Microbe Control Flashcards
Understand how microbe growth is controlled by various agents and processes
What is the goal of sterilization?
The destruction of Bacterial endospores
Which organism is the most resistant?
Bacterial Endospores
Which organisms are the least resistant?
Enveloped Viruses
Which agents have the widest target range?
Heat and Radiation
Agents that are selective in their cellular targets tend to be…
more restricted in effectiveness
usually target a single cellular component
How many antimicrobial targets are there?
4
What are the targets for antimicrobials?
- Cell wall
- Cell Membrane
- Protein
- Nucelic Acids
How do antimicrobials interfere with cell walls?
Blocking synthesis
Digesting the cell wall
How do antimicrobials interfere with cell membranes?
bind to lipid layer, make a hole and disrupt cellular homeostasis
How do antimicrobials interfere with proteins
Causes denaturing
Prevents interaction between protein active site and substrate
How do antimicrobials interfere with nucleic acids
Acts as a mutagen
Keeps microbes from making proteins
What agents disrupt cell walls?
Chemicals
detergents
Alcohols
Surfactants
What agents disrupt cell membranes?
Detergents
What agents disrupts proteins?
Moist Heat
Alcohol
Phenolics
What agents disrupt nucelic acids?
Formalydhyde
Radiation
Ethelyne Oxide
Six (6) methods of physical control
Heat Cold Desiccation Radiation Filtration Osmotic Pressure
What are the types of heat?
Which heat is sterile?
Moist heat and Dry heat
Dry Heat
Microbe heat resisters are
Spores
Types of moist heat
Boiling
Pasturization
Non-pressure steam
Pressure steam
Is cold microstatic or microbial
Microstatic
Dessication is synonomous with
Dessication
Radiation types
Ionizing radiation
Non-ioninzing Radiation
HEPA filters, filter
air
Chemical control agents have _____ action in _______ concentration
Rapid; low
Chemical control agents must be ______ in water
soluble
Chemical control agents must have a ______ microbial range but be ______ to humans
broad; nontoxic
Should chemical agents be able to penetrate inanimate objects?
yes
Chemical agents should sanitize, deodorize, and be readily available and affordable?
Yes
What factors determine germicidal activity
Chemical concentration
Length of exposure
What are the levels of chemical decontamination
High- level germicide
Intermediate
Low
What are characteristics of low level germicides
kills vegatative microbes
some viruses
What are characteristics of intermediate level germicides
Kills FUNGAL spores
kills resistant pathogens
kills viruses
What is characteristic of high level germicides
killing endospores
If a germicide can kill endospores it is assumed that it can kill most other microbes
yes
What are the groups of germicidal sterilizers
Chlorine Iodine H2O2 Aldehydes gaseous sterilants
What are groups of germicidal disinfectants
Phenol Chlorohexidine Alcohol Detergents Heavy Metal compounds