Histopath Flashcards
Fix the tissue by immersing it in:
a. ascending alcohol concentration
b. descending alcohol concentration
c. neither
a. ascending alcohol concentration
*70% - 15 min;
95% - 15 min;
100% 30 min
Tissues are group of cells of common origin and function. They are derived
from three primitive germ layers. Which of the following pairs of primary
tissue-germ layer is correct?
a. Connective –mesoderm
b. Muscular – mesoderm
c. Nervous – ectoderm
d. All of these
e. None of these
a. Connective –mesoderm
Pathology came from the ancient Greek word “pathos” meaning:
a. Study of Death
b. Suffering
c. To set on fire
d. None of these
b. Suffering
The following pairs of cardinal sign-meaning are correct. EXCEPT
a. Tumor – swelling
b. Calor – heat
c. Dolor – pain
d. Functio laesa – well functioning
d. Functio laesa – well functioning
This term refers to the macrophage seen in the liver:
a. Kupffer cells
b. Histiocytes
c. Microglia
d. Pulmonary alveolar macrophage
a. Kupffer cells
NOT TRUE regarding apoptosis:
a. A programmed cell death
b. A normal phenomenon that serves to eliminate cells that are no longer needed and
to maintain a steady number of various cell populations in tissues
c. Also a pathologic event when cells are damaged beyond repair, especially when the
damage affects the cell’s DNA or proteins
d. The same as necrosis
e. None of these
d. The same as necrosis
The first demonstrable secondary change after death is:
a. Circulatory failure
b. Stiffening of the muscles
c. Post-mortem lividity
d. Cooling of the body
d. Cooling of the body
Fat stain that has greatest affinity to phospholipids among all the fat stains
and gives triglycerides black color:
a. Sudan black B
b. Scharlach R
c. Sudan III
d. Sudan IV
a. Sudan black B
Mounting mediums, EXCEPT:
a. DPX
b. XAM
c. Clarite
d. Option 4
e. Poly-L-lysine
e. Poly-L-lysine
Plane concave knife:
a. Is usually 25mm in length
b. Is usually 100mm in length
c. Is usually 120mm in length
d. None of the above
a. Is usually 25mm in length
The volume of the impregnating medium should be at least _____ the volume of the tissue
a. 5x
b. 10x
c. 25x
d. 50x
c. 25x
Which of the following is the correct order of the steps in tissue processing?
1 – decalcification
2 – fixation
3 – impregnation
4 – clearing
5 – dehydration
a. 2,5,1,4,3
b. 2,1,5,4,3
c. 1,2,5,4,3
d. 1,5,2,4,3
e. none
b. 2,1,5,4,3
The use of mercuric chloride as fixative may lead to the formation of
______________ colored deposits.
a. Yellow
b. White
c. Silver
d. Black
d. Black
Sensitizers
a. Are substances that cause destruction or irreversible alteration when exposed to
living tissue, or destroy inanimate surfaces
b. Cause allergic reactions in substantial proportion of exposed subjects not just in
hypersensitive individuals
c. Substances that induce tumors, not only in experimental animals but also in humans
d. Are capable of causing death upon ingestion, skin contact or inhalation at certain
specified concentrations
b. Cause allergic reactions in substantial proportion of exposed subjects not just in
hypersensitive individuals
In the washing out procedure to remove the excess fixative from the tissue
after fixation, tap water:
a. Can be used to remove excess chromate from tissue fixed with Helly’s Zenker’s and
Flemming’s solution
b. Can be used to remove osmic acid
c. Can never be used for washing out procedures
d. Both A and B
d. Both A and B
Process whereby selected tissue is immersed in a watch glass containing
isotonic salt solution, carefully dissected or separated and examined under
microscope
a. Teasing
b. Squash preparation
c. Streaking
d. Spreading
a. Teasing
Hypertrophy:
a. Refers to an increase in size of tissues or organs due to increase in the size of the
individual cells
b. Refers to an increase in the size of an organ or tissue due to increase in the number
of cells resulting from growth of new cells
c. Reversible transformation from one type of adult cell into another
d. Continuous abnormal proliferation of cells without control, serving no useful
function, with accompanied increase in size and pigmentation
a. Refers to an increase in size of tissues or organs due to increase in the size of the
individual cells
Which of the following is the best fixative for exfoliative cytology?
a. Ether alcohol
b. 50% alcohol solution
c. 10% formalin
d. 95% ethyl alcohol
d. 95% ethyl alcohol
Cardinal sign of inflammation that occurs due to transfer of internal heat to
the surface site of injury, brought by increased blood content:
a. Rubor
b. Calor
c. Tumor
d. Dolor
b. Calor
Nuclear stains:
a. Methylene blue
b. Celestine blue
c. Toluidine blue
d. All of these
d. All of these
A stain used in the peripheral blood staining to differentiate leukocytes is:
a. Masson stain
b. Celestine blue
c. Giemsa stain
d. Malachite green
c. Giemsa stain
Chromate fixative include:
a. Bouin’s fluid
b. Zenker’s fluid
c. Moller’s fluid
d. Both A and C
c. Moller’s fluid
The rate of tissue fixation can be retarded by which of the following
parameters
a. Presence of blood
b. Presence of mucus
c. Agitation
d. Both A and B
d. Both A and B
An ideal fixative is all of these, except:
a. Inexpensive
b. Penetrates tissue slowly
c. Preserves tissue elements
d. Prevents autolysis
b. Penetrates tissue slowly
Optimum temperature range and the recommended ratio of fluid-to-tissue
during decalcification is:
a. 18C to 30C; 20:1
b. 19C to 30C; 10:1
c. 30C to 37C; 20:1
d. 30 to 37C; 10:1
a. 18C to 30C; 20:1
Post decalcification treatment of tissue is important to remove excess acid
from the tissues. This can be accomplished by immersing the tissues into:
a. Saturated lithium carbonate
b. 5% to 10% aqueous sodium bicarbonate solution
c. Running to tap water
d. All of these
d. All of these
A combination of basic fuchsin and phenol:
a. Schiff
b. Mallory’s fuchsin
c. Aldehyde fuchsin
d. Carbol fuchsin
d. Carbol fuchsin
Acts both as dehydrating and clearing agent:
a. Ethylene glycol monoethyl ether
b. Tetrahydrofuran
c. Triethytl phosphate
d. Both A and B
b. Tetrahydrofuran
Which of the following acts as a clearing agent?
a. Acetone
b. Carbon tetrachloride
c. Cellosolve
d. Both B and C
b. Carbon tetrachloride
Paldwell Trefall invented which of the following microtome types?
a. Base sledge type
b. Cambridge type
c. Rotary type
d. None of these
b. Cambridge type
