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Pretest 2 > Histopathology And MT Laws > Flashcards

Histopathology And MT Laws Flashcards

1
Q

Accidental skin contact with hazardous chemicals; wash hands with water for

A

15 to 30 mins

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2
Q

Process done by the automatic tissue processor

A

Fixation, dehydration, clearing and infiltration

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3
Q

Stations 1-2 :
Stations 3-6 :
Stations 7-8 :
Stations 9-10 :
Stations 11-12 :

A

10% formalin
Ascending grades of ethyl alcohol 70- 95%
2 changes of acetone
2 changes of chooroform or xylol
2 changes of liquid paraffin

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4
Q

Renoval of nicks

A

Honing

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5
Q

Removal of burrs

A

Stropping

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6
Q

Nuclear and histochemical fixative

A

Newcomer’s fixative

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7
Q

Most common metallic fixative

A

Mercurial fixative

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8
Q

Fixative for tissue photography

A

Mercurial fixative

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9
Q

Fixative for small tissue fragements

A

Oicric acid; yellow color to locate tissue easier

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10
Q

Excellent fixative for preservkng soft amd delicate structures

A

Bouin’s solution

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11
Q

Picric acid fixative

A

Bouin’s, Brasil’s

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12
Q

Fixative for electrom microscopy

A

Glutaraldehyde, osmium tetroxide

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13
Q

Air-filled lungs may float on fixative what should be done

A

Organ may be covered with several gauze to maintain it under surface

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14
Q

Decalcifying agent that contains HCl

A

Von Ebner’s (HCl, NaCl, d. Water)

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15
Q

Embeddiing medium for electron microscopy

A

Plastic medium

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16
Q

Double embedding

A

Infiltrated with celloidin,
Embedded with paraffin

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17
Q

Fastest embeding method

A

Vacuum embedding

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18
Q

Quenching temperature in freeze-drying

A

-160 to -180 degree celsius

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19
Q

Dessication/ sublimation temp for freeze drying

A

-30 to -40 degree celsius

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20
Q

Warer must be removed by heating the waxmat what temp

A

100 to 105 degree celsius

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21
Q

Paraffin wax oven temp

A

55 to 60 or 2 to 5 degree celsius mp wax

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22
Q

Floatation water bath temp

A

45 - 50 degree celsius or 6 to 10 degree celsius below mp

23
Q

Simplest microtome, Paldwell trefall

A

Rocking microtome

24
Q

Most common microtome, Minot

A

Rotaty microtome

25
Q

Most dangerous type of microtome, Adams

A

Sliding microtome

26
Q

Releases CO2, Queckett

A

Freezing microtome

27
Q

Electrically driven refrigerated chamber, where microtome, knife, specimen and atmosphere are kept at rhe same temperature

A

Cryostat

28
Q

Temp of cryostat

A

-20

29
Q

Bevel angle of microtome

A

27 to 32 degree

30
Q

Clerance angle of microtome

A

0 to 15 degree angle
5 to 10 degree angle to prevent uneven sections or alternate thick and thin section

31
Q

Regressive staining, it involves a differentiation step (acid alcohol)

A

Manual H and E staining

32
Q

Eosin, phloxine, picric acid, orange G, Rose Bengal, light green, lissamine green are examples of

A

Cytoplasmic stains

33
Q

Neutral red, safranin, carmine, hematoxyljn, methylene blue, toluidine blue, celestine blue are what kind of stain

A

Nuclear stain

34
Q

Specific reaction for DNA

A

Feulgen technique

35
Q

What is the DNA and RNA color in methyl green

A

DNA- green or blue green
RNA- rose red

36
Q

Color of acid mucopolysaccharide, fungi, background in fluorescent acridine irange technique

A

Acid mucopolysaccharide- black
Fungi- greenish red fluorescence
Background- reddish orange fluorescence

37
Q

Histones and protamines in alkaline fast green

A

Green

38
Q

Stain for the basement membrane

A

PAS, azocarmine

39
Q

Demonstration of muscle striations

A

Mallory’S PTAH

40
Q

Stain for helicobacter pylori

A

Toluidene blue, cresyl violet acetate

41
Q

Oldest stain, glycogen mahogany brown

A

Iodine

42
Q

Reticulin fibers are stained black

A

Gomori’s stain

43
Q

Best location for specimen of vaginal smears

A

Upper lateral third of the vaginal wall

44
Q

Respiratory tract specimens are

A

Sputum, bromchoalveolar lavage/ bronchial washing,
Bronchial brushing

45
Q

Gastrointestinal specimens are

A

Gastric lavage, gastric brush and FNA

46
Q

If delayed, CSF specimen for cytology should be

A

Refrigerated

47
Q

Reversible change from one adult dell type to another

A

Metaplasia

48
Q

Most of the nuclei of females exhibit conglomerqtion ir chromatin, demomstrated in the smears from buccal or vaginal mucosa

A

Barr bodies, XX chromosomes

49
Q

Autopsy where organs are removed one by one

A

Virchow

50
Q

Autopsy which involves insitu dissection

A

Rokitansky

51
Q

En bloc autopsy

A

Ghon

52
Q

En masse autopsy

A

Letulle

53
Q

Anti rabies act of 2007

A

RA 9482

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