Micro lecture 9

Question 1

state facts about quinolones as Nucleic acid synthesis inhibitors

Answer 1

Broad-spectrum, synthetic drugs containing the dual 4-quinolone rings

Act by inhibiting topoisomerases
DNA gyrase
Topoisomerase IV

(nalidixic acid - originator), ciprofloxacin, moxifloxacin

Question 2

how is resistance to quinolones are developed

Answer 2

Resistance can stem from:
Mutations in topoisomerase-encoding genes

Porin-poor outer membrane

Efflux pumps

Question 3

state facts about metronidazole as Nucleic acid synthesis inhibitors

Answer 3

Nitro group on core ring must be reduced for activity

Only achieved by anaerobes:
donate electrons to nitro group
(low redox potential inside cell)

Free radicals generated:
Damage to DNA & Cell death

Question 4

what is the structure for quinolones: memorize if you want.

Answer 4

look in slide 3

Question 5

what is the structure for metronidazole: memorize if you want.

Answer 5

look in slide 6