01SUSHI_CC Flashcards
Conversion Factor:
Creatinine
88.4
Conversion Factor:
Bilirubin
17.1
Conversion Factor:
T4
12.9
Conversion Factor:
Albumin
10
Conversion Factor:
Hemoglobin
10
Conversion Factor:
Total Protein
10
Conversion Factor:
Folic acid
2.27
Conversion Factor:
Bicarbonate
1
Conversion Factor:
Chloride
1
Conversion Factor:
Lithium
1
Conversion Factor:
Osmolality
1
Conversion Factor:
Potassium
1
Conversion Factor:
Sodium
1
Conversion Factor:
NH3
0.587
Conversion Factor:
Mg
0.5
Conversion Factor:
BUN
0.357
Conversion Factor:
Phosphorus
0.323
Conversion Factor:
Calcium
0.25
Conversion Factor:
Iron
0.179
Conversion Factor:
PCO2
0.133
Conversion Factor:
PO2
0.133
Conversion Factor:
Vitamin B12
0.0738
Conversion Factor:
Uric Acid
0.0595
Conversion Factor:
Glucose
0.0555
Conversion Factor:
Cortisol
0.0276
Conversion Factor:
Cholesterol
0.026
Conversion Factor:
AST
0.0167
Conversion Factor:
Creatinine Clearance
0.0167
Conversion Factor:
Triglyceride
0.0113
Conversion Factor:
BUN CU to SI
0.357
Conversion Factor:
Urea to BUN
0.467
Conversion Factor:
BUN to Urea
2.14
Conversion Factor:
N to Protein
6.25
Steps in using a fire extinguisher
[PASS]
Pull Pin
Aim at the base of the Fire
Squeeze handles
Sweep nozzle side to side
In case of fire,
[RACE]
Rescue
Alarm
Contain
Evacuate/Extinguish
Type of Fire:
Wood, paper, clothing
A
Type of Fire:
Flammable organic chemicals
B
Type of Fire:
Electrical
C
Type of Fire:
Combustible Metals
D
Type of Fire:
Grease, Oils, Fats
K
Safety Diamond:
Blue
Health
Safety Diamond:
Red
Flammability/Fire
Safety Diamond:
Yellow
Reactivity/Instability
Safety Diamond:
White
Special/Specific
First step during repair of electronic equipment
Turn instrument off and Unplug
Systematic/Random Error?
1 2s
Random
Systematic/Random Error?
1 3s
Random
Systematic/Random Error?
R 4s
Random
Systematic/Random Error?
2 2s
Systematic
Systematic/Random Error?
4 1s
Systematic
Systematic/Random Error?
10x
Systematic
Systematic/Random Error?
Mislabeling
Random
Systematic/Random Error?
Pipetting
Random
Systematic/Random Error?
Mixing
Random
Systematic/Random Error?
Voltage and temp fluctuations
Random
Systematic/Random Error?
Improper calibration
Systematic
Systematic/Random Error?
Reagent deterioration
Systematic
Systematic/Random Error?
Sample instability
Systematic
Systematic/Random Error?
Instrument drift
Systematic
Systematic/Random Error?
Changes in standard materials
Systematic
6 or more consecutive values distributed on one side of the mean
SHIFT
Main cause of SHIFT
IMPROPER CALIBRATION
6 or more consecutive values that continue to either increase or decrease
TREND
Main cause of TREND
REAGENT DETERIORATION
Ability to detect a small quantity of analyte
Analytical Sensitivity
Ability to detect only the analyte of interest
Analytical Specificity
Positive result in the presence of disease
Diagnostic Sensitivity
Negative result in the absence of disease
Diagnostic Specificity
Provides polychromatic light in spectrophotometer
Light Source
Minimizes stray light in spectrophotometer
Entrance slit
Isolates specific wavelength in spectrophotometer
Monochromator
Controls bandpass (width of light beam) in spectrophotometer
Exit Slit
Contains solution to be measured in spectrophotometer
Cuvette
Converts light into electric energy in spectrophotometer
Photodetector
Output display in spectrophotometer
Read out device
Visible light spectrum in spectrophotometer
400-700 nm
UV region in spectrophotometer
<400 nm
Infrared region in spectrophotometer
> 700 nm
In spectrophotometry,
Exciter lamp: UV
Mercury
Xenon
Deuterium
In spectrophotometry,
Exciter lamp: Visible and infrared
Tungsten
In spectrophotometry, this is the most sensitive photodetector
Photomultiplier
Double beam in space # of photodetector
2
Double beam in time # of photodetector
1
Fluorescence reduction
Quenching
Measures amount of light scattered
Nephelometry
Measures amount of light blocked
Turbidimetry
With 2 monochromators positioned at 90 degrees
Fluorometer
In fluorometer, 2 monochromators are positioned at ______ degrees
90
Fluorescence of an analyte is reduced to the excited molecule losing some of its energy by interacting with other substances in solution
Quenching
Measures light emitted by the analyte after being excited by the flame
Flame Emission Photometry (FEP)
Reference method for Ca and Mg
Atomic Absorption Spectometry (AAS)
Disinfection of centrifuge is done _________.
Weekly
Calibration of centrifuge is done __________.
Quarterly
Horizontal Centrifuge is also known as
Swinging bucket
Angle-Head centrifuge is also known as:
Fixed-angle centrifuge
Analytes affected by hemolysis
[PM5LICCK]
Phosphate
Mg
Ammonia, ALT, AST, ACP, ALP
LD (LD2)
Iron
Catecholamine, CK
K
Analytes decreased in afternoon
[CAPAI]
Cortisol
ACTH
Plasma renin
Aldosterone
Insulin
Analytes increased in afternoon
[PTAG]
PTH
TSH
ACP
GH
Analytes that require chilling
[LABI]
Lactic acid
Ammonia
Blood gas
iCa
Photosensitive analytes
[BC VNEC]
Bilirubin
CK
Vitamin A
Niacin
Erythrocyte protoporphyrin
Carotene
Specimen for electrolyte analysis
Heparinized Plasma
HbA1c specimen
EDTA Whole blood
AC of choice for lipoprotein
EDTA
AC of choice for blood gas studies
Lithium Heparin
Rate of glycolysis at room temp
7 mg/dL/hr
Rate of glycolysis at 4degC
2 mg/dL/hr
Whole blood glucose is _______% (higher/lower) than serum
10-15% lower
10% contamination with 5% dextrose increases glucose by _______ mg/dL
500
Reference method for:
Lipoprotein
Ultracentrifugation
Reference method for:
Cholesterol
Abell-Kendall
Latest: GC-MS
Reference method for:
TAG
Modified Van Handel and Zilversmit
Latest: GC-MS
Reference method for:
Glucose
Hexokinase
Reference method for:
GFR
Inulin Clearance
Reference method for:
BUN
Isotope Dilution Mass Spectrometry
Reference method for:
Creatinine
Isotope Dilution Mass Spectrometry
Reference method for:
Uric Acid
Isotope Dilution Mass Spectrometry
One step method for Chole
Liebermann-Burchardt
Three step method for Chole
Abell-Kendall
Arrange in ascending order (Glucose Levels)
Whole Blood
Venous
Capillary
Arterial
Whole Blood
Venous
Capillary = Arterial
