Clinical Microscopy Flashcards
Seen under LPF
Casts, Squamous epithelial cells, Mucus, Abnormal crystals
Seen under HPF
RBCs, WBCs, Transitional epithelial cells, RTE cells, Oval fat bodies, Bacteria, yeasts, Trichomonas, Normal crystals
Degree of hazard for NO or minimal hazard
0
Degree of hazard for slight hazard
1
Degree of hazard for moderate hazard
2
Degree of hazard for serious hazard
3
Degree of hazard for extreme hazard
4
Used to disinfect hands that are not visibly soiled
Alcohol based handrub
Hands visibly soiled
Washed with soap and water
Step by step documentation of handling and testing of legal specimens. It begins with patient identification and continues until testing is completed and results reported
Chain of custody (Chain of Evidence)
Required volume for drug testing
30-45mL
Capacity of container for drug testing
60mL
Normal temp of urine for drug testing
32.5 to 37.7 degree celsius
Required volume for routine urinalysis
10-15 mL
Average: 12mL
Capacity of urine container for routine urinalysis
50 mL
Which part is glucose reabsorbed
Proximal convoluted tubule
Location of the passive reabsorption of water takes place in all parts of the nephron except
Ascending loop of henly
Responsible for yellow urine
Carotene
Responsible for the red brown urine in children
Fuchsin (candy, food)
Print blurred through urine
Cloudy
Calibration of refractometer with 5% NaCl
1.022 +- 0.001
Calibration of refractometer with 9% sucrose
1.034 +- 0.001
Disorder with a cabbage odor urine
Methionine malabsorption
Concentration of sulfosalicylic acid for protein precipitation test
3% SSA
Significant value of AER: albumin excretion rate
20-200 ug/min
Principle of micral test
Enzyme Immunoassay
11th pad in the reagent strip
Vit C, ascorbic acid
Differentiates hemoglobin from myoglobin
Blondheim’s test
Ammonum sulfate ppt. Hemoglobin
Interference contrast microscope
Nomarski microscope
RBCs in hypotonic
Ghost cells
WBCs in hypotonic urine
Glitter cells
Lipid - containing RTE cells
Oval far bodies, lipiduria, nephrotic syndrom
RTE cells with nonlipid containing vacuoles
Bubble cells, acute tubular necrosis
Where are casts formed
In DCT and CT
Casts increased in congestive heart failure
Hyaline casts
Lemon shaped crystals
uric acid
Crystals found in ethylene glycol poisoning
Monohydrate calcium oxalate
Oval, dumbbell
Characteristic of ampicillin crystals
Colorless needles
Yellow brown, occasionally colorless sheaths of wheat, rosettes, meedles.
Confirmed by the Diazo reaction
Sulfonamide crystals
Flat rectangular plates or prisms
Calcium phosphate, apatite
Calcium phosphate also known as
Apatite
Dihydrate calcium oxalate
Envelope, pyramidal
Weddelite
Monohydrate calcium oxalate
Oval dumbbell
Whewellite
Triple phosphate
Magnesium ammonium phosphate
Struvite
Renal calculi may form in the
Calyces and pelvis of the kidney, ureters and bladder
Calculi with yellow to brownish red, mod. Hard
Uric acid and urate stones
Calculi with pale and friable
phosphate stones
Calculi with very hard, dark color, rough surface
Calcium oxalate stones
Calculi with yellow-brow resembling an old soap , somewhat greasy
Cystine stones
Test for mucopolysaccharide, (+) white turbidity
CTAB
Cetyltrimethyl ammonium bromide
Crystal seen in Fanconi’s syndrome
Cystine
Dilution of slightly Hazy CSF
1:10
Dilution for hazy CSF
1:20
Dilution of slightly cloudy CSF
1:100
Dilution of cloudy or slightly bloody CSF
1:200
Dilution of turbid or bloody CSF
1:10,000
Red, brown seminal fluid
Blood
Size of acrosomal gap
1/2 of the head, covers 2/3 of the nucleus
Most common cause of male infertility
Varicocele, hardening of the veins that drain the testes
Counts undiluted specimens
Sperms are immobilized by heating part of the specimen prior to charging the chamber
Markler counting chamber
Measurement of the size of of the head, neck and tail of the sperm using a micrometer or morphometer; not routine but recommended by WHO
Kruger’s strict criteria
Test for seminal fluid fructose should be analyzed within _____ or should be ____
2hrs; frozen
Florence test, choline uses
Iodine, potassium chloride
(+) Dark brown rhombic crystals
Barbiero’s test, spermine uses
Picric acid, trichloroacetic acid
(+) yellow leaf-shaped crystals
Normal synovial fluid color
Clear and pale yellow, does not clot
Normal value of synovial fluid
<3.5 mL
Normal synovial fluid glucose
Not more than 10 mg/dL lower than the blood value
Monosodium urate, uric acid
(-) birefringence under a compensated polarizing light
Gout
Calcium pyrophosphate
Blue (+) birefringence under a compensated polarizing light
Pseudogout
Calcium phosphate, No birefringence
Calcified cartilage degeneration, osteoarthritis
Normal appearance of pleural, peritoneal and pericardial fluid
Clear and pale yellow
Concentric striations of collagen-like materials, benign conditions
Ovarian and thyroid carcinoma
Psammoma bodies
Peritoneql fluid, bacterial peritonitis, cirrhosis
> 500 WBCs/ uL
Peritoneql lavage, blunt trauma
> 100,000 RBCs/uL
Increased in pancreatitis, gastrointestinal perforation in peritoneal fluid
amylase
Incrrased in gastrointestinal perforation in peritoneal fluid
Alkaline phosphatase
Dark green amniotic fluid
Meconium
Dark red brown amniotic fluid
Fetal death
Tube inserted through the nose
Levin tube
Tube inserted through the mouth
Rehfuss tube
Tubeless test for gastric acidity
Diagnex tubeless test, urine specimen Azure blue
Positive result for guiuac
Blue
Dluster of columnar cells, bronchial asthma
Creola bodies
Stool color for bile duct obstruction, barium sulfate
Pale yellow, white, gray stool
Stool for bile-duct obstruction, pancreatic disorders
Bulky/frothy stool
Intestinal constriction stool
Ribbon-like stool
Normal stool pzh
7-8
Normal of fecal leukocytes indicative of an invasive condition
> or equal 3 neutrophils/ hpf
Calibration of centrifuge
Every 3 month
Disinfection of centrifuge
Weekly basis
IRIS meaning
Interntional remote imging system
PDSA meaning
Plan-zdo-Study-Act
PDCA meaning
Plan-Do-Check-Act
Blue quadrant
Health
Red quadrant
Fire
Yellow quadrant
Reactivity
White quadrant
Specific hazard