Microbiology Flashcards
- The aseptic collection of blood cultures requires that the skin be cleansed with:
A. 2% iodine and then 70% alcohol solution
B. 70% alcohol and then 2% iodine or an iodophor
C. 70% alcohol and then 95% alcohol
D. 95% alcohol only
B. 70% alcohol and then 2% iodine or an iodophor
Which of the following systems is used to remove antibiotics from body fluids that have been sent to the laboratory for bacteriologic culture?
A. ARD system
B. Roll tube system
C. Isolator system
D. API-ZYM system
A. ARD system (Antimicrobial Removal Device System)
The bacterial cell wall is composed of:
A. N-acetylglucosamine
B. N-acetylmuramic acid
C. Both A and B
D. Neither A and B
C. Both A and B
The other name for bacterial cell wall
Peptidoglycan/murein
Selective and differential media for Vibrio spp.
C. APW
A. TCBS
B. MAC
D. CCFA
TCBS - Thiosulfate Citrate Bile Salt
Enrichment media for vibrio spp.
C. APW
A. TCBS
B. MAC
D. CCFA
Alkaline Peptone water
Thiosulfate Citrate Bile Salt differentiates sucrose and non sucrose fermenters by having what indicator
Bronthymol blue
Sucrose fermenter colors
A. Blue
B. Yellow
C. Pink
D. Green
B. Yellow
Non-Sucrose Fermenters Color
A. Blue
B. Yellow
C. Pink
D. Green
D. Green
- The broth culture suspension of the test organism for use in a tube dilution is adjusted to match the turbidity of a:
A. 0.1 McFarland standard
B. 0.2 McFarland standard
C. 0.4 McFarland standard
D. 0.5 McFarland standard
- The Voges-Proskauer (VP) test detects which and product of glucose fermentation?
A. H2S
B) Acetoin
C. Tryptophanase
D. Mixed acids
B) Acetoin
Which of the following statements are correct?
- // Sterilization is a process that kills all forms of microbial life, including bacterial spores
II. Disinfection is a process that destroys pathogenic organisms, but not necessarily all microorganisms or spores.
III. Incineration is the most common method of treating infectious waste. IV. Moist heat (steam under pressure) is used to sterilize biohazardous trash and heat-stable objects
A. I and II
B. I and III
C. I, II, III
D. I, II, III, IV
D. I, II, III, IV
The following are methods of sterilization except:
A. Boiling
B. Moist heat
C. Dry Heat
D. Filtration
A. Boiling
100 deg Celcius - 15 minutes
WHAT COLOR Lactose fermenters on MAC
A. Blue
B. Yellow
C. Pink
D. Green
C. Pink
Select the incorrect match between the organism and appropriate culture media:
A. CCFA-Clostridium difficile
B. Tomato Juice Agar - Lactobacillus
C. Regan-Lowe Agar - Bordetella
D. CNA Agar - Neisseria
D. CNA Agar - Neisseria
GRAM NEGATIVE COCCI EXCEPT:
A. Neisseria
B. Veilonella
C. Moraxella
D. Bordetella
D. Bordetella
What step in the Gram stain distinguishes between gram-positive and gram-negative organisms?
A. Fixing of the cells to the slide using heat or methanol
B. Decolorization using alcohol or acetone
C. Counterstain of the Gram stain using safranin
D. Application of the mordant, Gram’s iodine
B. Decolorization using alcohol or acetone
- Electron microscopy allows for what magnification of an organism?
A. 1000×
B. 10000×
C. 100000×
D. 1000000×
C. 100000×
Light microscopy allows for what magnification of an organism?
A. 1000×
B. 10000×
C. 100000×
D. 1000000×
A. 1000×
At which pH does the methyl red (MR) test become positive?
A. 7.0
B. 6.5
C. 6.0
D. 4.5.
D. 4.5
- The two cations known to influence the activity of aminoglycosides are:
A. Sodium and potassium
B. Calcium and potassium
C. Calcium and magnesium
D. Sodium and magnesium
C. Calcium and magnesium
Ca= 25 mg/L
Mg= 12.5 mg/L
Which of these antimicrobial agents inhibits DNA synthesis?
A. Fluoroquinolones
B. Beta-lactam agents
C. Aminoglycosides
D. Glycopeptides
A. Fluoroquinolones
Which of these antimicrobial agents inhibits cell wall?
A. Fluoroquinolones
B. Beta-lactam agents
C. Aminoglycosides
D. Glycopeptides
B. Beta-lactam agents
Which of these antimicrobial agents inhibits 30s proteins?
A. Fluoroquinolones
B. Beta-lactam agents
C. Aminoglycosides
D. Glycopeptides
C. Aminoglycosides
