CC Flashcards
Assay for urea that is inexpensive but lacks specificity
Colorimetric, diacetyl
Assay for urea that measures ammonia formation
Enzymatic
Simple nonspecific method for creatinine
Colorimetric, endpoint
Assay for creatinine which is rapid and with increased specificity
Colorimetric, kinetic
Assay for creatinine that measures ammonia colorimetrically or with ion-selective electrode
Enymatic
Assay for uric acid, problems with turbidity
Colorimetric
Assay for uric acid that needs special instrumentation and optical cells
Enzymatic: UV
Assay for Uric acid, interference by reducing substances
Enymatic: H2O2
Least interference with analysis
Heparin
Heparin for most chemistry tests
Lithium heparin
Glucose is metabolized at room temperature
7 mg/dL/hour
Glucose is metabolized at 4 degree celsius
2 mg/dL/hour
Ingest at least 150 g/day of carbohydrates or the 3 days preceding the test
OGTT
Performed routinely to monitor glucose control
Glycosylated hemoglobin
Gestation diabetes patients develop diabetes
Within 5 to 10 years
NCEP guidelines for acceptable measurement error for cholesterol
CV less than or equal to 3%
NCEP guidelines for acceptable measurement error for triglycerides
CV is less than or equal 5%
NCEP guidelines for acceptable measurement error of LDL, HDL
CV less than or equal to 4%
Minor lipoproteins
IDL and Lp (a)
Major structural protein in HDL
Apo A1
Major structural protein in VLDL and LDL
Apo B100
Structural protein in chylomicrons
Apo B48
LDL cholesterol may be calculated from measurement of
TC, TAG and HDL-c
Floating beta-lipoprotein
B-VLDL
Sinking pre-beta lipoprotein
Lp (a)
HDL cholesterol protective against heart disease
> 60 mg/dL
HDL cholesterol major risk for heart disease
<40 mg/dL
Serum cholesterol moderate and high risk for ages 2-19 years old
Mod: >170 mg/dL
High: >185 mg/dL
Serum cholesterol moderate and high risk for ages 20-29 years old
Mod: >200 mg/dL
High: >220 mg/dL
Serum cholesterol moderate and high risk for ages 30-39 years old
Mod: >220 mg/dL
High: >240 mg/dL
Serum cholesterol moderate and high risk for ages 40 and over
Mod: >240 mg/dL
High: >260 mg/dL