, petrolatum or powders

A

Dry heat oven

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17
Q

Method of choice for antibiotic solutions, toxic chemicals, radioisotopes, vaccines and carbohydrates which are all heat sensitive

A

Filtration

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18
Q

Used for sterilizing disposables such as plastic syringes, catheters, or gloves before use

A

Ionizing radiotion

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19
Q

Most common chemical sterilant

A

Ethylene dioxide

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20
Q

Use of glutaraldehydeor peracetic acid

A

Cold sterilization

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21
Q

Mechanical action of rubbing the hands together and soaping undeneath fingernails

A

Most important part of handwashing

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22
Q

Round, smooth, convex, some strains are beta hemolytic; most colonies appear black or very dark purple; cultures smell of ammonium cyanide

A

Chromobacterium violaceum

23
Q

Cause granulomatous disease in animals and have been associated with soft tissue infection in humans following animal bites

A

Actinobacillus spp.

24
Q

Recovered from mixed populations of organisms and identified using the ethanol shock sore technique

A

Clostridium spp.

25
Q

Serpentine pattern of growth

A

M. Tuberculosis

26
Q

Diagnosis of leprosy is based on distinct clinical manifestation, such as hypopigmented skin lesions and peripheral nerve involvement in conjunction with a smear that tests positive for afb

A

M. Leprae

27
Q

After ingestion of milk from infected cows, organism my penetrate the gastrointestinal mucosa or invade the lymphatic tissue of the oropharynx

A

M. Bovis

28
Q

Causes an inflammatory bowel disease (known as Johne’s disease) iqn attle, sheep and goats. It also has been isolated fro, the bowel mucosa of patients with Crohn’s disease a chronic inflammatory bowel disease of humans

A

M. avium subp. Paratuberculosis

29
Q

Mot common cause of aseptic meningitis, an inflammation of the brain parenchyma, and have been isolated from more than 40# of patients with this disease

A

Enterovirus

30
Q

Defective measles virus infection of CNS

A

Subacute sclerosing panencephalitis

31
Q

Detection of HSV / VZV

A

TZANCK smear

32
Q

Maginfication for detection of microfilaria

A

Low magnification

33
Q

Magnification for plasmodia and trypanosoma

A

Oil immersions

34
Q

Causes Loeffler’s syndrome: transient pulmonary infiltration: day today clearing in 3 - 14 days; associated with marked peripheral eosinophilia

A

Ascaris Lumbricoides

35
Q

Worm has a characteristic, thick cuticle and a large uterus that fills the body cavity and contains rhabditoid larvae

A

Dracunculus medinensis

36
Q

Adult female worm has a distinctive morphologic appearance with spiral, winding, BARBER POLE appearing uterus

A

Parastongylus cantonensis

37
Q

May be associated with vertical transmission and congenital infections

A

Ancylostoma duodenale

38
Q

Most common identified species of filarial worms that infect humans

A

Wucheriria bancrofti

39
Q

Resembles egg of S. Haematobium, but acid fast positive

A

Schistosoma intercalatum

40
Q

To optimize recovery of S. Haematobium in urine what time it should be collected

A

Between noon and 2pm

41
Q

Tapeworm specialized structure for attachment

A

Scolex

42
Q

Presence of RBCs is the onlg characteristic that allows differentiation between pathogenic E. Histolytica and non pathogenic E. Dispar

A

Entamoeba dispar

43
Q

Basket nucleus

A

Iodamoeba butschlii

44
Q

Gay bowel syndrome

A

Giardia lamblia

45
Q

Organism found most commonly in the crypts in the duodenum

A

Giardia lamblia

46
Q

Still considered the gold standard for diagnosis of malaria

A

Examination of thick and thin films

47
Q

Oocysts recovered from a sheather’s sugar floatation

A

Cryptosporidium

48
Q

Recovery of s. Stercoralis from stool

A

Baermann funnel technique

49
Q

Ecofriendly stool fixative

A

Polyvinyl alcohol

50
Q

Test for cyclospora cayetanensis

A

Autofluorescnece, modified acid fast

51
Q

Weak decolorizee, coccidia

A

Modified acid fast stain

52
Q

10x dye content, microsporidia

A

Modified trichrome stain

53
Q

Donning

A

Gown, mask/respirator, goggle/faceshield, gloves

54
Q

Doffing

A

Gloves, googles,/face shield, gown, mask/ respirator

Pretest 2 (7 decks)

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