Micro Agars/Broths

Question 1

Which type of media will grow most nonfastidious organisms? Examples include trypticase soy broth and nutrient agar (lecture 1)

Answer 1

Nutrient media

Question 2

Which type of media is supplemented with various substances (blood, blood by-products, vitamins, yeast extract, carbohydrates, etc.) and will grow fastidious organisms which have special nutritional requirements? Examples include Sheep blood agar plate and Chocolate agar. (lecture 1)

Answer 2

Enriched media

Question 3

Which media type enhances the growth of certain microorganisms while inhibiting other organisms? Examples include Gram-Negative broth and Selenite broth (lecture 1)

Answer 3

Enrichment broths

Question 4

This type of media contains antimicrobial substances such as dyes, antibiotics, and chemicals. It will inhibit some microbes while allowing the growth of others. Examples include PEA agar and CNA agar. (lecture 1)

Answer 4

Selective media

Question 5

Media containing no antimicrobial substances and allows growth of many organisms. Examples: BAP and CHOC

Answer 5

Nonselective media

Question 6

Media that aids in the identification of microorganisms by showcasing metabolic differences. Examples: carbohydrate fermentation media and enteric media

Answer 6

Differential media

Question 7

Agar: Polysaccharide melts at what temperature?

Answer 7

100C

Question 8

Agar: polysaccharide will stay liquid until what temperatures?

Answer 8

45-50C

Question 9

What media:
-selective & differential
-high salt concentration (7.5%)
-if the carbohydrate is fermented, acid is produced, causing agar to turn yellow from pH indicator phenol red
-assists in identification of S. aureus

Answer 9

Mannitol Salt Agar (MSA)

Question 10

Phenylethyl alcohol agar (PEA) and Colistin/Nalidixic acid agar (CNA) are used for GN or GP organisms?

Answer 10

Gram-positive