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Micro Lab Midterm Agars Flashcards

1
Q

What is the acronym for Mannitol Salt Agar?

A

MSA

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2
Q

What is the acronym for Mueller Hinton Agar?

A

MH

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3
Q

What is the acronym for Blood Heart Infusion?

A

BHI

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4
Q

What is the acronym for Tryptic Soy Agar?

A

TSA

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5
Q

What is the acronym for Nutrient Agar?

A

NA

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6
Q

What is the acronym for MacConkey Agar?

A

MAC

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7
Q

Which agars are specialized?

A

Mannitol Salt Agar (MSA) & Mueller Hinton Agar (MH)

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8
Q

Which agars are selective AND differential?

A

MacConkey Agar (MAC) selects for gram negative and is differential based on the fermentation of lactose.

&

Mannitol Salt Agar (MSA) selects for gram positive and salt tolerance. Differentiates based on the fermentation of mannitol (sugar)

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9
Q

Which agar/s are yellow/ish?

A

Tryptic Soy Agar (TSA) & Nutrient Agar (NA)

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10
Q

Which agar is only used for antibiotic sensitivity testing?

A

Mueller Hinton (MH)

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11
Q

What is the name of the antibiotic sensitivity test/method?

A

Kirby Bauer or Disk Diffusion

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12
Q

What is the “Zone of Inhibition”?

A

It determines which antibiotic is resistant, susceptible or intermediate.

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13
Q

Which agar/s are all purpose?

A

Tryptic Soy Agar (TSA) & Nutrient Agar (NA)

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14
Q

Which agar turns colorless or pink depending on fermentation?

A

MacConkey Agar (MAC). It will turn pink if lactose is fermented and it will be colorless if lactose is not fermented.

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15
Q

Which agar is translucent?

A

Mueller Hinton (MH)

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16
Q

A general purpose agar?

A

Blood Heart Infusion (BHI)

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17
Q

Which medium is multi-purpose?

A

Thioglycolate Broth

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18
Q

Which agar is used to culture a variety of fastidious bacteria?

A

Blood Heart Infusion (BHI)

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19
Q

Which agar/s don’t grow fastidious bacteria?

A

Tryptic Soy Agar (TSA) & Nutrient Agar (NA)

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20
Q

Which differential medium determines the oxygen requirements for organisms?

A

Thioglycolate Broth

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21
Q

Which agar is a pale red/pink color?

A

MacConkey Agar (MAC)

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22
Q

Which agar is for bacteria that need amino acids/enzymes?

A

Blood Heart Infusion (BHI)

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23
Q

Does Mueller Hinton (MH) grow a variety of organisms?

A

Yes

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24
Q

Which agar will go from red to yellow based on fermentation?

A

Mannitol Salt Agar (MSA)

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25
Which agar/s provide enough nutrients to allow a variety of organisms to grow?
Tryptic Soy Agar (TSA) & Nutrient Agar (NA)
26
Which agar is found in brain and heart tissue?
Blood Heart Infusion (BHI)
27
Pseudomonas aeruginosa turns what color on the Mueller Hinton (MH) agar plate?
Green
28
Which agars are red in color?
Mannitol Salt Agar (MSA) & Blood Heart Inhibition (BH)
29
What media has an oxygen concentration gradient built in?
Thioglycolate Broth
30
Which agar can't be used for antibiotic sensitivity testing and why?
Tryptic Soy Agar (TSA) & Nutrient Agar (NA) because the ions in the agar might inactivate the antibiotics
31
Is Blood Heart Infusion selective or non-selective?
non-selective
32
Which media grows anaerobes?
Thioglycolate Broth
33
Which selects for gram positive and salt tolerance?
Mannitol Salt Agar (MSA)
34
Mueller Hinton (MH) and Mannitol Salt Agar (MSA) is specialized?
Yes
35
Tryptic Soy Agar (TSA) and Nutrient Agar (NA) are multi-pupose?
No, they are ALL PURPOSE
36
MacConkey Agar (MAC) is translucent?
No, it is PALE RED/PINK in color
37
Blood Heart Inhibition is provides enough nutrients to allow a variety of organisms to grow?
No, Tryptic Soy Agar (TSA) and Nutrient Agar (NA) provides enough nutrients to allow a variety of organisms to grow
38
Mueller Hinton (MH) is only used for antibiotic sensitivity testing (KIrby Bauer; Zone of Inhibition)?
Yes
39
Thioglycolate Broth creates an anaerobic environment?
Yes
40
MacConkey Agar (MAC) selects for gram positive?
No, it selects for gram NEGATIVE
41
Blood Heart Infusion is used for bacteria that need amino acids and enzymes as well as to culture fastidious organisms?
Yes
42
Tryptic Soy Agar (TSA) and Nutrient Agar (NA) is a yellow or clear color?
Yes
43
Mueller Hinton (MH) differentiates based on fermentation of lactose?
No, MacConkey Agar (MAC) differential based on fermentation of lactose
44
Mannitol Salt Agar (MSA) is red
Yes
45
Does Thioglycolate Broth differential medium determine the oxygen requirements for organisms?
Yes
46
Blood Heart Infusion (BHI) differential based on the fermentation of mannitol?
No, Mannitol Salt Agar (MSA) differential based on the fermentation of mannitol
47
MacConkey Agar (MAC) is translucent?
No, Mueller Hinton (MH) is translucent
48
Nutrient Agar (NA) and Tryptic Soy Agar (TSA) is non-selective?
No, Blood Heart Infusion (BHI) is non-selective
49
Mueller Hinton (MH) is red?
No, Blood Heart Infusion (BHI) is red
50
Mannitol Salt Agar (MSA) doesn't grow fastidious organisms?
No, Tryptic Soy Agar (TSA) and Nutrient Agar (NA) doesn't grow fastidious agar
51
Mannitol Salt Agar (MSA): If it ferments mannitol to acid, it will turn a yellow color?
Yes
52
Blood Heart Inhibition (BHI) is found in the brain and heart tissues?
Yes
53
MacConkey Agar (MAC): if ferments lactose, it will create an acidic environment and it will turn pink.
Yes
54
Thioglycolate Broth grows a variety of organisms?
No, Mueller Hinton (MH) grows a variety of organisms
55
Blood Heart Inhibition (BHI) is a general purpose?
Yes
56
On a Mueller Hinton (MH) plate, Psuedomonas aeruginosa will turn green?
Yes
57
Tryptic Soy Agar (TSA) and Nutrient Agar (NA) can't be used for antibiotic sensitivity testing because the ions can inactivate the antibiotic?
Yes
58
Thioglycolate Broth has an oxygen concentration gradient built in?
Yes
59
Tryptic Soy Agar (TSA) and Nutrient Agar (NA) is multi-purpose?
No, Thioglycolate Broth is multi-purpose. TSA and NA are ALL PURPOSE
60
How many medias and how many broths are we reviewing?
5 media and 1 broth

Microbiology (10 decks)

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