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MTAP 1st day Compre Reviewer > Microbiology > Flashcards

Microbiology Flashcards

1
Q
  1. The aseptic collection of blood cultures requires that the skin be cleansed with:

A. 2% iodine and then 70% alcohol solution

B. 70% alcohol and then 2% iodine or an iodophor

C. 70% alcohol and then 95% alcohol

D. 95% alcohol only

A

B. 70% alcohol and then 2% iodine or an iodophor

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2
Q

Which of the following systems is used to remove antibiotics from body fluids that have been sent to the laboratory for bacteriologic culture?

A. ARD system

B. Roll tube system

C. Isolator system

D. API-ZYM system

A

A. ARD system (Antimicrobial Removal Device System)

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3
Q

The bacterial cell wall is composed of:

A. N-acetylglucosamine

B. N-acetylmuramic acid

C. Both A and B

D. Neither A and B

A

C. Both A and B

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4
Q

The other name for bacterial cell wall

A

Peptidoglycan/murein

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5
Q

Selective and differential media for Vibrio spp.

C. APW

A. TCBS

B. MAC

D. CCFA

A

TCBS - Thiosulfate Citrate Bile Salt

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6
Q

Enrichment media for vibrio spp.

C. APW

A. TCBS

B. MAC

D. CCFA

A

Alkaline Peptone water

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7
Q

Thiosulfate Citrate Bile Salt differentiates sucrose and non sucrose fermenters by having what indicator

A

Bronthymol blue

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8
Q

Sucrose fermenter colors

A. Blue

B. Yellow

C. Pink

D. Green

A

B. Yellow

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9
Q

Non-Sucrose Fermenters Color

A. Blue

B. Yellow

C. Pink

D. Green

A

D. Green

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10
Q
  1. The broth culture suspension of the test organism for use in a tube dilution is adjusted to match the turbidity of a:

A. 0.1 McFarland standard

B. 0.2 McFarland standard

C. 0.4 McFarland standard

D. 0.5 McFarland standard

A
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11
Q
  1. The Voges-Proskauer (VP) test detects which and product of glucose fermentation?

A. H2S

B) Acetoin

C. Tryptophanase

D. Mixed acids

A

B) Acetoin

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12
Q

Which of the following statements are correct?

  1. // Sterilization is a process that kills all forms of microbial life, including bacterial spores

II. Disinfection is a process that destroys pathogenic organisms, but not necessarily all microorganisms or spores.

III. Incineration is the most common method of treating infectious waste. IV. Moist heat (steam under pressure) is used to sterilize biohazardous trash and heat-stable objects

A. I and II

B. I and III

C. I, II, III

D. I, II, III, IV

A

D. I, II, III, IV

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13
Q

The following are methods of sterilization except:

A. Boiling

B. Moist heat

C. Dry Heat

D. Filtration

A

A. Boiling
100 deg Celcius - 15 minutes

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14
Q

WHAT COLOR Lactose fermenters on MAC

A. Blue

B. Yellow

C. Pink

D. Green

A

C. Pink

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15
Q

Select the incorrect match between the organism and appropriate culture media:

A. CCFA-Clostridium difficile

B. Tomato Juice Agar - Lactobacillus

C. Regan-Lowe Agar - Bordetella

D. CNA Agar - Neisseria

A

D. CNA Agar - Neisseria

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16
Q

GRAM NEGATIVE COCCI EXCEPT:

A. Neisseria

B. Veilonella

C. Moraxella

D. Bordetella

A

D. Bordetella

17
Q

What step in the Gram stain distinguishes between gram-positive and gram-negative organisms?

A. Fixing of the cells to the slide using heat or methanol

B. Decolorization using alcohol or acetone

C. Counterstain of the Gram stain using safranin

D. Application of the mordant, Gram’s iodine

A

B. Decolorization using alcohol or acetone

18
Q
  1. Electron microscopy allows for what magnification of an organism?

A. 1000×

B. 10000×

C. 100000×

D. 1000000×

A

C. 100000×

19
Q

Light microscopy allows for what magnification of an organism?

A. 1000×

B. 10000×

C. 100000×

D. 1000000×

A

A. 1000×

20
Q

At which pH does the methyl red (MR) test become positive?

A. 7.0

B. 6.5

C. 6.0

D. 4.5.

A

D. 4.5

21
Q
  1. The two cations known to influence the activity of aminoglycosides are:

A. Sodium and potassium

B. Calcium and potassium

C. Calcium and magnesium

D. Sodium and magnesium

A

C. Calcium and magnesium

Ca= 25 mg/L
Mg= 12.5 mg/L

22
Q

Which of these antimicrobial agents inhibits DNA synthesis?

A. Fluoroquinolones

B. Beta-lactam agents

C. Aminoglycosides

D. Glycopeptides

A

A. Fluoroquinolones

23
Q

Which of these antimicrobial agents inhibits cell wall?

A. Fluoroquinolones

B. Beta-lactam agents

C. Aminoglycosides

D. Glycopeptides

A

B. Beta-lactam agents

24
Q

Which of these antimicrobial agents inhibits 30s proteins?

A. Fluoroquinolones

B. Beta-lactam agents

C. Aminoglycosides

D. Glycopeptides

A

C. Aminoglycosides

25
Q
  1. The mechanism of action for tetracycline is inhibition of:

A. RNA synthesis

B. Cell wall synthesis

C. Protein synthesis

D. Membrane function

A

C. Protein synthesis

Inhibits 30s and 50s

26
Q

Blood agar plates are ideally prepared with 5% RBCs obtained from:

A. Sheep

B. Horses

C. Humans

D. Dogs

A

A. Sheep

27
Q
  1. What CO2 concentration is achieved when using a candle jar?

A. 1%

B. 3%

C. 6%

D.9%

A

B. 3%

28
Q

Select the method of choice for recovery of anaerobic bacteria from a deep abscess.

A. Cotton fiber swab of the abscess area

B. Skin snip of the surface tissue

C.Needle aspirate after surface decontamination

D. Swab of the scalpel used for débridement

A

C.Needle aspirate after surface decontamination

29
Q

All of the following are appropriate when attempting to isolate N. gonorrhoeae from a genital specimen except:

A. Transport the genital swab in charcoal transport medium

B. Plate the specimen on modified Thayer-Martin (MTM) medium

C. Plate the specimen on New York City or Martin-Lewis agar

D. Culture specimens in ambient oxygen at 37°C

A

D. Culture specimens in ambient oxygen at 37°C

Neisseria must incubate in a CO2 environment -CATHOPHILES

30
Q

Differential enrichment medium for C. neoformans (brown colonies due to phenol oxidase)

A

Birdseed Agar/Staib

31
Q

For isolation of pathogenic yeast and dimorphic fungi in yeast form

A

Brain-Heart Infusion Agar (BHI)

32
Q

Differential medium, Cryptococcus neoformans (poor growth; greenish yellow) VS Cryptococcus gotti (good growth; cobalt blue)

A

Canavanine-Glycine- Bromthymol Blue Agar

33
Q

For conidia and chlamydospore production of C. albicans Tween 80 enhances chlamydospore production

A

Cornmeal Agar

34
Q

provides conversion of dimorphic Blastomyces dermatitidis from mold to yeast

A

Cottonseed medium

35
Q

Isolation of dermatophytes from cutaneous specimens
Indicator: phenol red

A

Dermatophyte Test medium

36
Q

Enriched selective medium for growth of cycloheximide-sensitive fungi
(Cryptococcus, H. capsulatum, and mucoraceous fungi)

A

Inhibitory mold agar

37
Q

General purpose medium used to isolate fungi, especially dermatophytes
Similar to inhibitory mold agar in that it does not contain cycloheximide

A

Littman Oxgall agar

38
Q

Differential medium useful to assess ability of yeast to assimilate KNO3
Nitrate (-): C. neoformans, C. albicans; Nitrate (+): C. albidus

A

Modified Potassium Nitrate Assimilation medium

MTAP 1st day Compre Reviewer (10 decks)

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