01SUSHI_MICROBIO Flashcards
Gram + aerobic cocci
[SMS]
Staphylococcus
Micrococcus
Streptococcus
Gram - aerobic cocci
[NM]
Neisseria
Moraxella
Gram + anaerobic cocci
[SPP]
Sarcina
Peptococcus
Peptostreptococcus
gram - anaerobic cocci
Veilonella
gram + aerobic bacilli
[BCELL - MN]
Bacillus
Corynebacterium
Erysipelothrix
Listeria
Lactobacillus
Mycobacterium
Nocardia
gram + anaerobic bacilli
[CAP]
Clostridium
Actinomyces
Propionobacterium
gram - anaerobic bacilli
[FBPP]
Fusobacterium
Bacteroides
Prevotella
Porphyromonas
MacConkey pH indicator
neutral red
SSA pH indicator
neutral red
culture media with phenol red as pH indicator
[MX CUT]
MSA
XLD
CTA
UREASE
TSI
culture media with bromthymbol blue as pH indicator
[CHOTS]
Citrate
HEA
OF Hugh Leifson
TCBS
SCA
LIA pH indicator
bromcresol purple
ideal specimen for recovery of Pneumocystis carinii
BAL
sp of choice for Aspergillus recovery
BAL
specimens that cannot be refrigerated
CSF
blood
gonorrhea swab
specimens that can be refrigerated within 1 hr at 4C
urine
sputum
stool
swab except genital swab
microorganisms that SPS inhibits
G. vaginalis
S. moniliformis
P. anaerobius
Neisseria
BACTEC 9120 TAT
5 days
CSF centrifugation setting
2000 rpm, 10 mns
Sputum centrifugation setting for TB culture
3000g for 15 mns
Leprosy specimen
Tissue juice
Stains for metachromatic granules
Burke’s modification of GS
Loeffler’s Alkaline Methylene Blue (LAMB)
Modification of GS stain used for demonstration of fungi
Hucker’s
stain and rgt used to enhance polychromatic effect in Hucker’s test
crystal violet; ammonium oxalate
decolorizer in modified AFS
1% H2SO4
counterstain in fluorescent staining for AFB
0.5% KMnO4 (quenching agent)
Preservative in trichrome stainine
PVA
B. anthracis capsule stain
McFadyean’s
Stains for capsule
Hiss
Tyler
Muir
India ink
Soil transmitted parasites
[HATS]
Hookworm
A. lumbricoides
T. trichiura
S. stercoralis
Food transmitted parasites
[TTT]
Taenia (pork/beef)
Trichinella (pig/rat)
Toxoplasma (cat)
Contact transmitted parasites
[ET]
Enterobius
Trichomonas
Parasites with Cockroach vector
Ascaris
Trichuris
Parasites with intranasal entry
[HAN]
Hartmanella
Acanthamoeba
Naegleria
Parasites that can be transmitted transmammary
Strongyloides
A. duodenale
Parasites with urine exit
T. vaginalis
S. haematobium
Parasites with sputum exit
[APEEECT]
ASH: larva
Paragonimus: egg
E. granulosus: cyst
E. histolytica: troph
E. gingivalis: trum
C. parvum: oocyst
T. tenax: troph
Diseases that can be diagnosed through muscle biopsy
Trichinosis (T. spiralis)
Sparganosis (D. latum)
Cysticercosis (T. solium)
Diseases diagnosed via rectal biopsy
Amebiasis
Schistosomiasis
Parasites with granuloma formation
[SAG]
Schistosoma
Anisakis
GAE
Aphasmid nematodes
[TCT]
Trichuris
Capillaria
Trichinella
Negative stains
India ink
Anthony’s
Stains for metachromatic granules
Alvert
Neisser
Lindergren’s
LAMB
Burke’s
Stain for bipolar bodies
Wayson stain
Stains for flagella
FLGSC
Fisher & Conn
Leifson
Gray
Silver technique
Caesares Gil
Stains for endospores
Dorner’s
Schaeffer Fulton’s
Wirtz Conklin
Stain for spirochetes
[FL]
Fontana Tribondeau
Levaditi silver impregnation
Stain for mycoplasma
Dienes
Stain for cell wall
Victoria blue
Stain for DNA
Feulgen
Stain for acid fast bacilli
Ziehl Neelsen
Kinyoun
Pappenheim’s (TB red SMEG blue)
Baumgarten’s (TB blue LEPRAE red)
Wadefite
Protozoan staining using iodine
Troph:
Chromatoidal bodies:
Cyst cytoplasm:
Troph: Destroyed
Chromatoidal bodies: Unstained
Cyst cytoplasm: Yellow brown
Glycogen color using trichrome
colorless
Chromatoidal bodies color using trichrome stain
bright red
Parasite nucleus color using trichrome
red-purple
Charcot-Leyden color using trichrome
bright-red
Excessive concentrations of ____________ can result in false resistance to sulfonamides and trimethoprim
Thymidine
Decreased pH effect on aminoglycosides and tetracycline
low pH = decreased activity of aminoglycosides, erythromycin, clindamycin and increased activity of tetracycline
Biphasic medium for Brucella blood culture
Castaneda
Media for screening suspected cases of hemorrhagic E. coli O157:H7 must contain
Sorbitol
N. gonorrhoeae and N. meningitidis both grown in CAP. Which of the two will grow on BAP?
N. meningitidis
AST media for Neisseria
GC agar
Carbohydrate fermentation media for Neisseria
Cysteine trypticase agar + phenol red
Transport media for N. meningitidis
Amies with charcoal
Which of the following culture media are clear?
GC
Modified TMA
Martin Lewis
New York City
New York city (yeast extract based)
Others: CAP based
Corynebacterium appearance in tellurite:
gray-black
Corynebacterium appearance in tinsdale
black with brown halo
Corynebacterium appearance in cystine tellurite:
gun-metal gray
Small intestine inhabiting nematodes
CASH
Capillaria
Ascaris
Strongyloides
Hookworm
Large intestine inhabiting nematodes
[ET]
Enterobius
Trichuris
Nematodes in Meninges
Angiostrongylus (eosinophilic ME)
Naegleria (PAM)
Acanthamoeba (GAE)
Most frequently isolated nematodes
ATE
Ascaris
Trichuris
Enterobius
3 layers of Ascaris egg
AGV
Albuminoid
Glycogen
Vitelline
Parasites capable of autoinfection
CHEST
Capillaria
Hymenolepis nana
Enterobius
Strongyloides
Taenia solium
Parasites with heart-lung migration
ASH
Ascaris (loeffler’s)
Strongyloides (Loeffler’s)
Hookworm (Wakana)
Parasites that utilize xenodiagnosis
TsTc
Trichinella spiralis
Trypanosoma cruzi
Visceral larva migrans
Toxocara canis
Cutaneous larva migrans
Hookworm
Bachmann Intradermal
Trichinella
Casoni skin test
Echinococcus
COLONY APPEARANCE:
cauliflower
M. tuberculosis
COLONY APPEARANCE:
coral red fluorescence on Wood’s light with porphyrin
C minutissimum
COLONY APPEARANCE:
dew drop
Haemophilus
COLONY APPEARANCE:
golden (better at 25-30)
Staphylococcus
COLONY APPEARANCE:
hockey puck
Moraxella
COLONY APPEARANCE:
molar tooth
A. israelii
COLONY APPEARANCE:
Nonpigmented
Streptococcus
COLONY APPEARANCE:
Snow flake on BG agar
Salmonella
COLONY APPEARANCE:
string of pearls (media with penicillin)
B. anthracis
COLONY APPEARANCE:
Bamboo pole, medusa head
B. anthracis
COLONY APPEARANCE:
Wrinkled
N. sicca
MOTILITY
Corkscrew
Spirochetes (axial filaments)
MOTILITY
Darting
Campylobacter
MOTILITY
Gliding
Capnocytophaga
Mycoplasma pnuemoniae
MOTILITY
Rapid darting/shooting star
Vibrio
MOTILITY
Spinning
Leptospira
MOTILITY
Tumbling
Listeria
MOTILITY
Twitching
Kingella
Bartonella
ASSOCIATED DISEASE:
Lumpy jaw
A. israelii
ASSOCIATED DISEASE:
Black eschar
B. anthracis
ASSOCIATED DISEASE:
Ragpicker’s/Woolsorter’s
Gastroenteritis and bloody diarrhea
B. anthracis