Histopathology And MT Laws Flashcards
Accidental skin contact with hazardous chemicals; wash hands with water for
15 to 30 mins
Process done by the automatic tissue processor
Fixation, dehydration, clearing and infiltration
Stations 1-2 :
Stations 3-6 :
Stations 7-8 :
Stations 9-10 :
Stations 11-12 :
10% formalin
Ascending grades of ethyl alcohol 70- 95%
2 changes of acetone
2 changes of chooroform or xylol
2 changes of liquid paraffin
Renoval of nicks
Honing
Removal of burrs
Stropping
Nuclear and histochemical fixative
Newcomer’s fixative
Most common metallic fixative
Mercurial fixative
Fixative for tissue photography
Mercurial fixative
Fixative for small tissue fragements
Oicric acid; yellow color to locate tissue easier
Excellent fixative for preservkng soft amd delicate structures
Bouin’s solution
Picric acid fixative
Bouin’s, Brasil’s
Fixative for electrom microscopy
Glutaraldehyde, osmium tetroxide
Air-filled lungs may float on fixative what should be done
Organ may be covered with several gauze to maintain it under surface
Decalcifying agent that contains HCl
Von Ebner’s (HCl, NaCl, d. Water)
Embeddiing medium for electron microscopy
Plastic medium
Double embedding
Infiltrated with celloidin,
Embedded with paraffin
Fastest embeding method
Vacuum embedding
Quenching temperature in freeze-drying
-160 to -180 degree celsius
Dessication/ sublimation temp for freeze drying
-30 to -40 degree celsius
Warer must be removed by heating the waxmat what temp
100 to 105 degree celsius
Paraffin wax oven temp
55 to 60 or 2 to 5 degree celsius mp wax
Floatation water bath temp
45 - 50 degree celsius or 6 to 10 degree celsius below mp
Simplest microtome, Paldwell trefall
Rocking microtome
Most common microtome, Minot
Rotaty microtome
Most dangerous type of microtome, Adams
Sliding microtome
Releases CO2, Queckett
Freezing microtome
Electrically driven refrigerated chamber, where microtome, knife, specimen and atmosphere are kept at rhe same temperature
Cryostat
Temp of cryostat
-20
Bevel angle of microtome
27 to 32 degree
Clerance angle of microtome
0 to 15 degree angle
5 to 10 degree angle to prevent uneven sections or alternate thick and thin section
Regressive staining, it involves a differentiation step (acid alcohol)
Manual H and E staining
Eosin, phloxine, picric acid, orange G, Rose Bengal, light green, lissamine green are examples of
Cytoplasmic stains
Neutral red, safranin, carmine, hematoxyljn, methylene blue, toluidine blue, celestine blue are what kind of stain
Nuclear stain
Specific reaction for DNA
Feulgen technique
What is the DNA and RNA color in methyl green
DNA- green or blue green
RNA- rose red
Color of acid mucopolysaccharide, fungi, background in fluorescent acridine irange technique
Acid mucopolysaccharide- black
Fungi- greenish red fluorescence
Background- reddish orange fluorescence
Histones and protamines in alkaline fast green
Green
Stain for the basement membrane
PAS, azocarmine
Demonstration of muscle striations
Mallory’S PTAH
Stain for helicobacter pylori
Toluidene blue, cresyl violet acetate
Oldest stain, glycogen mahogany brown
Iodine
Reticulin fibers are stained black
Gomori’s stain
Best location for specimen of vaginal smears
Upper lateral third of the vaginal wall
Respiratory tract specimens are
Sputum, bromchoalveolar lavage/ bronchial washing,
Bronchial brushing
Gastrointestinal specimens are
Gastric lavage, gastric brush and FNA
If delayed, CSF specimen for cytology should be
Refrigerated
Reversible change from one adult dell type to another
Metaplasia
Most of the nuclei of females exhibit conglomerqtion ir chromatin, demomstrated in the smears from buccal or vaginal mucosa
Barr bodies, XX chromosomes
Autopsy where organs are removed one by one
Virchow
Autopsy which involves insitu dissection
Rokitansky
En bloc autopsy
Ghon
En masse autopsy
Letulle
Anti rabies act of 2007
RA 9482