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Microbiology > Micro Lab Midterm Agars > Flashcards

Micro Lab Midterm Agars Flashcards

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1
Q

What is the acronym for Mannitol Salt Agar?

A

MSA

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2
Q

What is the acronym for Mueller Hinton Agar?

A

MH

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3
Q

What is the acronym for Blood Heart Infusion?

A

BHI

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4
Q

What is the acronym for Tryptic Soy Agar?

A

TSA

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5
Q

What is the acronym for Nutrient Agar?

A

NA

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6
Q

What is the acronym for MacConkey Agar?

A

MAC

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7
Q

Which agars are specialized?

A

Mannitol Salt Agar (MSA) & Mueller Hinton Agar (MH)

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8
Q

Which agars are selective AND differential?

A

MacConkey Agar (MAC) selects for gram negative and is differential based on the fermentation of lactose.

&

Mannitol Salt Agar (MSA) selects for gram positive and salt tolerance. Differentiates based on the fermentation of mannitol (sugar)

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9
Q

Which agar/s are yellow/ish?

A

Tryptic Soy Agar (TSA) & Nutrient Agar (NA)

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10
Q

Which agar is only used for antibiotic sensitivity testing?

A

Mueller Hinton (MH)

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11
Q

What is the name of the antibiotic sensitivity test/method?

A

Kirby Bauer or Disk Diffusion

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12
Q

What is the “Zone of Inhibition”?

A

It determines which antibiotic is resistant, susceptible or intermediate.

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13
Q

Which agar/s are all purpose?

A

Tryptic Soy Agar (TSA) & Nutrient Agar (NA)

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14
Q

Which agar turns colorless or pink depending on fermentation?

A

MacConkey Agar (MAC). It will turn pink if lactose is fermented and it will be colorless if lactose is not fermented.

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15
Q

Which agar is translucent?

A

Mueller Hinton (MH)

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16
Q

A general purpose agar?

A

Blood Heart Infusion (BHI)

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17
Q

Which medium is multi-purpose?

A

Thioglycolate Broth

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18
Q

Which agar is used to culture a variety of fastidious bacteria?

A

Blood Heart Infusion (BHI)

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19
Q

Which agar/s don’t grow fastidious bacteria?

A

Tryptic Soy Agar (TSA) & Nutrient Agar (NA)

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20
Q

Which differential medium determines the oxygen requirements for organisms?

A

Thioglycolate Broth

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21
Q

Which agar is a pale red/pink color?

A

MacConkey Agar (MAC)

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22
Q

Which agar is for bacteria that need amino acids/enzymes?

A

Blood Heart Infusion (BHI)

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23
Q

Does Mueller Hinton (MH) grow a variety of organisms?

A

Yes

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24
Q

Which agar will go from red to yellow based on fermentation?

A

Mannitol Salt Agar (MSA)

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25
Q

Which agar/s provide enough nutrients to allow a variety of organisms to grow?

A

Tryptic Soy Agar (TSA) & Nutrient Agar (NA)

26
Q

Which agar is found in brain and heart tissue?

A

Blood Heart Infusion (BHI)

27
Q

Pseudomonas aeruginosa turns what color on the Mueller Hinton (MH) agar plate?

A

Green

28
Q

Which agars are red in color?

A

Mannitol Salt Agar (MSA) & Blood Heart Inhibition (BH)

29
Q

What media has an oxygen concentration gradient built in?

A

Thioglycolate Broth

30
Q

Which agar can’t be used for antibiotic sensitivity testing and why?

A

Tryptic Soy Agar (TSA) & Nutrient Agar (NA) because the ions in the agar might inactivate the antibiotics

31
Q

Is Blood Heart Infusion selective or non-selective?

A

non-selective

32
Q

Which media grows anaerobes?

A

Thioglycolate Broth

33
Q

Which selects for gram positive and salt tolerance?

A

Mannitol Salt Agar (MSA)

34
Q

Mueller Hinton (MH) and Mannitol Salt Agar (MSA) is specialized?

A

Yes

35
Q

Tryptic Soy Agar (TSA) and Nutrient Agar (NA) are multi-pupose?

A

No, they are ALL PURPOSE

36
Q

MacConkey Agar (MAC) is translucent?

A

No, it is PALE RED/PINK in color

37
Q

Blood Heart Inhibition is provides enough nutrients to allow a variety of organisms to grow?

A

No, Tryptic Soy Agar (TSA) and Nutrient Agar (NA) provides enough nutrients to allow a variety of organisms to grow

38
Q

Mueller Hinton (MH) is only used for antibiotic sensitivity testing (KIrby Bauer; Zone of Inhibition)?

A

Yes

39
Q

Thioglycolate Broth creates an anaerobic environment?

A

Yes

40
Q

MacConkey Agar (MAC) selects for gram positive?

A

No, it selects for gram NEGATIVE

41
Q

Blood Heart Infusion is used for bacteria that need amino acids and enzymes as well as to culture fastidious organisms?

A

Yes

42
Q

Tryptic Soy Agar (TSA) and Nutrient Agar (NA) is a yellow or clear color?

A

Yes

43
Q

Mueller Hinton (MH) differentiates based on fermentation of lactose?

A

No, MacConkey Agar (MAC) differential based on fermentation of lactose

44
Q

Mannitol Salt Agar (MSA) is red

A

Yes

45
Q

Does Thioglycolate Broth differential medium determine the oxygen requirements for organisms?

A

Yes

46
Q

Blood Heart Infusion (BHI) differential based on the fermentation of mannitol?

A

No, Mannitol Salt Agar (MSA) differential based on the fermentation of mannitol

47
Q

MacConkey Agar (MAC) is translucent?

A

No, Mueller Hinton (MH) is translucent

48
Q

Nutrient Agar (NA) and Tryptic Soy Agar (TSA) is non-selective?

A

No, Blood Heart Infusion (BHI) is non-selective

49
Q

Mueller Hinton (MH) is red?

A

No, Blood Heart Infusion (BHI) is red

50
Q

Mannitol Salt Agar (MSA) doesn’t grow fastidious organisms?

A

No, Tryptic Soy Agar (TSA) and Nutrient Agar (NA) doesn’t grow fastidious agar

51
Q

Mannitol Salt Agar (MSA): If it ferments mannitol to acid, it will turn a yellow color?

A

Yes

52
Q

Blood Heart Inhibition (BHI) is found in the brain and heart tissues?

A

Yes

53
Q

MacConkey Agar (MAC): if ferments lactose, it will create an acidic environment and it will turn pink.

A

Yes

54
Q

Thioglycolate Broth grows a variety of organisms?

A

No, Mueller Hinton (MH) grows a variety of organisms

55
Q

Blood Heart Inhibition (BHI) is a general purpose?

A

Yes

56
Q

On a Mueller Hinton (MH) plate, Psuedomonas aeruginosa will turn green?

A

Yes

57
Q

Tryptic Soy Agar (TSA) and Nutrient Agar (NA) can’t be used for antibiotic sensitivity testing because the ions can inactivate the antibiotic?

A

Yes

58
Q

Thioglycolate Broth has an oxygen concentration gradient built in?

A

Yes

59
Q

Tryptic Soy Agar (TSA) and Nutrient Agar (NA) is multi-purpose?

A

No, Thioglycolate Broth is multi-purpose. TSA and NA are ALL PURPOSE

60
Q

How many medias and how many broths are we reviewing?

A

5 media and 1 broth

Microbiology (10 decks)

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