Microbiology Flashcards
What type of bacteria is Mycobacterium Tuberculosis?
Gram -Positive Acid Fast
What is the culture medium used for TB?
Lowenstein Jensen(LJ ) medium
Middlebrook 7H10, 7H11 or 7H12
What is the function of NaOH used in the Lowenstein Jensen culture for TB?
Digestion and decontamination
What substances are used for homogenization of culture in the Lowenstein Jensen culture for TB?
Acetylcysteine and NaOH
What is the condition for inoculated culture media for the Lowenstein Jensen culture for TB?
Incubated at 35 degrees Celsius for 3-6 weeks in 5-10% CO2
What group and species of mycobacteria produces yellow to orange pigment INDEPENDENT of light?
Group:Scrotochromogens
Species: M. scrofulaceum
What group and species of mycobacteria produces bright yellow pigment DEPENDENT on light?
Group: Photochromogens
Species: M. kansasii
What group and species of mycobacteria DOES NOT produce any pigment?
Group: Non-chromogens
Species: M. avium intracellulare
What are other ways of detecting TB?
- Guinea pig inoculation
- BACTEC radiometric system
(Allows detection of Mycobacterial growth in 4 – 14 days) - Nucleic acid amplification
- Polymerase Chain Reaction
What organisms may use Vincent’s Angina?
Borrelia vincenti & anaerobic fusiform bacilli
What is the most common cause of Acute streptococcal pharyngitis?
Group A streptococcus
What stain can be used to identify Diphtheria?
Albert’s stain
Which culture is used for Diphtheria?
Loeffler’s serum, Chocolate/cysteine tellurite medium
What type of bacteria is Corynebacterium
diphtheria?
Gram positive bacteria
When using the Albert’s stain , which bacteria presents with a Chinese letter like appearance?
Corynebacterium diphtheriae
What bacteria causes the disease Whooping Caught( Pertussis) often seen in children?
Bordetella pertussis
Which bacteria is associated with a fried egg appearance on culture?
Mycoplasma pneumonia
What are the symptoms associated with Atypical pneumonia?
I.P = 1 to 4 weeks
Persistent headache
Malaise
Low grade fever
Muscle aches
Sore throat
Ear pain
Nonproductive cough with substernal discomfort
Anorexia
Nausea
vomiting
What are the most common bacterial agents to cause Atypical pneumonia?
Mycoplasma pneumoniae
Chlamydia pneumoniae
Coxiella burnetti
Chlamydia psittaci
Legionella pneumophila
What are the most common viral agents present in Atypical pneumonia?
Influenza A & B
Adenovirus types 3,4 &7
Parainfluenzae virus
Respiratory syncytial virus
Varicella-zoster virus
What are the clinical features associated with Respiratory Syncytial Virus?
Nonspecific URTI
Rhinorrhea,fever,cough,wheezing.
LRTI
Tachycardia,tachypnoea,hyperinflation
What is the treatment for Actypical pneumonias caused by Mycoplasma pneumonia?
Erythromycin
Tetracycline
Doxycycline
Clarithromycin
Ofloxacin /Ciprofloxacin
Azithromycin
Erythromycin(kids under 8)
Which bacteria is transmitted by inhalation of dried bird excrement, less by bites &beak to mouth contact >
Chlamydia psittaci
