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MICROBIO W1-DAY3_ALN Flashcards

1
Q

Test for ID of gram(+) organisms

A

Catalase test

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2
Q

Catalase +/-:

Staphylococci

A

Catalase +

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3
Q

Catalase +/-:

Streptococci

A

Catalase -

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4
Q

Catalase +/-:

Enterococci

A

Catalase -

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5
Q

Catalase +/-:

Listeria monocytogenes

A

Catalase +

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6
Q

Catalase +/-:

Corynebacteria

A

Catalase +

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7
Q

Test initially used for differentiating between groups of Gram (-)

A

Oxidase test

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8
Q

Gram (-) Oxidase (-) bacilli

A

ESA

Enterobacteriaceae
Stenotrophomonas maltophilia
Acinetobacter spp.

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9
Q

Gram (-) Oxidase (+) bacilli

A

PA

Pseudomonas spp.
Aeromonas spp.

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10
Q

Indole test detects which enzyme?

A

Tryptophanase

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11
Q

OXIDASE REACTION

Vibrio:
Enterobacteriaceae:

A

Vibrio: Oxidase +
Enterobacteriaceae: Oxidase -

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12
Q

Indole test presumptively identifies this bacteria

A

E. coli

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13
Q

E. coli IMVC rxn

A

+ + - -

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14
Q

INDOLE TEST RXN

E.coli

A

+

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15
Q

UREASE TEST (+) ENTEROBACTERIACEAE

A

PPM

Proteus
Providencia
Morganella

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16
Q

Key enzyme of H. pylori

A

Urease

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17
Q

Gram - bacilli associated with peptic ulcer

A

H. pylori

NOTE:
Strong producer of urease that converts urea to ammonia which neutralized acid pH

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18
Q

PYRase (+) Streptococcus group

A

Group A (S. pyogenes)

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19
Q

PYRase (+) Enterococcus grp

A

Group D

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20
Q

The only hippurate hydrolysis (+) Campylobacter

A

C. jejuni

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21
Q

Hippurate Hydrolysis (+)

A

SLC-G

S. agalactiae
L. monocytogenes
C. jejuni
G. vaginalis

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22
Q

Common among S. agalactiae, L. monocytogenes, C. jejuni, G. vaginalis

A

Hippurate Hydrolysis +

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23
Q

Planococcus and Stomatococcus are Micrococcaceae genera that can be found among (human/animals).

A

Animals

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24
Q

Grape-like cocci (in clusters)

A

Staphylocci

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25
Q

Gram + micrococcaceae in tetrads or in sarcinae

A

Micrococcus

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26
Q

BAP contains ___% defibrinated (sheep/horse) blood

A

5%; sheep

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27
Q

Preferred types of defibrinated blood in BAP (Descending order)

A

1: sheep
2: horse
3: rabbit

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28
Q

Type of blood used to demonstrate B-hemolytic Haemophilus

A

Horse

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29
Q

Type of blood used to demonstrate B-hemolytic Haemophilus

A

Horse

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30
Q

Non-specific inhibitors found in Human Blood (not preferred for BAP)

A

Citrate
Dextrose

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31
Q

Staph/Strep:

Pinhead colonies

A

Staph

NOTE: Staph has larger colonies than Strep.

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32
Q

Staph/Strep:

Pinpoint colonies

A

Strep

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33
Q

MSA inhibitor

A

7.5-10% NaCl

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34
Q

Gram + cocci capable of growing in presence of 7.5% NaCl

A

Staphylococci (Halophilic)

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35
Q

MSA differentiates Staphylocci based on the fermentation of what carb alcohol?

A

Mannitol

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36
Q

Incomplete hemolysis on BAP

37
Q

Identify hemolytic pattern on BAP:

Produces green or brown area around colony

A

Alpha (Incomplete)

38
Q

Complete hemolysis

A

Beta hemolysis

39
Q

Identify hemolytic pattern on BAP:

Clear area around colony

A

Beta (complete)

40
Q

Identify hemolytic pattern on BAP:

No lysis of red cells around colony

41
Q

Identify hemolytic pattern on BAP:

No change in agar

42
Q

Identify hemolytic pattern on BAP:

Small zone of alpha surrounded by a zone of B hemolysis after refrigeration

A

Alpha-prime

43
Q

Identify hemolytic pattern on BAP:

Surrounded by two zones of hemolysis

A

Alpha-prime

Inner: alpha
Outer: Beta

44
Q

TRUE or FALSE:

Alpha prime pertains to double zone of hemoysis of C. perfringens.

A

FALSE

C. perfringens: Inner = beta; outer = alpha

45
Q

Golden yellow colonies on LSS

46
Q

Lemon yellow colonies on LSS

A

S. citreus

47
Q

Porcelain white colonies on LSS

A

S. albus (S. epidermidis)

48
Q

MSA pH indicator

A

phenol red

49
Q

S. aureus on MSA

A

yellow (MF)

50
Q

S. epidermidis (albus) on MSA

A

pink (Non-M F)

51
Q

S. saprophyticus on MSA

A

variable (pink/yellow)

52
Q

What should a culture medium contain to be labeled as “selective”?

53
Q

What should a culture medium contain to be “differential?”

54
Q

TSI pH indicator

A

phenol red

55
Q

Urease medium pH indicator

A

phenol red

56
Q

Phenol red at an acidic pH

57
Q

Phenol red at an alkaline pH

58
Q

Main test to differentiate Staph vs. Strep

59
Q

catalase test rgt

A

3% hydrogen peroxide

60
Q

positive result for catalase test

A

effervescence, bubbles

61
Q

Test to differentiate Staphylococcus vs Micrococcus

A

Modified Oxidase Test

62
Q

Staph vs. Micro

Lysostaphin susceptible

63
Q

Staph vs. Micro

Furazolidone susceptible

64
Q

Staph vs. Micro

Lysostaphin resistant

65
Q

Staph vs. Micro

Furazolidone resistant

66
Q

Staph vs. Micro

Bacitracin resistant

67
Q

Staph vs. Micro

Bacitracin susceptible

68
Q

Staph vs. Micro

Mod. Oxidase +

69
Q

Staph vs. Micro

Mod. Oxidase -

70
Q

Staph glucose utilization

71
Q

Micrococcus glucose utilization

72
Q

Gram + cocci that ferments glucose

73
Q

Microdase test + reaction for gram + cocci

74
Q

Modified Oxidase test rgt

A

Tetramethyle paraphenylene diamine dihydrochloride w/ dimethylsulfoxide (DMSO)

75
Q

Original oxidase (for Neisseria) + reaction

A

Purple color

76
Q

Glucose utilization test is also known as:

A

Oxidation-Fermentation (OF) test

77
Q

OF test medium

A

OF tube with incorporated glucose

78
Q

CHO in OF test

79
Q

OF test pH indicator

A

Bromthymol blue

80
Q

OF test for gram + cocci:

Open tube (+) Closed tube (-)

A

Micrococcus

81
Q

OF test for gram + cocci:

Open tube (+) Closed tube (+)

A

Staphyloccus

82
Q

In OF test, closed tube is sealed with?

A

Mineral oil or Vaseline with parafilm (VASPAR)

83
Q

Common pH indicators used for OF test

A

Bromcresol purple (purple to yellow)
Andrade’s Acid Fuchsin Indicator (pale yellow to pink)
Phenol Red (red to yellow)
Bromthymol blue (green to yellow)

84
Q

The mechanism of Andrade’s Acid Fuchsin indicator is similar to what pH indicator?

A

Neutral red

A: red to pink
K: red to yellow

85
Q

Single most important pathogenic determinant of S. aureus

A

Coagulase Test

(S. aureus vs. Coagulase - Staph)

86
Q

Coagulase test rgt

A

Rabbit’s plasma

87
Q

Anticoagulant of choice in using human plasma for coagulase test

A

EDTA

NOTE: Citrate = false (+)

88
Q

Tests used to differentiate coagulase (+) staphylococci

A

Voges-Proskauer test
PYR test

89
Q

Test to differentiate S. epidermidis vs. S saprophyticus

A

Novobiocin Resistance test

01LMR_MOTHER NOTES (11 decks)

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