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MLT-MLS Fall 2022 > Lysine Iron Agar (LIA) > Flashcards

Lysine Iron Agar (LIA) Flashcards

1
Q

What is the purpose of the LIA test?

A

Determines if a microorganism can decarboxylate or deaminate the amino acid Lysine

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2
Q

TRUE or FALSE:
Deamination means removing the hydrogen group from an amino acid structure.

A

FALSE: deamination removes the amino group (NH3) from the amino acid

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3
Q

Deamination is an (aerobic/anaerobic) process which produces (amines/ammonia).

A

AEROBIC/AMMONIA

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4
Q

Deamination occurs in the (slant/butt) of the TSI agar

A

SLANT

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5
Q

TRUE or FALSE:
Decarboxylation mean removing the carboxyl group from an amino acids structure.

A

TRUE

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6
Q

Decarboxylation is an (aerobic/anaerobic) process which produces (amines/ammonia).

A

ANAEROBIC/AMINES

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7
Q

Decarboxylation occurs in the (slant/butt) of the TSI agar

A

BUTT

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8
Q

What carbohydrate is utilized in the LIA test to facilitate microorganism growth?

A

Glucose

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9
Q

What amino acid is utilized in the LIA test?

A

Lysine

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10
Q

What is the pH indication used in LIA?

A

Bromcresol purple

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11
Q

What color will LIA turn if only glucose is utilized?

A

Yellow (acid)

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12
Q

What color will LIA turn if carboxylation occurs?

A

Purple (Alkaline)

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13
Q

What color with LIA turn if deamination occurs?

A

Red (ammonia production)

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14
Q

What is the abbreviated reaction for this LIA result?

A

K/K

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15
Q

What is the abbreviated reaction for this LIA result?

A

K/K, H2S +

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16
Q

What is the abbreviated reaction for this LIA result?

A

R/A

17
Q

What is the abbreviated reaction for this LIA result?

A

K/A

18
Q

Which LIA result is Decarboxylase positive?
-K/K
-K/A
-R/A

A

K/K

19
Q

Which LIA result is decarboxylase and H2S positive?
-purple/purple
-red/purple
-purple/black
-purple/yellow

A

Purple/Black

20
Q

What colors will you expect see with a deaminase positive LIA?

A

Red/Yellow

21
Q

What colors would you expect to see with a decarboxylase and deaminase negative LIA test?

A

Purple/Yellow

22
Q

Which enterics will have an LIA result of K/K?

A

Klebsiella
Escherichia coli
Serratia

23
Q

Klebsiella, E. coli, and Serratia (deaminate/decarboxylate) lysine in LIA producing (ammonia/amines)

A

DECARBOXYLATE/AMINES

24
Q

Which enterics will have an LIA result of K/K, H2S+?

A

Salmonella

25
Q

Salmonella (deaminates/decarboxylates) lysine in LIA producing (ammonia/amines)

A

DECARBOXYLATES/AMINES

26
Q

Which enterics will have an LIA result of R/A?

A

Proteus
Providencia
Morganella

27
Q

Proteus, Providencia, and Morganella (deaminate/Carboxylate) lysine in LIA producing (ammonia/amines).

A

DEAMINATE/AMMONIA

28
Q

Which enterics will have an LIA result of K/A?

A

Shigella
Enterobacter

MLT-MLS Fall 2022 (10 decks)

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